ISSN:
1432-072X
Keywords:
Key words Soluble NAD-reducing hydrogenase
;
Membrane-bound hydrogenase
;
Diaphorase
;
Rhodococcus opacus 1b
;
Alcaligenes eutrophus H16
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract Six new strains of Alcaligenes enriched for and isolated as nickel-resistant bacteria resemble Alcaligenes eutrophus H16 and contain both an NAD-reducing, tetrameric soluble hydrogenase and a membrane-bound hydrogenase. None of the soluble hydrogenases share with the Rhodococcus opacus MR11 enzyme tetramer the property of being cleaved easily into two dimeric moieties [a hydrogenase (βδ) and an NADH:acceptor oxidoreductase (αγ)], in the absence of nickel or at low ionic strength. The soluble hydrogenase of the newly isolated strain MR22 of R. opacus equalled that of strain MR11. The absence of a membrane-bound hydrogenase in Alcaligenes denitrificans strain 4a-2 and in Alcaligenes ruhlandii was confirmed.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002030050431
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