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  • Allergen  (1)
  • Anaphylatoxins  (1)
  • CHD3  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Demonstration of neutrophil chemotactic anaphylatoxins in human dandruff (1989)
    Springer
    Archives of dermatological research 281 (1989), S. 482-486 
    Details
    ISSN: 1432-069X
    Keywords: Dandruff ; Anaphylatoxins ; C5a ; Chemotaxis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary In contrast to scales collected from the scalps of nine healthy individuals where a few parakeratotic cells are observable, a large number of parakeratotic cells associated with some infiltrated polymorphonuclear leukocytes (PMNLs) were found in the scales obtained from 11 individuals complaining of dandruff. Therefore, we determined the neutrophil chemotactic properties of the water-soluble extracts of dandruff scales and normal control scalp scales. Aqeous extracts fractionated by Sephadex G-75 showed a potent chemotactic activity only in the fractions of the dandruff patients that eluted with cytochrome C marker (cyt C; molecular weight, 12 kDa). It was comparatively stable to heat but was greatly inhibited by the addition of anti-C5 antiserum. Radioimmunoassay demonstrated that, although small amounts of C5a and C4a anaphylatoxins were demonstratable even in the extracts of normal scalp, they were found in significantly increased amounts in the extracts of dandruff. Moreover, there was a significantly positive correlation between C5a and C4a concentrations in these extracts. These results suggest that classical complement pathway activation with resultant production of C5a anaphylatoxin is involved in the migration of PMNLs into the lesional skin of dandruff.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00510084
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Lack of a role for bradykinin in allergen-induced airway microvascular leakage and bronchoconstriction in the guinea pig (1996)
    Springer
    Inflammation research 45 (1996), S. 123-126 
    Details
    ISSN: 1420-908X
    Keywords: Airway ; Allergen ; Bradykinin ; Bradykinin receptor ; HOE140
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract We have investigated the role of bradykinin in allergen-induced airway microvascular leakage and bronchoconstriction in sensitized guinea pigs. We used a selective bradykinin B2 receptor antagonist, HOE140, which has been shown to prevent the airway effects induced by bradykinin. Lung resistance (RL) was measured for 6 min after challenge with allergen. Extravasation of Evans blue dye into airway tissues was used as an index of the airway microvascular leakage. Aerosolized ovalbumin (5 mg/ml, 30 breaths) induced a significant increase in RL and leakage of dye in the trachea, main bronchi and intrapulmonary airways in the ovalbumin-sensitized guinea pigs. HOE140 given by inhalation (200 μM, 60 breaths) had no effect on the airway microvascular leakage and bronchoconstriction induced by the allergen. I.v. HOE140 (200 nmol/kg) did not significantly inhibit these airway responses. We conclude that bradykinin-mediated mechanism may not play a significant role in airway microvascular leakage or bronchoconstriction induced by allergen.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02265164
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Induction of mRNAs in response to acclimation of trout cells to different osmolalities (2000)
    Springer
    Fish physiology and biochemistry 22 (2000), S. 255-262 
    Details
    ISSN: 1573-5168
    Keywords: CHD3 ; cell culture ; complement ; differential mRNA display polymerase chain reaction ; growth ; nucleolin ; protein kinase ; rainbow trout ; RTG-2 cell ; salinity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The direct effect of osmolality on growth and mRNA population were investigated in the rainbow trout cell line (RTG-2). These cells can grow in the media of osmolalities ranging from 200 to 600 mosmol kg-1. With two-dimensional electrophoresis, the in vitro translation of poly(A+) RNA isolated from these cells showed osmoresponsive changes in the population of translatable mRNAs. Using differential mRNA display polymerase chain reaction, however, we identified inducible cDNA products in hyper-osmotic and hypo-osmotic media as third component of complement, and as homologues of known genes: an atypical protein kinase regulated by the thyrotropin-dependent mitogenic pathway, nucleolin and CHD3. The remaining cDNAs have no significant homology in GenBank. Northern blots demonstrate that their mRNA levels were induced in hyper-osmotic and hypo-osmotic media, but not by other stresses. The expressed proteins of these mRNAs may be involved directly or indirectly in the adaptation of RTG-2 cells to different osmolalities probably through the osmotic signal transduction and adjustment in cellular metabolism to osmotic stress.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1007843508043
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    Paper (German National Licenses)
    Fulltext
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