ISSN:
1432-0983
Keywords:
Key words APS kinase
;
ATP sulphurylase
;
Aspergillus nidulans
;
Allosteric inhibition
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract The sD gene of Aspergillus nidulans has been cloned by heterologous screening of rationally selected cosmids. Co-transformation of the sD50 mutant JMP1 confirmed the presence of a functional gene. Sequence analysis determined this gene to be 680 bp in length, containing a 59-bp intron and encoding a protein of 206 amino acids. A protein-sequence comparison revealed a similarity to the C-terminal region of ATP sulphurylase, the sC gene product. Further sequence comparison revealed differences in a consensus sequence ATP-binding motif, indicating non-functionality of the APS kinase-like domain of ATP sulphurylase, and confirms sD as the gene encoding APS kinase in A. nidulans.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/s002940050295
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