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  • 1
    ISSN: 1432-0428
    Keywords: Key words High glucose ; adhesion molecules ; endothelial cells ; interleukin-1 ; diabetes mellitus.
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We evaluated the influence of high ambient glucose on cellular expression of adhesion molecules, known to mediate endothelial interaction of leucocytes and monocytes. Paired cultures of individual isolates of human umbilical vein endothelial cells (HUVECs) were studied by fluorescence activated cell sorter analysis after exposure to 30 vs 5 mmol/l glucose. Incubation of HUVECs for 24 h in 30 mmol/l glucose increased ICAM-1 (intercellular adhesion molecule-1; 116.4 ± 16.9 % of control, p≤ 0.05), but not PECAM (platelet endothelial cell adhesion molecule) expression, compared to cultures kept in 5 mmol/l glucose. Long-term exposure (13 ± 1 days) of HUVECs to 30 mmol/l glucose increased expression of ICAM-1 to 122.5 ± 32.2 % (p 〈 0.002) and reduced that of PECAM to 86.9 ± 21.3 % vs the respective control culture in 5 mmol/l glucose (p 〈 0.02). Stimulation of confluent HUVECs, kept in 30 vs 5 mmol/l glucose for 13 ± 1 days, with 20 U/ml interleukin-1 for 24 h (ICAM-1) and 4 h (endothelial leukocyte adhesion molecule 1) resulted in reduced ICAM-1 (84.8 ± 27.0 %, p 〈 0.05) and endothelial leukocyte adhesion molecule-1 (87.6 ± 22.4 %, p 〈 0.05) expression vs control cells, while that of PECAM (t: 24 h) and vascular cell adhesion molecule-1 (t: 16 h) remained unchanged. In conclusion, it appears that differences in expression of adhesion molecules on HUVECs in response to high glucose reflects endothelial glucose toxicity, which may also induce endothelial dysfunction in diabetes. [Diabetologia (1995) 38: 1367–1370]
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-0428
    Keywords: High glucose ; adhesion molecules ; endothelial cells ; interleukin-1 ; diabetes mellitus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary We evaluated the influence of high ambient glucose on cellular expression of adhesion molecules, known to mediate endothelial interaction of leucocytes and monocytes. Paired cultures of individual isolates of human umbilical vein endothelial cells (HUVECs) were studied by fluorescence activated cell sorter analysis after exposure to 30 vs 5 mmol/l glucose. Incubation of HUVECs for 24 h in 30 mmol/l glucose increased ICAM-1 (intercellular adhesion molecule-1; 116.4±16.9% of control, p ≤ 0.05), but not PECAM (platelet endothelial cell adhesion molecule) expression, compared to cultures kept in 5 mmol/l glucose. Long-term exposure (13±1 days) of HUVECs to 30 mmol/l glucose increased expression of ICAM-1 to 122.5±32.2% (p〈0.002) and reduced that of PECAM to 86.9±21.3% vs the respective control culture in 5 mmol/l glucose (p〈0.02). Stimulation of confluent HUVECs, kept in 30 vs 5 mmol/l glucose for 13±1 days, with 20 U/ ml interleukin-1 for 24 h (ICAM-1) and 4 h (endothelial leukocyte adhesion molecule 1) resulted in reduced ICAM-1 (84.8±27.0%, p〈0.05) and endothelial leukocyte adhesion molecule-1 (87.6±22.4%, p〈0.05) expression vs control cells, while that of PECAM (t: 24 h) and vascular cell adhesion molecule-1 (t: 16 h) remained unchanged. In conclusion, it appears that differences in expression of adhesion molecules on HUVECs in response to high glucose reflects endothelial glucose toxicity, which may also induce endothelial dysfunction in diabetes.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-0428
    Keywords: Alloxan-diabetes in rat ; weight gain ; weight loss ; blood sugar ; immunoreactive insulin in serum ; weight of the adrenals
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé 7–9 semaines après l'administration d'alloxane il y avait deux groupes de rats diabétiques; un qui avait gagné en moyenne 60 g et l'autre qui avait perdu en moyenne 28 g. — Malgré cette différence de poids corporel, le taux d'insuline immunoréactive dans le sérum (IRI) et le taux du sucre dans le sang ne différaient pas significativement dans les deux groupes. — Ces résultats montrent, qu'une augmentation de poids corporel est possible chez les rats rendus chroniquement diabétiques par l'alloxane malgré une forte hyperglycémie et une diminution marquée d'IRI. — Chez les rats chroniquement diabétiques qui avaient perdu du poids corporel, le poids relatif des surrénales était plus élevé que chez les autres diabétiques ou chez les rats normaux.
    Abstract: Zusammenfassung Alloxandiabetische Ratten wurden 7–10 Wochen nach der Alloxangabe in eine Gruppe mit einer mittleren Gewichtszunahme von 60 g und in eine zweite mit einer Gewichtsabnahme von durchschnittlich 28 g geteilt. — Trotz der stark unterschiedlichen Gewichtsver änderung war kein statistisch erfaßbarer Unterschied bezüglich der Konzentration des IRI und des Blutzuckers zwischen den beiden Gruppen festzustellen. — Die Ergebnisse zeigen, daß auch bei chronisch alloxandiabetischen Ratten mit ausgeprägter Hyperglykämie und stark erniedrigten Serum-IRI eine Gewichtszunahme möglich ist. — Bei den abgemagerten diabetischen Ratten war das relative Gewicht der Nebennieren höher als bei den diabetischen Ratten mit Gewichtszunahme bzw. bei den Kontrollen.
    Notes: Summary 7–9 weeks after alloxan administration it was possible to divide the diabetic rats into two groups : one with a gain in body weight (mean gain 60 g) and the other with a loss of body weight (mean loss 28 g). — In spite of this striking difference in body weight, there was no significant difference in the concentration of immunoreactive insulin of the serum (IRI) nor in the blood sugar levels between the two groups. — The results show that gain in body weight is possible even in chronically alloxandiabetic rats with marked hyperglycaemia and with a distinct diminution of IRI. — In the chronically diabetic rats with weight loss the relative weight of the adrenals was increased in comparison with the values found in normal rats or in the other group of alloxan-diabetic rats.
    Type of Medium: Electronic Resource
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