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  • Analyse von Aminosäuren undα-Ketosäuren  (1)
  • Anterior cruciate insufficiency  (1)
  • Cerebrospinal fluid  (1)
  • 1
    ISSN: 1432-1459
    Keywords: Myelin basic protein ; Cerebrospinal fluid ; Multiple sclerosis ; Radioimmunoassay
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Mit Hilfe eines Radioimmunoassays wurde der Liquor von 105 Patienten auf Substanzen untersucht, die mit dem Fragment 89–169 des bovinen basischen Myelinproteins (BP) kreuzreagieren. Von 72 Patienten mit Multipler Sklerose hatten 52 einen höheren BP-Gehalt im Liquor als die Kontrollgruppe, d.h. mehr als 2 ng/ml. Dies war bei fast all jenen Patienten der Fall, bei denen der Liquor innerhalb von 2 Wochen nach einem akuten Schub bzw. zum Zeitpunkt einer deutlichen Verschlechterung des Zustandes bei der chronisch-progredienten Form gewonnen wurde. Der BP-Gehalt des Liquors nimmt zwischen dem 15. und 30. Tag nach Schubbeginn deutlich ab. In der schleichenden progredienten Form wurde mit einer Ausnahme kein erhöhter BP-Gehalt gefunden. Das basische Myelinprotein ist aber auch im Liquor von Patienten mit anderen neurologischen Erkrankungen zu finden. Hervorzuheben ist das Auftreten von BP im Liquor von Patienten mit Retrobulbär-neuritis. Der Nachweis von mit Bruchstück 89–169 kreuzreagierenden Substanzen im Liquor ist infolgedessen zwar nicht spezifisch für die Multiple Sklerose, ist aber ein wertvoller Parameter in der Diagnose und Einschätzung der MS.
    Notes: Summary Cerebrospinal fluid (CSF) from 105 patients was analyzed by radio-immunoassay for the presence of material cross-reactive with peptide 89–169 of bovine myelin basic protein (BP). In a group of 72 multiple sclerosis patients, 52 showed higher BP content than the control group, i.e. more than 2 ng/ml CSF. Increased BP or BP fragments could be detected in CSF from almost all patients who recently (within 2 weeks) had had an acute episode, or after deterioration in the progressive form of the disease. Fifteen to 30 days after the onset of exacerbation or in a stable period, BP content decreases and in the slowly progressive form was in the range of the control group with one exception. BP content was also elevated in the CSF of patients with other neurological diseases. The presence of BP in the CSF from patients with isolated retrobulbar neuritis is of particular interest. Thus the presence of material cross-reactive with BP fragment 89–169 is not specific for multiple sclerosis, but is a useful parameter in diagnosis and evaluation of MS.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Knee surgery, sports traumatology, arthroscopy 2 (1994), S. 85-87 
    ISSN: 1433-7347
    Keywords: Anterior knee pain ; Anterior cruciate insufficiency ; Hypermobility ; Limited hip range of movement
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine , Sports Science
    Notes: Abstract From 1983 to 1990, 82 knee arthroscopies (8.2%) carried out in our patients found nothing pathological. Sixty-four percent of these patients were active in sports, but trauma was noted in 32% of the cases only. Football and other ball games, skiing, and track and field athletics were the main causes. Twenty-six percent of the patients had undergone previous surgery in the affected knee. At a mean of 4.6 years postoperatively, clinical and radiological re-assessment was conducted so as to compare our pre- and intraoperative findings with the further course of events. We found that 48.2% of the patients were symptom-free after the diagnostic arthroscopy, 37.5% had persistent discomfort and 14.3% had a recurrence of discomfort after 6 months to 2 years. The objective measurement score (Zarins Rowe score), at 47.5 out of 50 points, was better than the subjective score, at 40 out of 50 points. Our diagnoses had to be changed retrospectively: meniscal lesions were diagnosed too frequently, while chondropathia patellae and instability were often missed. Additionally, complaints could be related to abnormal axis, limited range of motion of the hip or knee, leg length inequality and hypermobility. Being unable to verify a presumed intra-articular lesion arthroscopically is frustrating for both doctor and patient. Our data suggest that meniscal signs should be looked at more critically and emphasise the need for a complete evaluation of the whole locomotor system.
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1618-2650
    Keywords: Analyse von Aminosäuren undα-Ketosäuren ; Chromatographie ; Ionenaustausch ; Pyridoxylderivate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology
    Description / Table of Contents: Zusammenfassung Zur Analyse von Aminosäuren werden deren Pyridoxylderivate hergestellt und durch Flüssigkeits-Chromatographie getrennt. Die Bestimmung im Eluat erfolgt über die UV- oder Fluorescenzspektren oder durch Aktivitätsmessung, wenn radioaktives NaBT4 bei der Darstellung verwendet wird. Diese letzte Methode ist die empfindlichste. Die Empfindlichkeit kann bis zu 10−13 Mol gesteigert werden (Fehler ± 5%). Das Verfahren kann ebenfalls für die Analyse vonα-Ketosäuren eingesetzt werden.
    Notes: Abstract The synthesis of pyridoxyl-amino acids and their separation by liquid chromatography is described. The determination of the compounds in the eluate can be performed optically via the UV- or fluorescence spectra or radiochemically by activity measurement when radioactive NaBT4 is used in the preparation. The latter method is the most sensitive one. Sensitivity can be increased up 10−13 Mol (error ± 5%). The procedure described can also be applied to the separation and determination ofα-keto acids.
    Type of Medium: Electronic Resource
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