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  • Biochemistry and Biotechnology  (6)
  • Chemical Engineering  (3)
  • Analytical Chemistry and Spectroscopy  (1)
  • Electrophoresis  (1)
  • 1
    ISSN: 1432-2242
    Keywords: Tissue culture ; Electrophoresis ; Storage proteins ; Mutation ; Triticum aestivum
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Fertile r0 plants of the winter wheat line ND7532 (Triticum aestivum L.) were regenerated from callus tissue after 60–190 days in culture. Seeds produced from these self-pollinated plants were planted in the field. Of the 5586 R1 plants, 32 differed for one or more agronomic traits from plants not passed through tissue culture process. Gliadin electrophoregrams were prepared from bulk samples of R2 seed from these 32 plants. Four of the 32 produced gliadin patterns different from controls, so 12 seeds of each of these four lines were examined individually. Three of the four mutant lines were fixed for the presence of a mutant protein of 50 relative mobility units (RMU) and the corresponding loss of a parental protein of 26 RMU. The remaining line segregated for the presence/absence of band 50 and the corresponding loss/retention of band 26. The mutant protein of 50 RMU was never seen in control plants. This indicated that either band 50 was coded for by a mutant gene allelic to the gene that coded for band 26 or that bands 26 and 50 were coded for by two different structural alleles under the control of a common regulatory locus. Each of the 12 seeds from the four mutant lines contained a prominent protein band at 30 (RMU), which was only observed as a faint band in one control seed. The types of variation in gliadin patterns observed in somaclones of ND7532 were similar to those reported for the line ‘Yaqui 50E’, except that, gliadin changes occurred less frequently in ND7532.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 30 (1987), S. 882-886 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The presence of anions in solution was found to inhibit the uptake of La3+, Cd2+, Pb2+, UO2+2, and Ag+ by Rhizopus arrhizus biomass. The effects ranged from total inhibition of Cd2+ and Pb2+ uptake at equimolar concentrations of EDTA to no change in uptake of La3+ or UO2+2 at 12-fold molar excesses of Cl- or CO2-3. No anion was found to enhance metal uptake levels, and the degree of inhibition generally followed the series: \documentclass{article}\pagestyle{empty}\begin{document}$${\rm EDTA } \ge \ge {\rm SO}_{^{^{^{\rm 4} } } }^{{\rm 2} - } \ge {\rm Cl}^ - \ge {\rm PO}_{^{^{^{\rm 4} } } }^{{\rm 3} - } \ge {\rm glutamate} \ge {\rm CO}_{^{^{\rm 3} } }^{{\rm 2} - } $$\end{document} The chemical equilibrium model REDEQL2 was adapted to treat metal uptake by R. arrhizus biomass and used to predict the effects of anions in solution. Comparisons with the experimental results are made and discussed in light of the assumptions underlying the model.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 24 (1982), S. 165-175 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A. paraffineus ATCC 19558 grown in MMSM (modified mineral salts medium) containing hydrocarbon produced surfactant, with a maximum CMC-1 value obtained by using hexadecane as the carbon source. No activity of surface active agent in whole broth was observed when glucose was used in the MMSM instead of hexadecane. The biomass concentration obtained with glucose was about 40% of that obtained with hexadecane. Glucose (4%) in the medium contaning hexadecane caused a 27 and 21% decrease of biomass and surfactant concentrations, respectively. In the process of surfactant production, glucose can be used as a carbon source for growth, and hexadecane added later can serve for production of the surface active agent. The optimum temperature for production of surfactant is 27°C.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 31 (1988), S. 282-286 
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 46 (1995), S. 180-183 
    ISSN: 0006-3592
    Keywords: self-cycling fermentation ; mathematical model ; synchronous growth ; computer-controlled fermentation ; fermentation ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: This article presents a mathematical model for biomass, limiting substrate, and dissolved oxygen concentrations during stable operation of self-cycling fermentation (SCF). Laboratory experiments using the bacterium Acinetobacter calcoaceticus RAG-1 and ethanol as the limiting substrate were performed to validate the model. A computer simulation developed from the model successfully matched experimental SCF intracycle trends and end-of-cycle results and, most importantly, settled into an unimposed periodicity characteristic of stable SCF operation. © 1995 John Wiley & Sons, Inc.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 40 (1992), S. 86-90 
    ISSN: 0006-3592
    Keywords: aqueous two-phase system ; biosurfactant ; extractive bioconversion ; cell partitioning ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The partition of surfactants and a biosurfactant-producing microorganism was studied in polyethylene glycol and dextran aqueous two-phase systems. In the presence of sodium phosphate, surfactants distributed themselves according to charge. Cationic surfactants preferred the bottom phase, while anionic surfactants were attracted to the top phase. Incresing the phosphate molarity or the pH resulted in a more 1-sided surfactant partitioning. Biosurfactant partitioning was weaker than synthetic surfactant partitioning due to the weaker effective charge and lack to strong specific affinity for any of the phase-forming polymers. Bacillus Subtilis cells partitioned very storngly to the bottom phase. The bioscurfactant, surfactin, produced by this microorganism partitioned to the top phase. Batch fermentations were carried out in an aqueous 2-phase system. Surfactin was produced in larger quanities in the 2-phase fermentation than in the regular mineral salts medium.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 31 (1985), S. 1202-1212 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Significant noise (random error) is common in an on-line signal when measuring the particle size distribution in a fluidized bed. The application of optimal estimation theory to obtain a filtering algorithm that can rectify such measurements is detailed. Off-line simulation studies demonstrate that the filtering algorithm yields a measure of particle size distribution that is well-behaved, accurate, and able to track in real time as conditions change in the bed.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 32 (1986), S. 186-194 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Optimal distributed-parameter filters are commonly implemented using approximating lumped Kalman filtering theory. The effect of such an approximation is investigated. A theoretical development shows that there is a loss in the spatial noise correlation for the lumped approximants. Two numerical examples of engineering significance illustrate that one result of this loss is slower filter convergence for the lumped approximants relative to the full distributed-parameter filters.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 32 (1986), S. 389-396 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A novel method for tracking particle size distribution dynamics in a fluidized bed yields on-line, real-time measurements of particle size distribution that are representative of actual conditions. These measurements are somewhat corrupted with noise (random error), however, and are available to the computer only at discrete time intervals. An optimal filtering algorithm combines the measurements with predictions from an idealized dynamic model to yield particle size distribution estimates that are not only on-line and real-time, but also virtually continuous and well-behaved.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 51 (1996), S. 112-119 
    ISSN: 0006-3592
    Keywords: biodegradation ; self-cycling fermentation ; phenol ; Pseudomonas putida ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Self-cycling fermentation (SCF) in a stirred tank reactor was applied to the biodegradation of phenol by Pseudomonas putida. The technique resulted in stable and repeatable performance. Complete substrate consumption was achieved under all operating conditions investigated. SCF resulted in substrate utilization rates as high as 14.5 kg of phenol per cubic meter of fermentor volume per day of fermentation, higher than those that have been reported for batch, CSTR, and packed column fermentors. A mathematical model of the self-cycling fermentation process was expanded to include inhibitory substrate-microorganism combinations, and was shown to provide a good fit to both end-of-cycle and intracycle experimental data. © 1996 John Wiley & Sons, Inc.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
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