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  • 1
    Electronic Resource
    Electronic Resource
    Restricted infection with canine distemper virus leads to down-regulation of myelin gene transcription in cultured oligodendrocytes (1995)
    Springer
    Acta neuropathologica 90 (1995), S. 312-318 
    Details
    ISSN: 1432-0533
    Keywords: Canine distemper virus ; Oligodendrocytes ; Myelin gene expression ; Demyelination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Canine distemper virus (CDV) induces oligodendroglial degeneration and multifocal demyelination in the central nervous system. The mechanism of oligodendrocyte degeneration is not understood but it has been shown that there is a restricted infection of these cells without viral protein production. Using a combination of immunocytochemistry and in situ hybridization we were able to demonstrate the transcription of the entire virus genome throughout the whole observation period (7–35 days after infection) in oligodendrocytes in CDV-infected brain cell cultures. Therefore, the lack of viral protein and particle production can not be explained on the basis of a defective viral transcription. The present study also shows that a restricted infection of oligodendrocytes with CDV down-regulates the transcription of the major myelin genes coding for proteolipid protein, myelin basic protein (MBP) and myelin-associated glycoprotein in a very similar way. Using densitometry for in situ hybridization products of MBP in populations of normal and infected oligodendrocytes, an effect could be observed long before morphological changes were detectable. The present results strongly suggest that demyelination in distemper is induced by a restricted CDV infection of oligodendrocytes which down-regulates the expression of a variety of cellular genes, in particular those coding for myelin proteins. Consequently, the infected cells are no longer able to synthesize all the membrane compounds which are necessary for maintaining their structural integrity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00296516
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Restricted infection with canine distemper virus leads to down-regulation of myelin gene transcription in cultured oligodendrocytes (1995)
    Springer
    Acta neuropathologica 90 (1995), S. 312-318 
    Details
    ISSN: 1432-0533
    Keywords: Key words Canine distemper virus ; Oligodendrocytes ; Myelin gene expression ; Demyelination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Canine distemper virus (CDV) induces oligodendroglial degeneration and multifocal demyelination in the central nervous system. The mechanism of oligodendrocyte degeneration is not understood but it has been shown that there is a restricted infection of these cells without viral protein production. Using a combination of immunocytochemistry and in situ hybridization we were able to demonstrate the transcription of the entire virus genome throughout the whole observation period (7–35 days after infection) in oligodendrocytes in CDV-infected brain cell cultures. Therefore, the lack of viral protein and particle production can not be explained on the basis of a defective viral transcription. The present study also shows that a restricted infection of oligodendrocytes with CDV down-regulates the transcription of the major myelin genes coding for proteolipid protein, myelin basic protein (MBP) and myelin-associated glycoprotein in a very similar way. Using densitometry for in situ hybridization products of MBP in populations of normal and infected oligodendrocytes, an effect could be observed long before morphological changes were detectable. The present results strongly suggest that demyelination in distemper is induced by a restricted CDV infection of oligodendrocytes which down-regulates the expression of a variety of cellular genes, in particular those coding for myelin proteins. Consequently, the infected cells are no longer able to synthesize all the membrane compounds which are necessary for maintaining their structural integrity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00296516
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Low-temperature study of dynamic effects in tetramethyllimonene (1990)
    Chichester : Wiley-Blackwell
    Organic Magnetic Resonance 28 (1990), S. 205-211 
    Details
    ISSN: 0749-1581
    Keywords: Tetramethyllimonene ; Assignments ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Dynamic effects of tetramethyllimonene were studied at low temperatures. Nuclear Overhauser effects measured under optimized conditions at 223 K and the results of 2D correlation experiments allowed the identification of two conformers and the assignment of all the proton and carbon resonances. For the determination of exchange and relaxation rates the method of saturation transfer was applied. At temperatures below 223 K further dynamic effects were recognized. Structural information obtained experimentally was compared with the results of Allinger force field calculations.
    Additional Material: 8 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrc.1260280303
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    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Sequential proton saturation for the heteronuclear 1D NOE experiment (1992)
    Chichester : Wiley-Blackwell
    Organic Magnetic Resonance 30 (1992), S. 466-475 
    Details
    ISSN: 0749-1581
    Keywords: Heteronuclear NOE ; Selective pulses ; Shaped pulses ; Sequential excitation ; Improved data accumulation ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: An improved 1D pulse sequence for detecting heteronuclear NOEs on a qualitative level is described and demonstrated. It makes use of shaped selective pulses and takes advantage of sequential perturbation of several proton resonances during one experiment and correspondingly more efficient data accumulation schemes. The method is particularly suitable for assigning quaternary carbons and for establishing connectivities between assigned molecular fragments bound to these quaternary centres.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrc.1260300603
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    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Conformational analysis of cembrenenol: Application of 1H and 13C NMR spectroscopy and of computational methods (1991)
    Chichester : Wiley-Blackwell
    Organic Magnetic Resonance 29 (1991), S. 834-847 
    Details
    ISSN: 0749-1581
    Keywords: Cembrenenol ; Cyclic diterpene ; Conformational analysis ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: The solution structure of the cyclic diterpene cembrenenol was investigated. A strategy for conformational analysis is proposed which is based on computational methods and experiments to determine the structure-relevant NMR parameters. Computed and experimentally determined steady-state NOEs were compared in a first step, which served to sort out a few probable conformers. The detailed analysis of homo- and hetero-nuclear coupling information together with NOE data defining local geometries allowed, in a second step, the selection of the most probable conformation. The structure corresponds to the lowest energy structure proposed by computational methods alone. Its ring structure is in excellent agreement with the x-ray ring structure of the closely related cembrenene.
    Additional Material: 6 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrc.1260290815
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    Paper (German National Licenses)
    Fulltext
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