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  • Analytical Chemistry and Spectroscopy  (1,947)
  • Engineering General  (787)
  • 1
    Electronic Resource
    Electronic Resource
    Is Multidimensional High Performance Liquid Chromatography (HPLC) an Alternative in Protein Analysis to 2D Gel Electrophoresis? (2000)
    Weinheim : Wiley-Blackwell
    Journal of High Resolution Chromatography 23 (2000), S. 259-265 
    Details
    ISSN: 0935-6304
    Keywords: Proteomics ; protein analysis ; multidimensional HPLC ; ion-exchange chromatography ; reversed phase chromatography ; comprehensive HPLC ; two-dimensional HPLC ; Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: ---The interactive modes of High Performance Liquid Chromatography (HPLC) of proteins provide a platform for the construction of a multidimensional HPLC system coupled to mass spectrometry. We present a system composed of both anion and cation exchanger columns, in the first dimension, and n-octadecyl bonded 1.5 μm nonporous silica columns in the second dimension. Both columns are operated under gradient conditions. A system suitability test with standard proteins showed that the total analysis can be performed within about 20 minutes. The fractions taken from the ion exchanger column are directly analyzed within one minute on the reversed phase column at a high flow rate. Two reversed phase columns are applied and operated alternatively: while the first column performs the separation within one minute, the analytes leaving the first dimension are enriched in an on-column focusing mode on top of the second column. The sample clean-up and enrichment is performed on a novel type of restricted access cation exchanger column with internal sulfonic acid groups and external diol groups. The columns exhibit a molecular weight exclusion limit for globular proteins of about 15 kDa. Our next studies will be directed towards the analysis of proteins and peptides from extracts of fibroblasts.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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    Paper (German National Licenses)
  • 2
    Electronic Resource
    Electronic Resource
    Carbon-13 NMR spectra of some labdane diterpenoids (1981)
    Chichester : Wiley-Blackwell
    Organic Magnetic Resonance 17 (1981), S. 301-302 
    Details
    ISSN: 0030-4921
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: Carbon-13 signal assignments of the labdane diterpenoids phlogantholide-A, its diacetate and isophlogantholide-A are reported. The assignments of the 13C NMR signals of 14-deoxyandrographolide and its diacetate, made earlier, have now been confirmed by lanthanide shift studies on the former and also by 13C spectral studies on anhydroandrographolide diacetate and 14-deoxy-11,12-didehydroandrographolide diacetate.
    Additional Material: 1 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mrc.1270170416
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  • 3
    Electronic Resource
    Electronic Resource
    A study on one-dimensional consolidation of layered systems (1992)
    New York, NY [u.a.] : Wiley-Blackwell
    International Journal for Numerical and Analytical Methods in Geomechanics 16 (1992), S. 815-831 
    Details
    ISSN: 0363-9061
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Architecture, Civil Engineering, Surveying , Geosciences
    Notes: A general analytical solution, which is more explicit than the one given by Schiffman and Stein,1 for the problem of one-dimensional consolidation of layered soils is presented. A relevant computer program is developed and the computed results on some examples are included. From these results, an in-depth study on the one-dimensional consolidation behaviour of layered systems is then made. It is demonstrated through these examples that both the solution technique and the computer program developed are very efficient. It is found that the effects of coefficients of permeability and volume compressibility of soil on the consolidation of layered systems are different and cannot be embodied into the coefficient of consolidation of soil. The stiffness of soil layer also plays an important role on the rate of consolidation.
    Additional Material: 15 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/nag.1610161104
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  • 4
    Electronic Resource
    Electronic Resource
    A fully coupled non-linear dynamic analysis procedure and its verification using centrifuge test results (1994)
    New York, NY [u.a.] : Wiley-Blackwell
    International Journal for Numerical and Analytical Methods in Geomechanics 18 (1994), S. 305-325 
    Details
    ISSN: 0363-9061
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Architecture, Civil Engineering, Surveying , Geosciences
    Notes: The equations governing the dynamic behavior of saturated porous media as well as a finite element spatial discretization of these equations are summarized. A three-parameter time integration scheme called the Hilber-Hughes-Taylor α-method is used together with a predictor/multi-corrector algorithm, instead of the widely used Newmark's method, to integrate the spatially discrete finite element equations. The new time integration scheme possess quadratic accuracy and desirable numerical damping characteristics. The proposed numerical solution and bounding surface plasticity theory to describe the constitutive behaviour of soil have been implemented as the computer code DYSAC2. Predictions made by DYSAC2 code are verified using dynamic centrifuge test results for a clay embankment. Importance of initial state of a soil on its dynamic behaviour is demonstrated.
    Additional Material: 12 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/nag.1610180503
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  • 5
    Electronic Resource
    Electronic Resource
    A gas chromatographic mass spectrometric assay for plasma trifluoperazine concentrations following single doses (1982)
    Chichester : Wiley-Blackwell
    Biological Mass Spectrometry 9 (1982), S. 186-190 
    Details
    ISSN: 0306-042X
    Keywords: Chemistry ; Analytical Chemistry and Spectroscopy
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: A gas chromatographic mass spectrometric assay using selected ion monitoring is described which permits the determination of trifluoperazine in plasma at concentrations ranging from 0.078 to 5.0 ng ml-1. It relies on the extraction of trifluoperazine and the internal standard, prochlorperazine or the tetradeuterated analogue of trifluoperazine, form basified plasma with a n-pentane/2-propanol solvent mixture. Following the evaporation of organic solvent, the residue is reconstituted in a small volume of methanol. Suitable aliquots were anlysed by the combined technique of gas chromatography/electron impact mass spectrometry with a data system. The described procedure is specific and enables the quantitation of 78 pg ml-1 of the drug with a coefficient of variation 〈7%. The method has demonstrated sufficient sensitivity to permit pharmacokinetic and bioavailability studies after a single 5 mg oral dose.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bms.1200090503
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    Paper (German National Licenses)
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  • 6
    Electronic Resource
    Electronic Resource
    A finite element analogue of the modified Rayleigh-Ritz method for vibration problems (1972)
    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 5 (1972), S. 163-169 
    Details
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: In this paper finite element method for natural vibration problems, based on a modified Rayleigh-Ritz method is presented. Numerical results of a few simple examples indicate that the method is promising.
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/nme.1620050203
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  • 7
    Electronic Resource
    Electronic Resource
    Computer program for solution of large, sparse, unsymmetric systems of linear equations (1977)
    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 11 (1977), S. 1251-1259 
    Details
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: A computer program is presented for in-core solution of a large, sparse, unsymmetric, unbanded system of linear equations. The program employs two partially packed arrays (one for storing non-zero elements, and one for column identifications). Used as the pivotal row is the row with the minimum number of non-zero elements. To avoid instability, the pivot is the largest absolute element in the pivotal row. The method was tested on a system of equations encountered in field application of the three-dimensional Galerkin finite element solution of flow and mass transport through porous media. Performance is compared with that of available alternatives.
    Additional Material: 3 Tab.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/nme.1620110806
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  • 8
    Electronic Resource
    Electronic Resource
    Node Reordering algorithms for water network analysis (1978)
    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 12 (1978), S. 1241-1259 
    Details
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: The node Admittance matrices for Water Networks are normally sparse and symmetrical. Advantage may be taken of these properties to reduce the amount of computational time and memory required to solve them. Two node reordering algorithms and an economic system of storing input data are described. The node reordering algorithms minimize the number of fill-ins which would occur in the solution of the node admittance matrix. Only non-zero elements are stored and operated on. Subroutines for the node reordering algorithms and storge of input data, written in FORTRAN IV are included.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/nme.1620120804
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  • 9
    Electronic Resource
    Electronic Resource
    Solution of water networks by sparse matrix methods (1978)
    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 12 (1978), S. 1261-1277 
    Details
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: A computer program for Water Network analysis has been developed usingy sparse matrix techniques for solution. The method used is the successive linearization method where the set of equations [Q]=[Y][H] is solved. In the process, two subroutines for node reordering to preserve sparsity of the Jacobian matrix and a subroutine for solving the matrix by the Doolittle method are included. The method is user oriented and requires a minimum amount of input data and effort. Networks of up to 200 nodes and 300 pipes may be analysed on an IBM 1130 computer with 16K words of memory.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/nme.1620120805
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  • 10
    Electronic Resource
    Electronic Resource
    Letters to the editor (1978)
    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 12 (1978), S. 1772-1773 
    Details
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/nme.1620121114
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