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  • 1
    Electronic Resource
    Electronic Resource
    155. Korrektureingriffe am oberen Sprunggelenk (1978)
    Springer
    Langenbeck's archives of surgery 347 (1978), S. 651-651 
    Details
    ISSN: 1435-2451
    Keywords: Ankle joint ; corrective procedures ; Sprunggelenksfrakturen ; Fehlstellungen ; Korrekturmöglichkeiten
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Im Rahmen einer AO-Sammelarbeit wurden aus 6 Kliniken Deutschlands insgesamt 135 Korrektureingriffe am oberen Sprunggelenk der Jahre 1962–1974 ausgewertet. Es wird über Indikationsstellung, Komplikationen und Behandlungsergebnisse berichtet. Die besten Ergebnisse wurden each Korrektureingriffen bei ursprünglichen Luxationsfrakturen vom Typ A und B erreicht. Hier beschränkten sich die Eingriffe vorwiegend auf die Stabilisierung einer Außen- oder Innenknöchelpseudarthrose. Bei Luxationsfrakturen vom Typ C überwiegen die Verängerungsosteotomien der Fibula — hier konnten in 43 % der Fälle gute Spätergebnisse erreicht wurden.
    Notes: Summary A total of 135 corrective procedures on the ankle joint performed in 6 German hospitals from 1962 to 1974 is reviewed. Indications, results of treatment, and complications are reported. Best results were achieved with corrective procedures following dislocation fractures of Type A or B. In most cases of this category, non-union of the lateral or medial ankle required stabilization. For old dislocation fractures of Type C, elongation osteotomy of the fibula was performed most frequently. Good longterm results were obtained in 43 % of cases.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01579448
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Factors affecting Agrobacterium tumefaciens-mediated transformation of peppermint (2000)
    Springer
    Plant cell reports 19 (2000), S. 304-310 
    Details
    ISSN: 1432-203X
    Keywords: Key words Peppermint ; Transformation ; Co-cultivation ; Coconut water ; Selection
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Substantial improvement in peppermint (Mentha x piperita L. var. Black Mitcham) genetic transformation has been achieved so that the frequency of transgenic plants regenerated (percent of leaf explants that produced transformed plants) was 20-fold greater than with the original protocol. Essential modifications were made to conditions for Agrobacterium tumefaciens co-cultivation that enhanced infection, and for selection of transformed cells and propagules during regeneration. A systematic evaluation of co-cultivation parameters established that deletion of coconut water from the co-cultivation medium resulted in substantially increased transient β-Glucuronidase (GUS) activity, in both the frequency of explants expressing gusA and the number of GUS foci per explant (〉700 explants). Co-cultivation on a tobacco cell feeder layer also enhanced A. tumefaciens infection. Enhanced transformation efficiencies were further facilitated by increased selection pressure mediated by higher concentrations of kanamycin in the medium during shoot induction, regeneration, and rooting: from 20 to 50 mg/l in shoot induction/regeneration medium and from 15 to 30 mg/l in rooting medium. Raising the concentration of kanamycin in media substantially lowered the number of "escapes" without significant reduction in plant regeneration. These modifications to the protocol yielded an average transformation frequency of about 20% (〉2000 explants) based on expression of GUS activity or the tobacco antifungal protein, osmotin, in transgenic plants. Genetic transformation of peppermint has been enhanced to the extent that biotechnology is a viable alternative to plant breeding and clonal selection for improvement of this crop.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050017
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Transgenic peppermint (Mentha×piperita L.) plants obtained by cocultivation with Agrobacterium tumefaciens (1998)
    Springer
    Plant cell reports 17 (1998), S. 165-171 
    Details
    ISSN: 1432-203X
    Keywords: Key words Peppermint ; Transformation ; Agrobacterium ; GUS ; Transgenic
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The first transgenic peppermint (Mentha×piperita L. cultivar Black Mitcham) plants have been obtained by Agrobacterium-mediated transformation by cocultivation with morphogenically responsive leaf explants. Basal leaf explants with petioles, from leaves closest to the apex of in-vitro-culture-maintained shoots (5 cm), exhibited optimal shoot organogenetic responsiveness on medium supplemented with thidiazuron (8.4 µm). Shoot formation occurred at sites of excision on the leaf blade and petiole either directly from cells of the explant or via a primary callus. Analyses of transient GUS activity data indicated that DNA delivery by microprojectile bombardment was more effective than Agrobacterium infection. However, no transgenic plants were obtained from over 22,000 leaf explants after particle bombardment. Cocultivation of leaf explants with Agrobacterium strain EHA 105 and kanamycin selection produced transgenic plants. Greater transient and stable -glucuronidase (GUS) activities were detected in explants or propagules transformed with the construct where gusA was driven by the pBISN1 promoter rather than a CaMV 35S promoter. Eight plants were subsequently regenerated and verified as transgenic based on detection of the nptII transgene by PCR and Southern blot analyses. The Southern analyses indicated that the plants were derived from eight unique transformation events. All transgenic plants appeared morphologically normal. Analyses of GUS activities in leaves sampled from different portions of these transgenic plants, 10 months after transfer to the greenhouse, indicated that six out of the eight original regenerants were uniformly transformed, i.e., did not exhibit chimeric sectors.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050372
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    67. Die posttraumatische und postoperative Frühinfektion nach offenen und geschlossenen Knochenbrüchen — Einführung in das Thema (1985)
    Springer
    Langenbeck's archives of surgery 366 (1985), S. 363-363 
    Details
    ISSN: 1435-2451
    Keywords: Posttraumatic and postoperative infection ; Surgical measures and concomitant therapeutic suggestions ; Posttraumatische und postoperative Infektion ; Behandlungsmaßnahmen ; Empfehlungen
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Die Infektion nach offenen Knochenbrüchen und nach operativen Eingriffen mit Osteosynthese primär geschlossener Frakturen läßt sich trotz aller Bemühungen und Fortschritte der modernen Medizin bis heute nicht sicher vermeiden. Auch weiterhin schwebt diese Komplikation wie ein Damoklesschwert über den verletzten Patienten. Frühzeitiges Erkennen und konsequente chirurgische Therapie lassen neben den bekannten Vorsorgemaßnahmen in vielen Fällen bleibende Schäden und ein mitunter langes Krankenlager verhüten. In den folgenden Referaten werden alle wichtigen therapeutischen Gesichtspunkte besprochen und einschlägige Beispiele demonstriert. Abschließend werden in einem Rundgespräch und einer Aussprache mit dem Auditorium offene Fragen diskutiert und weitere Erfahrungen mitgeteilt. Es wird versucht am Ende einen Katalog von Behandlungsempfehlungen zusammenzustellen.
    Notes: Summary In spite of all endeavours and progresses of modern medicine we are still unable to securely prevent an infection. This complication furthermore will stay like a “Damocles sword” above the injured patient. Early diagnosis and immediate consequent surgical therapy besides all preventive measures, however, may keep the patient off irreversible disabilities and long-lasting illness. The different lectures will report on the present knowledge and inform the audience about all necessary therapeutic steps. In a final panel session open questions will be discussed and therapeutic suggestions will be given.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01836666
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    Paper (German National Licenses)
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