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  • 1
    Electronic Resource
    Electronic Resource
    Trennung der intestinalen β-Galactosidasen beim lactose-intoleranten Erwachsenen durch Ultrazentrifugation im Dichtegradienten (1972)
    Springer
    Journal of molecular medicine 50 (1972), S. 260-261 
    Details
    ISSN: 1432-1440
    Keywords: Lactose intolerance ; intestinalβ-Galactosidases ; ultracentrifugation ; Lactose-Intoleranz ; intestinaleβ-Galactosidasen ; Ultrazentrifugation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Bei 11 erwachsenen Versuchspersonen mit Lactose-Intoleranz konnten bei der Trennung der intestinalenβ-Galactosidasen im Dichtegradienten die „neutrale“ Bürstensaum-Lactase und die „saure“ lysosomale Lactase nachgewiesen werden. Im Vergleich zu lactose-toleranten Erwachsenen war die Bürstensaum-Lactase stark vermindert. Die Hetero-β-Galactosidase, die bei lactose-toleranten Erwachsenen als langsam sedimentierendes Enzym nachweisbar ist, fehlte bei allen 11 Versuchspersonen.
    Notes: Summary In 11 adult probands with lactose intolerance the intestinalβ-galactosidases were separated in a density gradient by ultracentrifugation. The “neutral” brush border lactase and the “acid” lysosomal lactase were identified in the biopsy material of all 11 subjects. In comparison with lactosetolerant adults the brush border lactase was markedly diminished. The hetero-β-galactosidase which is present as a slowly sedimenting enzyme in lactose-tolerant adults, was absent in all of the 11 subjects.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01486533
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  • 2
    Electronic Resource
    Electronic Resource
    Trennung der intestinalen β-Galactosidasen beim lactose-toleranten Erwachsenen durch Ultrazentrifugation im Dichtegradienten (1972)
    Springer
    Journal of molecular medicine 50 (1972), S. 258-259 
    Details
    ISSN: 1432-1440
    Keywords: Lactose tolerance ; intestinalβ-Galactosidases ; ultracentrifugation ; Lactose-Toleranz ; intestinaleβ-Galactosidasen ; Ultrazentrifugation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Die intestinalenβ-Galactosidasen von 4 lactose-toleranten, erwachsenen Mitteleuropäern wurden im Saugbiopsie-Gewebe nach Solubilisierung mit Triton X-100 in einem linearen Mannitol-Gradienten (5–20%) auf der Ultrazentrifuge bei 4°C und 44000 U/min getrennt. Bei 12stündiger Zentrifugation fanden sich 3 Fraktionen, von denen die beiden schneller sedimenticrenden Lactose spalten. Alle 3 Fraktionen hydrolysieren p-Nitrophenyl-β-Galactosid. Die 3 isoliertenβ-Galactosidasen entsprechen wahrscheinlich der „neutralen“ Bürstensaum-Lactase, der „sauren“ lysosomalen Lactase und einer cytoplasmatischen Hetero-β-Galactosidase.
    Notes: Summary The intestinalβ-galactosidases in suction biopsy material of 4 lactose-tolerant adult Europeans were separated after solubilization with Triton X-100 in a linear 5–20% mannitol gradient by ultracentrifugation at 4°C and 44000 rpm. After a centrifugation time of 12 h 3 fractions were identified. The 2 fractions which sedimented more rapidly were active with lactose as substrate; all 3 fractions hydrolized p-nitrophenyl-β-galactoside. The 3 isolatedβ-galactosidases probably correspond to the “neutral” brush border lactase, the “acid” lysosomal lactase, and a cytoplasmatic hetero-β-galactosidase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01486532
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Antiretikulin-Antikörper und präcipitierende Antikörper gegen Nahrungsmittelproteine im Serum von Kindern mit Cöliakie (1976)
    Springer
    European journal of pediatrics 121 (1976), S. 215-226 
    Details
    ISSN: 1432-1076
    Keywords: Coeliac disease ; Antireticulin antibodies ; Precipitating antibodies to wheat flour ; Precipitating antibodies to cow's milk
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Antiretikulin-Antikörper im Serum wurden mit der Methode der indirekten Immunfluorescenz an frischen Rattennierenschnitten bei 11/13 Kindern (85%) mit florider Cöliakie, 7/17 Kindern (41%) mit klinisch und biochemisch stummem, bioptisch nachgewiesenem Cöliakie-Rezidiv und 0/16 Kindern mit behandelter Cöliakie nachgewiesen. Präcipitierende Antikörper im Serum gegen Weizenmehl bzw. Kuhmilch wurden mit Hilfe einer Kombination aus Elektrophorese und Immundiffusion bei 3/13 Kindern (23%) bzw. 2/13 Kindern (15%) mit florider Cöliakie und bei 1/16 Kindern (6%) mit behandelter Cöliakie gefunden. Bei den 17 Kindern mit stummem Cöliakie-Rezidiv fiel die Untersuchung auf präcipitierende Antikörper negativ aus. 40 Kontrollkinder wiesen weder Antiretikulin-Antikörper noch präcipitierende Antikörper gegen Weizenmehl oder Kuhmilch auf. Die Bedeutung des Antiretikulin-Antikörper-Nachweises für Screening-Untersuchungen und für die Verlaufskontrolle wird diskutiert.
    Notes: Summary Sera from 41 children suffering with histologically proven coeliac disease and from 40 healthy control children were investigated for the presence of antireticulin antibodies and precipitating antibodies to a watery extract of wheat flour and to cow's milk. Antireticulin antibodies were demonstrated by means of indirect immunofluorescence using sections of fresh rat kidney as substrat. For the detection of precipitating antibodies a combination of electrophoresis and immunodiffusion was used. Serum antireticulin antibodies were found in 11/13 children (85%) with active coeliac disease, in 7/17 children (41%) with clinically and biochemically silent coeliac relapse and in 0/16 children with treated coeliac disease. Serum precipitating antibodies to wheat flour and cow's milk were found respectively in 3/13 children (23%) and 2/13 children (15%) with active coeliac disease and in 1/16 children (6%) with treated coeliac disease. Precipitating antibodies could not be detected in the sera of 17 patients with silent relapse of coeliac disease. In the sera of 40 controls neither antireticulin nor precipitating antibodies were detectable. The presence of antireticulin antibodies in serum did not correspond to the presence of serum precipitins to wheat flour and cow's milk. The significance of serum antireticulin antibodies for sereening investigations and for follow-up studies is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00445484
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    Paper (German National Licenses)
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