Library

feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    ISSN: 1432-069X
    Keywords: Keywords HSP70 ; Human melanoma cells ; Ultraviolet B ; Apoptosis ; Caspase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The heat shock response is a highly conserved reaction common to all cells and organisms. It has been reported that hyperthermic treatment can induce the expression of the heat shock protein (HSP) and can protect cells from ultraviolet (UV) B radiation. In this study, we evaluated the effects of induced HSP70 on resistance to UV radiation. G361 amelanotic human melanoma cells were irradiated with increasing doses of UVB. UVB irradiation caused apoptotic cell death in these cells. Following transfection with MFG.hsp70.puro plasmid, the expression of HSP70 was determined. Compared to control vector-transfected cells, hsp70-transfected cells showed significantly elevated levels of HSP70 and were highly resistant to UVB irradiation. In order to investigate the effects of HSP70 on the apoptotic pathway, the changes in caspase-3 and PARP were analyzed. Following UVB irradiation, activation of caspase-3 and cleavage of PARP were observed in control vector-transfected cells, and the changes in these molecules were inhibited in the hsp70-transfected cells. These results suggest that UVB-induced apoptosis of melanoma cells is accompanied by caspase-3 activation and PARP cleavage, which can be prevented by an overexpression of HSP70.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Rheologica acta 28 (1989), S. 13-24 
    ISSN: 1435-1528
    Keywords: One-modeGiesekus model ; mobility parameter ; Weissenberg number ; Couette flow ; Poiseuille flow
    Source: Springer Online Journal Archives 1860-2000
    Topics: Chemistry and Pharmacology , Physics
    Notes: Abstract Analytical solutions for the plane Couette flow and the plane Poiseuille flow of the one-mode Giesekus fluid without any retardation time have been obtained by considering the domain of definition for each of the two branch solutions which arise due to the presence of the quadratic stress terms in the constitutive equations. For each fixed value of the mobility parametera, the limiting value of the Weissenberg number for the upper branch solution, i.e., the physically realistic solution is determined in terms of the corresponding dimensionless shear stress for the plane Couette flow and in terms of the corresponding dimensionless pressure gradient for the plane Poiseuille flow. In the case of the plane Couette flow, it is shown that fora falling in the range 0≤a≤1/2 only the physically realistic solution exists while for 1/2〈a ≤1 a nonphysical solution coexists with the realistic one. In the case of the plane Poiseuille flow, it is shown that the non-physical solution cannot even exist around the center plane of the channel, and the effects of the mobility parameter and the dimensionless pressure gradient on the flow variables are investigated. Possible extensions of the present approach to other steady simple shear flows with and without the introduction of the retardation time are also discussed.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...