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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Urological research 12 (1984), S. 111-113 
    ISSN: 1434-0879
    Keywords: Computed tomography ; Attenuation value ; Composition of renal calculi ; In vitro
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The attenuation values of computed tomography were studied in 50 recovered renal calculi of more than 10 mm in diameter, in the hope of determining the composition of calculi in situ. The attenuation value of various calculi (mean±s.d.) in a 5 mm slice with a maximal rectangular region of interest was as follow (Hounsfield units); mixed calcium oxalate and phosphate 1,555±193 (n=22), magnesium ammonium phosphate 1,285±284 (n=18), calcium oxalate 1,690 (n=1), calcium phosphate 1,400 (n=2), cystine 757±114 (n=5) and uric acid 480 (n=2). Attenuation values ranging from 500 to 1,600 overlapped for various calculi, except those composed of uric acid calculi. There was no correlation between the attenuation value and the mineral content such as calcium or magnesium per unit weight of calculus. The mineral content per unit volume seemed to be attributable to the attenuation value. From the present results we conclude that the determination of calculous composition by the attenuation value is possible only for oxalate calculi of more than 1,600 and uric acid calculi of less than 500 attenuation value, provided that the proper CT slice location, the region of interest and the appropriate calculus size can be determined.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1434-0879
    Keywords: Renal stone ; Cell culture models ; MDCK cell line ; Calcium phosphate
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Cell culture models of calcium phosphate renal stone formation were established using the MDCK cell line. Renal microliths were detected within pseudocysts in three-dimensional soft agar cultures, and were also observed in the basal region of cells lining the cell sheet, and immediately beneath domes or blisters in monolayers and collagen gel cultures. Light and scanning electron microscopy indicated that these microliths had a similar lamellated and spherical appearance to those in humans. These microliths were first detected microscopically after 21 days of culture, and were found to be composed of calcium phosphate by X-ray and microinfrared spectroscopic analyses. These culture models may provide a powerful new tool to study the pathogenesis of renal stone diseases and/or calcium phosphate stone formation in humans and animals.
    Type of Medium: Electronic Resource
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