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  • General Chemistry  (45)
  • Chemical Engineering  (11)
  • NMR  (7)
  • Autoradiography  (6)
  • 1
    ISSN: 1432-0878
    Keywords: LH-cells ; Prolactin cells ; Immunocytochemistry ; Estrogen ; Autoradiography ; Guinea pig ; Hamster ; Gerbil
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Nuclear uptake and retention of3H-estradiol by luteinizing hormone (LH) and prolactin (PRL) cells was examined in three species of rodents (guinea pigs, hamsters and gerbils) using the combined techniques of immunocyto-chemistry and autoradiography. Castrated animals were injected with3H-estradiol and decapitated 1.5 h later. The pituitary glands were processed for thaw-mount autoradiography followed by conventional immunocytochemical staining for LH and PRL.3H-estradiol accumulated in more than 80% of the anterior pituitary cells in the gerbils, while only 33 and 22% of the cells accumulated3H-estradiol in the hamsters and guinea pigs, respectively. A varying percentage of immunoreactive LH and PRL cells in all three species were found also to contain binding sites for estradiol. Some LH and PRL cells in hamsters and guinea pigs and only some in PRL cells of gerbils were found to be devoid of grains. Quantitative analysis revealed that the number of grains per nucleus differed considerably from cell to cell. LH cells of guinea pigs accumulated much larger amounts of3H-estradiol than did the PRL cells, while the LH cells in the hamsters and gerbils accumulated only slightly more3H-estradiol than the PRL cells. These results confirm the previous observations in rats and baboons that demonstrated tremendous species differences in percentage of cells in the anterior pituitary gland that accumulated3H-estradiol. Also, these data suggest that there are functionally heterogeneous cell types among the LH and PRL cells in hamsters, guinea pigs and gerbils as has been previously demonstrated in rats and baboons.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    New York, NY : Wiley-Blackwell
    Proteins: Structure, Function, and Genetics 32 (1998), S. 334-349 
    ISSN: 0887-3585
    Keywords: antifungal ; thionin ; NMR ; structure ; scorpion ; toxins ; Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine
    Notes: The three-dimensional structure of the Sorghum bicolor seed protein γ-thionin SIα1 has been determined by 2D 1H nuclear magnetic resonance (NMR) spectroscopy. The secondary structure of this 47-residue antifungal protein with four disulphide bridges consists of a three-stranded antiparallel sheet and one helix. The helix is tethered to the sheet by two disulphide bridges which link two successive turns of the helix to alternate residues i, i + 2 in one strand. Possible binding sites for antifungal activity are discussed. The same fold has been observed previously in several scorpion toxins. Proteins 32:334-349, 1998. © 1998 Wiley-Liss, Inc.
    Additional Material: 10 Ill.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Bognor Regis [u.a.] : Wiley-Blackwell
    Journal of Polymer Science Part A: Polymer Chemistry 31 (1993), S. 2835-2838 
    ISSN: 0887-624X
    Keywords: NMR ; MAS ; silanes ; Ziegler-Natta ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: 29Si cross-polarisation NMR was used to study the interaction between silanes and MgCl2 when co-milled. Large changes are seen for the 29Si chemical shift of the silane between the silane in solution and in the solid complex. Interpretation of the differences in chemical shift are hindered by the absence of an adequate semi-empirical theory. The 29Si chemical shift tensors confirm a strong interaction between the silane and MgCl2. © 1993 John Wiley & Sons, Inc.
    Additional Material: 1 Ill.
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Bognor Regis [u.a.] : Wiley-Blackwell
    Journal of Polymer Science Part A: Polymer Chemistry 34 (1996), S. 971-984 
    ISSN: 0887-624X
    Keywords: radiation ; polyisobutylene ; NMR ; GC/MS ; Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology
    Notes: GC, GC/MS, GPC, and Solution NMR spectra were used to study the γ radiolysis of polyisobutylene (PIB) in vacuum to different total doses (0-900 kGy) and at different temperatures (77-423 K). NMR spectra show a large number of new resonances with relatively narrow line widths, and a variety of NMR techniques have been employed to determine and quantify the structures associated with these new resonances. Chemical shift assignments were made by comparison with those for small molecule model compounds and predictions based upon calculations according to several different schemes. Chain-end structures have been proposed that account well for the majority of the new resonances, all being the result of an initial chain scission reaction initiated by the radiation. These assignments support some previous proposals for the mechanism of radiation degradation of polyisobutylene and exclude others. For example, NMR provides no evidence for the formation of ethyl chain ends and some of the main chain unsaturated structures previously proposed. NMR also indicate that at higher doses the chain end products formed during initial stages undergo secondary reactions. GC/MS data show the formation of oligomers during irradiation, which may be due to a backbitting mechanism. © 1996 John Wiley & Sons, Inc.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
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  • 5
    Electronic Resource
    Electronic Resource
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 43 (1997), S. 1894-1903 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: The role of swelling clays in hindering the compressional dewatering characteristics of coal-mine tailings is examined. The effects of electrolyte concentration and ion exchange in improving the shear and compressional rheology are compared. Suspensions studies include actual mine tailings (thickener feed and thickener underflow) as well as synthetic clay dispersions made from clay collected from the coal seam. It was shown that the most important parameter in controlling the properties of the tailings suspension is “controlled” dispersion in the presence of a Ca2+ electrolyte concentration in excess of that required to (1) prevent initial swelling and (2) provide full cation exchange of the clay. Under these electrolyte conditions, complete delamination of the clay did not occur, and both the dewatering and handling characteristics of the resultant suspensions improved dramatically.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 43 (1997), S. 1171-1179 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A new theoretical approach is proposed for the yield stress of concentrated, flocculated particulate suspensions. Explicit cognizance is taken for the three-dimensional, mechanically rigid particle network held together by interparticle forces wherein the mean coordination number in the assemblage increases and the separation between the particles decreases with an increase in the volume fraction of the solid phase. The Rump-Molerus model relting isotropic normal stress and isotropic normal interparticle force in a bed of single-sized spheres is modified to incorporate the size distribution of particles and extended to the suspension network. The model estimates the yield stress as a function of solids loading for various kinds of size distribution and is in reasonable agreement with experimental data when the surface properties of the particle are held constant.
    Additional Material: 11 Ill.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 36 (1990), S. 127-131 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 38 (1992), S. 343-362 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A model is presented for a quantitative prediction of the transfer rates of momentum, heat and mass in turbulent pipe flow. In this so-called extended random surface renewal (ERSR) model, the tube wall is assumed to be covered by a mosaic of fluid elements of random age and laminar flow with unsteady profiles of axial velocity, temperature or concentration.Both the age distribution and the mean age of the fluid elements at the tube wall, predicted by the ERSR model, quantitatively agree with experimental results obtained from velocity signals measured with a laser-Doppler anemometer at 5·103≤Re≤43·103. The time-averaged radial profiles of the axial velocity, the temperature and the concentration in the wall region, and the heat- and mass-transfer coefficients derived with the ERSR model agree with empirical results presented in literature. Furthermore, the ERSR model provides a basis for explaining the Chilton-Colburn analogy.
    Additional Material: 14 Ill.
    Type of Medium: Electronic Resource
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  • 9
    Electronic Resource
    Electronic Resource
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 35 (1989), S. 1592-1601 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Various strategies for sequencing heterogeneous azeotropic distillations have been explored. The design and optimization of these systems by simulation-based methods is generally acknowledged to be a very difficult problem (Kingsley and Lucia, 1988). In this article, a simple and accurate design and optimization procedure has been developed. The method does not involve any recycle convergence loops and has been applied successfully to several test problems. Unlike the simulation-based methods, our method is robust to changes in the system optimization variables (e.g., reflux ratio in the azeotropic column).In order to demonstrate the method, we present design and optimization results for the dehydration of ethanol using a benzene entrainer. Our optimal designs consume significantly less energy than state-of-the-art designs reported in the literature.
    Additional Material: 16 Ill.
    Type of Medium: Electronic Resource
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  • 10
    Electronic Resource
    Electronic Resource
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 35 (1989), S. 1380-1384 
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 2 Ill.
    Type of Medium: Electronic Resource
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