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  • 1
    Electronic Resource
    Electronic Resource
    Viability and recovery of frozen-thawed human islets and in vivo quality control by xenotransplantation (1999)
    Springer
    Journal of molecular medicine 77 (1999), S. 172-174 
    Details
    ISSN: 1432-1440
    Keywords: Key words Islets of Langerhans ; Cryopreservation ; Vitrification ; Transplantation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Cryopreservation of islets of Langerhans offers advantages for the transplantation into diabetic patients. In this study two different methods of cryopreservation were compared with respect to islet viability and recovery after cryostorage. It was also investigated whether human islet survival in mice was affected by cryopreservation. Aliquots of human islets were cryopreserved conventionally or vitrified, respectively. After rapid thawing, islet viability and islet equivalent (IEQ) recovery rate were determined. Aliquots of freshly isolated or conventionally cryopreserved islets were transplanted beneath the kidney capsule of non-diabetic C57BL/6 mice. After three days renal insulin content was determined. Islet cell viability was 17.3±8.0% for vitrified and 51.8±3.0% for conventionally cryopreserved islets; the recovery rate was 84.8±12.2% and 92.8±12.4%, respectively. Insulin recovery after transplantation was 25.6±7.3% for fresh and 24.1±7.4% for cryopreserved islets. This study suggests that the conventional method of cryopreservation is superior to vitrification with respect to islet viability after thawing. We found no significant difference between fresh and cryopreserved islets with respect to insulin recovery after transplantation into mice.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001090050330
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Evidence for a significant correlation of donor pancreas morphology and the yield of isolated purified human islets (1999)
    Springer
    Journal of molecular medicine 77 (1999), S. 87-89 
    Details
    ISSN: 1432-1440
    Keywords: Key Words Pancreas ; Islets of Langerhans ; Islet isolation ; Pancreas morphology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract In clinical islet transplantation to patients with type 1 diabetes mellitus, the number of isolated and purified islet has been identified as a key determinant for functional success of the islet graft [1]. With improved isolation methods based on the original procedure published by Ricordi et al. [2] yield and function of isolated islets were considerably enhanced. However, there is still a large variance in the number, purity, viability and secretory capacity of islets isolated from brain-dead human donor pancreata, significantly hampering utilization of human islet preparations derived from a single donor for one diabetic recipient. The reasons for the limited success in islet isolation and purification have not been clarified in detail yet. Recent studies have indicated, that donor preconditions, and a number of technical factors during organ procurement and the islet isolation process itself are critical to successful islet isolation [3, 4]. This study aimed at identifying distinct morphological and histopathological characteristics of the donor pancreas as determinants for the outcome of human islet isolation and purification.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001090050308
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Critical islet mass for successful porcine islet autotrasplantation (1999)
    Springer
    Journal of molecular medicine 77 (1999), S. 126-129 
    Details
    ISSN: 1432-1440
    Keywords: Key words Pancreatectomy ; Porcine islets ; Autotransplantation ; Glucose tolerance test
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A major reason for the failure of clinical islet transplantations may be a limited islet mass. The aim of this study was to determine the critical islet mass necessary for normalization of glucose metabolism in a porcine model. Diabetes was induced by total pancreatectomy. The splenic lobe of the pancreas was intraductally distended with UW-solution containing 2.67–3.33 mg/ml collagenase, and the distended pancreas was digested in a continuous digestion filtration device. The islets were purified on a isoosmotic Ficoll-sodium-diatrizoate gradient. The survival period of the diabetic recipients in group 2 and 3 receiving, respectively, a low (2.14±0.39 µL/kg body weight) and a high (4.99±0.83 µL/kg body weight) islet mass was significantly prolonged compared to that of diabetic recipients in group 1 receiving no islet transplantation. However, the survival period of the recipients in group 2 was not significantly different to that in group 3. Three recipients of an islet mass of 〉5 µl/kg body weight became normoglycemic (fasting blood glucose 〈100 mg/dl) for more than two months. Furthermore, the glucose and insulin release reactions to the glucose challenge were comparable to that before pancreatectomy. Contrarily, another five diabetic recipients of an islet mass of 〈4 µL/kg body weight became a fasting blood glucose level of 〈200 mg/dl. The glucose and insulin release reactions to the glucose challenge were improved only, but not normalized compared to that before pancreatectomy. The data presented in this study demonstrate that metabolic normalization in pancreatectomized diabetic minipigs can be established by autotransplantation of an islet mass of 〉5 µl/kg body weight.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001090050319
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    Paper (German National Licenses)
    Fulltext
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