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  • 1
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Biochimica et Biophysica Acta (BBA)/Biomembranes 554 (1979), S. 1-22 
    ISSN: 0005-2736
    Keywords: (Electron microscopy) ; Membrane protein ; Membrane structure ; Phase separation ; Picture analysis
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Medicine , Physics
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    [s.l.] : Macmillan Magazines Ltd.
    Nature 400 (1999), S. 544-547 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The observation that clusters of neutral H2O () or SO2 (ref. 5) molecules, on impact with essentially any solid surface, can decay efficiently into positively and negatively charged fragments has defied explanation, not least because the kinetic energy ...
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 0014-5793
    Keywords: Anoxia ; Heart cell ; K"A"T"P channel ; Patch clamp
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 4
    Electronic Resource
    Electronic Resource
    Amsterdam : Elsevier
    Biochemical and Biophysical Research Communications 84 (1978), S. 508-514 
    ISSN: 0006-291X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 0196-9781
    Keywords: ''Incretin'' deficiency ; Conscious Beagle dog ; Entero-insular axis of insulin ; Oral and intravenous glucose administration ; Pancreas atrophy ; Pancreatic duct occlusion ; Somatostatin ; VIP ; VIP is no ''incretin''
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Chemistry and Pharmacology
    Type of Medium: Electronic Resource
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  • 6
    Electronic Resource
    Electronic Resource
    Springer
    World journal of surgery 5 (1981), S. 269-273 
    ISSN: 1432-2323
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé L'article analyse 117 duodénopancréatectomies pour pancréatite chronique, 49 partielles et 68 totales. La mortalité opératoire a été 8.2% pour les résections partielles et de 20.6% pour les totales. Les opérés ont été suivis pendant 6 1/2 années: 76% des résections partielles et 63% des totales ont continué à boire autant d'alcool qu'avant l'intervention. Avant pancréatectomie totale, 42% des malades étaient diabétiques; après cette opération, tous avaient un diabète, difficile à équilibrer à l'insuline dans 75% des cas qui ont souffert d'épisodes répétés de choc hypoglycémique. La mortalité tardive a été de 20.4% après duodénopancréatectomie partielle et de 19.1% après résection totale. Après pancréatectomie totale, 50% des décès tardifs ont été dus aux accidents d'hypoglycémie. A la fin de la période de follow-up, la survie du groupe pancréatectomie totale est de 11% inférieure à celle des résections partielles. La pancréactectomie totale n'est justifiée que chez les malades qui ont, avant l'intervention, un diabète exigeant l'administration d'insuline. Nous décrivons une nouvelle technique qui réalise, après résection de la tête du pancréas, une occlusion des canaux pancréatiques par injection d'une solution d'acides aminés à prise rapide, ce qui entraîne après quelques semaines une atrophie du pancréas exocrine. Cette technique a été utilisée chez 39 malades, avec 2.5% de décès et 7.6% de complications postopératoires. Nous estimons que cette nouvelle technique peut réduire nettement le risque opératoire de la duodénopancréatectomie partielle pour pancréatite chronique et améliorer les résultats à long terme.
    Notes: Abstract This is a report of 117 pancreaticoduodenectomies performed for chronic pancreatitis, of which 49 were partial and 68 were total. The operative mortality rate of partial pancreaticoduodenectomy was 8.2% and of total pancreatectomy was 20.6%. During a follow-up period of 6 1/2 years, 76% and 63% of the surgical patients, respectively, continued to drink alcohol as heavily as before. Prior to total pancreatectomy, only 42% of the patients had diabetes. After total extirpation of the organ, all had diabetes and 75% were very difficult to stabilize with insulin, experiencing repeated episodes of hypoglycemic shock. The additional late mortality rate was 20.4% following partial pancreaticoduodenectomy and 19.1% after total resection. After total pancreatectomy, 50% of the late deaths were due to hypoglycemia. After total pancreatectomy, 11% fewer patients were still alive at the end of the follow-up period than after partial pancreaticoduodenectomy. Total pancreatectomy is justified only in patients who already have diabetes requiring insulin. A new technique is described in which, following resection of the head of the pancreas, the duct system is occluded by injection of a rapidly hardening amino acid solution, leading to atrophy of the excretory pancreas within a few weeks. This procedure has been carried out in 39 patients with a mortality rate of 2.5% and postoperative complications in 7.6%. We believe that the immediate risk of partial pancreaticoduodenectomy in chronic pancreatitis can be decreased markedly and the late results improved by this new technique.
    Type of Medium: Electronic Resource
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  • 7
    Electronic Resource
    Electronic Resource
    Springer
    World journal of surgery 5 (1981), S. 721-724 
    ISSN: 1432-2323
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé Le cancer gastrique précoce est défini par un carcinome dont la localisation est limitée à la muqueuse et la sous muqueuse. En fonction de cette définition on peut considérer 2 types de carcinome le cancer muqueux et le cancer sous muqueux. L'existence ou non de métastases ganglionnaires n'est pas à prendre en considération dans la définition. Les progrès les plus marqués dans la détection du cancer de l'estomac ont été faits au Japon depuis 1960. Dès maintenant, près de 30% de tous les malades qui présentènt un cancer de l'estomac sont traités à un stade précoce dans ce pays. Cependant, en Europe, des progrès ont été également atteints. Avant la pratique de l'endoscopie le taux des cancers traités à un stade précoce était inférieur à 3%. Depuis lors il s'est élevé à 6% dans la plupart des pays européens et a même atteint 16% dans notre formation hospitalière. Le diagnostic de cancer précoce est établi le plus souvent par endoscopie. Le taux de fiabilité de la gastroscopie est d'environ 92% (Rösch) et atteint même 98% lorsque l'endoscopie est compétée par la biopsie. En raison du fait que le cancer de l'estomac est multifocal dans plus de 10%, l'endoscopiste doit systématiquement rechercher une seconde ou une troisième localisation au niveau de la totalité de l'estomac. Pour établir fermement le diagnostic de cancer en présence d'une ulcération, de multiples prélèvements doivent être effectués aussi bien au niveau du fond que des bords de l'ulcération. Les lésions ulcéreuses malignes pouvant régresser de temps en temps et même cicatrisées les prélèvements doivent être faits en présence de lésions cicatricielles. Lorsque les lésions revêtent un type polypöide, une large excision est nécessaire pour établir le diagnostic. Dans quelques cas choisie, elle représente une méthode thérapeutique.
    Notes: Abstract In the last 10 years, 130 patients with early gastric carcinoma were operated on at the Surgical University Clinic of Erlangen. The ulcerous type was the most frequent (85%) and had the best prognosis with a 5-year survival rate of 87% excluding operative mortality, and 100% when agecorrected.
    Type of Medium: Electronic Resource
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  • 8
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 101 (2000), S. 1001-1007 
    ISSN: 1432-2242
    Keywords: Key words Chromosome identification ; Physical mapping ; FISH ; BAC ; Molecular cytogenetics ; Potato
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  Reliable and easy to use techniques for chromosome identification are critical for many aspects of cytogenetic research. Unfortunately, such techniques are not available in many plant species, especially those with a large number of small chromosomes. Here we demonstrate that fluorescence in situ hybridization (FISH) signals derived from bacterial artificial chromosomes (BACs) can be used as chromosome-specific cytogenetic DNA markers for chromosome identification in potato. We screened a potato BAC library using genetically mapped restriction fragment length polymorphism markers as probes. The identified BAC clones were then labeled as probes for FISH analysis. A set of 12 chromosome-specific BAC clones were isolated and the FISH signals derived from these BAC clones serve as convenient and reliable cytological markers for potato chromosome identification. We mapped the 5S rRNA genes, the 45S rRNA genes, and a potato late blight resistance gene to three specific potato chromosomes using the chromosome-specific BAC clones.
    Type of Medium: Electronic Resource
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  • 9
    ISSN: 1432-2242
    Keywords: Solanum tuberosum ; P. infestans ; Resistance ; Dihaploid induction ; RFLP mapping
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract In potato, 11 resistance alleles (R1–R11) are known which confer race-specific resistance to the fungus Phytophthora infestans. R1 has been mapped previously to potato chromosome V and R3 to chromosome XI. Here we report on the localization of the R6 and R7 alleles on the genetic map of potato. Differential resistant strains of tetraploid Solanum tuberosum, clones MaR6 and MaR7, were used as parental plants for the parthenogenetic induction and selection of diploid genotypes containing the R6 or the R7 resistance allele to P. infestans. One resistant dihaploid from MaR7 could be used directly as a parent to produce diploid F1 progeny suitable for phenotypic and RFLP analysis. MaR6 did not produce useful dihaploids directly. After crossing MaR6 with a tetraploid susceptible genotype, resistant F1 clones were selected. The resistant genotypes were then used as parents for the induction of dihaploids. Six dihaploids bearing R6 were identified that could be crossed with a diploid susceptible genotype. Two diploid F1 populations, segregating for R6 and R7, respectively, were analysed with RFLP markers known to be linked with previously identified R genes. Markers linked with R3 were found also to be linked with R6 and R7. The resistance alleles R6 and R7 mapped to a similar distal position on chromosome XI as the R3 allele.
    Type of Medium: Electronic Resource
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  • 10
    ISSN: 1432-2242
    Keywords: Key words Plants ; Disease resistance ; Host-pathogen interaction ; Synchytriumendobioticum ; Potato
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The fungus Synchytrium endobioticum, the causal agent of potato wart disease, is subject to world-wide quarKantine regulations due to the production of persistent resting spores and lack of effective chemical control measures. The selection of Synchytrium-resistant potato cultivars may be facilitated by using markers closely linked with a resistance gene or by transferring a cloned gene for resistance into susceptible cultivars. Sen1, a gene for resistance to Synchytrium endobioticum race 1, was localized on potato chromosome XI in a genomic region which is related to the tobacco genome segment harbouring the N gene for resistance to TMV. Using N as probe, we isolated homologous cDNA clones from a Synchytrium-resistant potato line. The N-homologous sequences of potato identified by RFLP mapping a family of resistance gene-like sequences closely linked with the Sen1 locus. Sequence analysis of two full-length N-homologous cDNA clones revealed the presence of structural domains associated with resistance gene function. One clone (Nl-25) encodes a polypeptide of 61 kDa and harbours a Toll-interleukin like region (TIR) and a putative nucleotide binding site (NBS). The other clone (Nl-27) encodes a polypeptide of 95 kDa and harbours besides the TIR and NBS domains five imperfect leucine-rich repeats (LRRs). Both clones have at their amino terminus a conserved stretch of serine residues that was also found in the N gene, the RPP5 gene from Arabidopsis thaliana and several other resistance gene homologues, suggesting a function in the resistance response. Cloning of the disease resistance locus based on map position and the establishment of PCR-based marker assays to assist selection of wart resistant potato genotypes are discussed.
    Type of Medium: Electronic Resource
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