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  • Bean internodes  (1)
  • Candida amapae  (1)
  • Glioma  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Acid hydrolase and cytochrome oxidase activities in nitrosourea induced tumors of the nervous system (1977)
    Springer
    Acta neuropathologica 39 (1977), S. 13-23 
    Details
    ISSN: 1432-0533
    Keywords: Nitrosourea ; Glioma ; Schwannoma ; Hydrolases ; Oxidase
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Nitrosourea induced tumors of the nervous system in rats have proven useful for biochemical studies combined with morphological approaches. The pattern of enzyme activities for acid hydrolases and cytochrome oxidase resemble those previously observed in spontaneous nervous system tumors of man. The activities of 4 acid hydrolases were generally high in the gliomas. This could not be attributed solely to zones of regression or necrosis but was a general characteristic of the neoplasms. The activities were predominantly particulate and most likely lysosomal in localization. In schwannomas a similar increase in hydrolases was found in comparison with normal neural tissues but aryl-sulfatase was not increased. Cytochrome oxidase activities were markedly reduced in all tumors studied. The proportionate reduction with respect to normal brain was comparable to that noted in man. No differences were found with respect to fairly well differentiated gliomas.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00690381
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Increased protoplast yield from oat leaves and bean internodes by non-injurious mechanical perturbation (1989)
    Springer
    Protoplasma 151 (1989), S. 151-157 
    Details
    ISSN: 1615-6102
    Keywords: Bean internodes ; Oat leaves ; Rubbing ; Protoplasts ; Wounding
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary A simple new technique has been developed to greatly increase the yield of protoplasts from plant organs without injury to the plant. Mechanical perturbation (MP) by non-stressful rubbing of oat leaf segments and bean internodes yielded ten to twenty times more viable protoplasts than did controls. The increase in protoplast yield due to MP is best manifested, if the organs are excised and transferred to the cellulytic enzymes immediately after MP is given to the intact organ. The enzymes begin digesting from the lower end of the bean internodes and proceed acropetally. Vacuum infiltration of control oat leaf segments for 15 min with enzyme solution resulted in increased yield but less than due to MP. Increased levels of calcium (10 mM) in the medium decreased the yield of protoplasts from both control and MP-treated plant organs. EGTA significantly increased the yield of protoplasts from control oat leaf segments and marginally over that found in the control bean internodes. Cycloheximide increased the yield of protoplasts from oat leaf segments but not from bean internodes. It is suggested that MP may increase the susceptibility of cell wall polymers to cellulytic enzymes by reducing calcium cross linking. MP is thus a tool for increasing the yield of protoplasts from plant organs without causing injury.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01403452
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Candida amapae, a new amino acid-requiring yeast from the Amazonian fruitParahancornia amapa (1995)
    Springer
    Journal of industrial microbiology and biotechnology 14 (1995), S. 531-535 
    Details
    ISSN: 1476-5535
    Keywords: Methionine auxotrophy ; Amazonian fruit ; Candida amapae
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: Summary A new species of oligotrophie, methionine-or cysteine-requiring yeast was isolated from ripening fruits of the AmazonianParahancornia amapa tree. This species resemblesCandida sorboxylosa and species ofIssatchenkia but is nonfermentative and forms abundant pseudomycelia. The new species was isolated only fromP. amapa fruits, and not from other fruits or the drosophilid vectors of the yeast community of the deteriorating fruit. The taxon is regarded as indigenous to the amapa fruit or inoculated by vectors other thanDrosophila before ripening. A description of the new speciesCandida amapae is given.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01573969
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    Paper (German National Licenses)
    Fulltext
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