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  • Biochemistry and Biotechnology  (1)
  • Lethal effects  (1)
  • X-ray diffraction method  (1)
  • 1
    Digitale Medien
    Digitale Medien
    Springer
    Journal of thermal analysis and calorimetry 38 (1992), S. 649-655 
    ISSN: 1572-8943
    Schlagwort(e): corrosion ; high temperature oxidation ; non-isothermal kinetics ; X-ray diffraction method
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Chemie und Pharmazie
    Beschreibung / Inhaltsverzeichnis: Zusammenfassung Unter Anwendung eines Diffraktometers mit einer Hochtemperatureinheit wurde unterhalb 570°C mittels Röntgendiffraktion die Oxidation von Fe-ARMCO untersucht. Die Oxidationsprodukte wurden in situ bestimmt und das Differenzverfahren ergabY(T)-Kurven, die die globalen Änderungen zeigen. Diese Kurven wurden anhand der mikroskopisch bestimmten resultierenden Dicken der Oxidschicht kalibriert, wobei man die Funktionx(T) erhält. Dieser Kurve wurde eine berechnete, die Arrheniussche Beziehung enthaltende Funktion angepaßt, wodurch man die Aktivierungsenergie und die Oxidations Geschwindigkeitskonstante als Funktion der Temperatur erhält.
    Notizen: Abstract Temperature resolved X-ray diffraction using a diffractometer equipped with a high temperature device was applied to the study of the oxidation of Fe-ARMCO below 570°C. The oxidation products are identified in situ and the difference method yields curvesY(T) showing the global changes. These curves were calibrated to the microscopically measured end thickness of the oxide layer obtaining the growthx(T). A calculated curve including the Arrhenius relation was fitted to thex(T) curve yielding activation energy and values of the oxidation rate constant as a function of temperature.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 2
    Digitale Medien
    Digitale Medien
    Springer
    Molecular genetics and genomics 259 (1998), S. 449-456 
    ISSN: 1617-4623
    Schlagwort(e): Key words Mouse PP1 ; Schizosaccharomyces pombe ; Overexpression ; Lethal effects ; Deletion mutants
    Quelle: Springer Online Journal Archives 1860-2000
    Thema: Biologie
    Notizen: Abstract Three mouse genes encoding type 1 protein phosphatase isotypes α, γ and δ were expressed in Schizosaccharomyces pombe cells under the control of the regulatable promoter nmt1. In the repressed state, basal expression of mouse genes was able to rescue the S. pombe mutant dis2-11. An integrated mouse gene could not, however, genetically complement S. pombe double mutants carrying disrupted dis2 and sds21 protein phosphatase genes. Overexpression of any of the three mouse protein phosphatase1 isotypes produced growth arrest and loss of viability in wild-type S. pombe cells. Overexpressing cells showed defects in chromosome distribution and in the formation of septa. These defects are characteristic of the expression of the mammalian protein phosphatases in S. pombe, since they are not observed after overexpression of endogeneous S. pombe type 1 protein phosphatases. Overexpression of a truncated version of isotype δ, which lacked protein phosphatase activity, reproduced most of the characteristics of the lethal phenotype. We conclude that the lethal effect may be due to interactions with essential cell cycle proteins.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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  • 3
    Digitale Medien
    Digitale Medien
    New York, NY [u.a.] : Wiley-Blackwell
    Cell Biochemistry and Function 11 (1993), S. 211-219 
    ISSN: 0263-6484
    Schlagwort(e): Lectins ; cocaine ; morphine ; human peripheral blood mononuclear cells (PBMNC) ; common acute lymphoblastic leukemia antigen (CALLA) ; neutral endopeptidase 3.4.24.11 (NEP) ; phospholipase C; 5′-nucleotidase ; subcellular localization ; Chemistry ; Biochemistry and Biotechnology
    Quelle: Wiley InterScience Backfile Collection 1832-2000
    Thema: Biologie , Medizin
    Notizen: We have tested the effect of alkaloids (cocaine, morphine) and enkephalins on neutral endopeptidase of peripheral blood mononuclear cells activated by lectins. When treated with concanavalin A and cocaine, peripheral blood mononuclear cells showed an enhanced activity (+110 per cent) of the membrane neutral endopeptidase, which was not related to the expression of the common acute lymphoblastic leukemia antigen at the cell surface, although both molecules have the identical amino acid sequence. Phytohemagglutinin-P, morphine and synthetic enkephalins did not induce the activity of neutral endopeptidase nor the expression of common acute lymphoblastic leukemia antigen. Our findings suggested that the drugs of abuse, cocaine and morphine, affected specific membrane constituents without altering proliferation, subcellular localization of membrane enzymes or the surface immune phenotype of peripheral blood mononuclear cells.
    Zusätzliches Material: 6 Ill.
    Materialart: Digitale Medien
    Bibliothek Standort Signatur Band/Heft/Jahr Verfügbarkeit
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