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  • 1
    Electronic Resource
    Electronic Resource
    Non-specific binding of mouse myeloma IgM immunoglobulins by human myelin sheaths and astrocytes (1986)
    Springer
    Acta neuropathologica 70 (1986), S. 284-288 
    Details
    ISSN: 1432-0533
    Keywords: Myelin sheaths ; Astroglial cells ; Mouse IgM ; GFAP ; Monoclonal antibodies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The immunoreactivity of purified mouse myeloma IgM immunoglobulins (mouse IgM) to human myelin sheaths and astroglial cells was evaluated with the peroxidase-antiperoxidase method on paraffin-embedded tissues from human gliomas and areas of multiple sclerosis, and from normal human cerebrum, spinal cord and spinal nerve roots. The mouse IgM reacted positively with central and peripheral myelin sheaths and, as shown independently by others, with the cytoplasm of neoplastic and reactive astroglia. Parallel immunostaining of successive sections with an anti-glial fibrillary acidic protein (GFAP) serum and/or the anti-Leu 7 monoclonal antibody was of considerable assistance in identifying the immunoreactive elements and in distinguishing specific from non-specific immunostaining of myelin sheaths and astroglia. Pretreatment with normal human serum inhibited the non-specific binding by mouse IgM without altering GFAP and Leu 7 reactivities. The non-specific binding of mouse IgM to human myelin sheaths and astroglia can therefore be overcome, and the specificity of mouse IgM monoclonal antibodies retained, by the parallel immunostaining of successive sections with mouse IgM. If nonspecific binding by mouse IgM is found to occur, it can then be inhibited by preincubation with normal human serum without loss of specific antigenicity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00686085
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Immunohistochemical recognition of human neuroepithelial tumors by anti-Leu 7 (HNK-1) monoclonal antibody (1986)
    Springer
    Acta neuropathologica 69 (1986), S. 227-233 
    Details
    ISSN: 1432-0533
    Keywords: Leu 7 ; HNK-1 ; Monoclonal antibody ; Gliomas ; Neuroepithelial tumors
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The immunoreactivity of the anti-Leu 7 (HNK-1) monoclonal antibody, a marker for natural killer cells, was evaluated with the peroxidase-antiperoxidase (PAP) method on sections of human paraffin-embedded tissues from 135 tumors of the central nervous system and five esthesioneuroblastomas. As shown independently by others, the antibody was found to react with most types of neoplastic neuroepithelial cells. Our findings indicate that the reaction is most often localized on the cytoplasmic membranes. The immunoreactive cell membranes were generally those of well-differentiated tumor cells and of neoplastic cells found in tumors that usually were not embryonal in nature. Parallel immunostaining either of the same or of successive sections with an anti-glial fibrillary acidic protein serum was of considerable assistance in discriminating between different immunoreactive cells, e.g., between astrocytes and cells presumed to be oligodendrocytes. Despite its cross-recognition of cells of various histogenesis, the anti-Leu 7 monoclonal antibody can, in well-defined circumstances, elucidate specific differential diagnostic problems involving neurogenic neoplasms that cannot be resolved with routine staining techniques.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00688298
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Immunohistochemical recognition of human nerve sheath tumors by anti-Leu 7 (HNK-1) monoclonal antibody (1985)
    Springer
    Acta neuropathologica 68 (1985), S. 319-324 
    Details
    ISSN: 1432-0533
    Keywords: Anti-Leu 7 ; HNK-1 ; Monoclonal antibody ; Myelin-associated glycoprotein ; Schwannoma ; Neurofibroma
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Using relatively high dilutions of anti-Leu 7 monoclonal antibody and a four-step peroxidase-antiperoxidase (PAP) reaction in paraffin-embedded tissues, we tested the affinity of this antibody to the cells of 47 human nerve sheath tumors and 22 other tumors in which the differential diagnosis with nerve sheath neoplasms is known to arise. Of all the nerve sheath tumors studied 68%, including 80% of the schwannomas, contained anti-Leu 7-positive cells. All 22 non-schwannian neoplasms were entirely, negative. Specimens of eight experimental malignant rat schwannomas were also negative for anti-Leu 7 antibody. Our findings suggest that anti-Leu 7 monoclonal antibody is a promising marker that may facilitate the differential diagnosis between human Schwann cell and non-schwann cell neoplasms.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00690835
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Periventricular leukomalacia: a correlation study between real-time ultrasound and autopsy findings (1985)
    Springer
    Neuroradiology 27 (1985), S. 292-300 
    Details
    ISSN: 1432-1920
    Keywords: Brain ; ultrasound ; leukomalacia ; preterm
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The aim of this study was to validate the accuracy of real-time ultrasound (US) in the diagnosis of periventricular leukomalacia (PVL). US changes of PVL were correlated with autopsy results. During a 12-month period, all premature infants of 34 weeks' gestation or less (group A) and all neonates of more than 34 weeks' gestation who presented with abnormal neurological signs (group B) were studied with an ATL mechanical sector scanner (5 and 7.5 MHz). The overall incidence of PVL was 13.3%. In group A (n=83), 13 infants had PVL and 3 died. In group B (n=36), three developed PVL and two died. Autopsy was performed in the five infants. US revealed the sequence of lesion:-the early stage with increased echogenicity in the periventricular white matter,-the late stage with area of reduced echogenicity appearing in the most echogenic zone and resulting in cystic cavitation. Autopsy confirmed PVL lesions in all five infants. The increased echogenicity corresponded to necrosis with either vascular congestion and/or secondary bleeding, the reduced echogenicity to cystic degeneration with gliosis. US scan be used for the detection of PVL.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00339560
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    Paper (German National Licenses)
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