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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Biology and fertility of soils 7 (1989), S. 341-345 
    ISSN: 1432-0789
    Keywords: ATP content ; Bulk soil ; CO2 production ; Mineral N ; Nitrification inhibitor ; Rhizosphere ; Sewage sludge ; Hordeum vulgare
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary The microbial activity at the soil-root interface (rhizosphere) of barley was examined using a rhizobox system. In this system, the soil was placed in several compartments separated from each other by a 500-mesh nylon cloth. Plants were grown in the central compartment and after a 2-month growing period the roots were still confined to this compartment. The soil from each compartment was then analyzed for ATP, NO3 /−, total N, total C and CO2 production. The increase in ATP concentration was found in a range of 4 mm around the roots. The ATP content and CO2 production across the rhizosphere were correlated in all the soils used, but changes in NO3 − were not correlated with ATP changes. The range of NO3 − change was wider than that of the ATP change, indicating that NO3 − production is not influenced by the biological activity in the rhizosphere.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1573-5036
    Keywords: Barley root ; cDNA ; dioxygenase ; Ids2 ; iron deficiency ; mugineic acid
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract A λZAPII-cDNA library constructed from poly(A)+-RNA isolated from Fe-deficient barley roots was used for differential screening of barley roots grown in the presence and absence of Fe. Among seven clones that hybridised specifically to the probe for Fe deficiency, one clone (Ids2) was sequenced. Using a part of the cDNA sequence as a probe, a genomic-DNA library was probed and a corresponding DNA clone was isolated and sequenced. The predicted amino acid sequence resembled 2-oxoglutarate-dependent dioxygenase. Ids2 had a metal regulatory element as well as Cu regulatory elements of CUP1 gene of yeast MT.
    Type of Medium: Electronic Resource
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