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  • C7 deficiency  (1)
  • IL-1β  (1)
  • IgG-sensitized latex  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Modulation of C2 and C3 gene expression of human peripheral blood monocytes by interleukin 1β, interferon γ, tumor necrosis factor α and lipopolysaccharide (1992)
    Springer
    Cellular and molecular life sciences 48 (1992), S. 1148-1150 
    Details
    ISSN: 1420-9071
    Keywords: IL-1β ; IFNγ ; LPS ; C2 ; C3
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract The effect of interleukin 1β (IL-1β), interferon γ (IFN-γ), tumor necrosis factor α (TNFα) and lipopolysaccharide (LPS) on the expression of the C2 and C3 genes in human adherent monocytes was studied. Stimulation of monocytes with IFN-γ increased both C2 and C3 mRNA. IL-1β also increased C2 mRNA level, whereas C3 gene expression was not enhanced. TNFα failed to increase either C2 or C3 mRNA. LPS increased C2 mRNA, but suppressed C3 gene expression. These results suggest that C2 and C3 production by monocytes is regulated by IL-1β and IFN-β in the local tissues.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01948011
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    C7 deficiency and persistent haematuria (1988)
    Springer
    European journal of pediatrics 147 (1988), S. 516-517 
    Details
    ISSN: 1432-1076
    Keywords: C7 deficiency ; haematuria ; Chronic glomerulonephritis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A 14-year-old boy had persistent haematuria along with complete C7 deficiency. No significant changes in glomeruli and tubules were found in a renal biopsy specimen by light microscopy and immunofluorescence gave negative results for immune deposits. Electron microscopic examination demonstrated an attenuation of the glomerular capillary basement membrane without lamination and a diagnosis of thin basement membrane disease was made. It would be difficult to conclude that patients with C7 deficiency were predisposed to develop glomerulonephritis caused by immunologic aberrations. A family study failed to provide evidence of an association of C7 deficiency and thin basement membrane disease.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00441978
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Preparation of latex sensitized with rabbit IgG antibody for slide reversed passive agglutination (1992)
    Springer
    Veterinary research communications 16 (1992), S. 265-272 
    Details
    ISSN: 1573-7446
    Keywords: agglutination ; IgG-sensitized latex ; lactoferrin ; reversed passive latex agglutination ; RPLA
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract A method is described for preparing latex particles sensitized with IgG antibody (IgG-sensitized latex) applicable to the slide reversed passive agglutination (RPLA) test. Soap-free latex (latex) was sensitized with IgG which had been isolated from rabbit anti-bovine lactoferrin serum using protein A Sepharose CL 4B. Unadsorbed protein-binding sites on the surface of latex were blocked with bovine serum albumin (BSA). IgG-sensitized latex that gave better agglutination in RPLA could be selectively obtained by centrifugation at 19 900g for 15 min in 0.01 mol/L glycine buffer (pH 7.3; specific gravity 1.042) containing 3% NaCl, 5% saccharose and 2% choline chloride. By dispersing this IgG-sensitized latex in 0.01 mol/L glycine buffer (pH 7.3) containing 1–2% BSA, a uniformly suspended, highly reactive, readily agglutinable preparation was obtained.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01839325
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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