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  • 1
    Electronic Resource
    Electronic Resource
    Sorghum mitochondrial orf25 and a related chimeric configuration of a male-sterile cytoplasm (1996)
    Springer
    Current genetics 29 (1996), S. 265-274 
    Details
    ISSN: 1432-0983
    Keywords: Key words  Sorghum ; Mitochondrial DNA ; orf25 ; Cytoplasmic male sterility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract   We describe fundamental characteristics of sorghum mitochondrial orf25, urf209, and a related chimeric configuration, orf265/130, which is restricted to the IS1112C source of cytoplasmic male sterility in sorghum. Transcripts of urf209 are edited at ten nucleotides, resulting in nine amino-acid changes predicted from genomic sequences. The cDNA-predicted polypeptide product is 23.6 kDa, while Western blot analyses identify a product of 20 kDa. Transcription of urf209 is characterized by one or two transcripts, dependent on nuclear background, but this difference is not related to male fertility status. The orf265/130 chimeric region includes 288 bp 95% identical to sequences 5′ to maize T-cytoplasm T-urf13 and atp6, which includes a common transcription initiation site, and terminates with a recombinational event involving urf209. The urf209 similarity extends 189 bp, followed by sequences duplicated 5′ to sorghum atp6-2. Sequences immediately 3′ to the atp6-2 similarity include a second in-frame start codon, defining orf130. Structural features 5′ to orf130 are shared with motifs found 5′ to several translated mitochondrial open reading frames. The orf265/orf130 configuration is uniquely transcribed, and transcripts of orf130 exhibit one silent RNA editing event. Transcription in somatic cells is not altered by male fertility status.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002940050045
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  • 2
    Electronic Resource
    Electronic Resource
    Fertility restoration and mitochondrial nucleic acids distinguish at least five subgroups among cms-S cytoplasms of maize (Zea mays L.) (1985)
    Springer
    Theoretical and applied genetics 71 (1985), S. 5-15 
    Details
    ISSN: 1432-2242
    Keywords: Cytoplasmic male sterility ; cms-S ; Mitochondrial DNA ; Double-stranded RNA ; Gametophytic restoration of fertility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Differences in fertility restoration and mitochondrial nucleic acids permitted division of 25 accessions of S-type male sterile cytoplasm (cms-S) of maize into five subgroups: B/D, CA, LBN, ME, and S(USDA). S cytoplasm itself (USDA cytoplasm) was surprisingly not representative of cms-S, since only two other accessions, TC and I, matched its mitochondrial DNA pattern. CA was the predominant subgroup, containing 18 of the 25 accessions. The B/D and ME subgroups were the most fertile and LBN the most sterile. The exceptional sterility of LBN cytoplasm makes it the most promising of the 25 cms-S accessions for the production of hybrid seed. The most efficient means of quantifying the fertility of the subgroups was analysis of pollen morphology in plants having cms-S cytoplasm and simultaneously being heterozygous for nuclear restorer-of-fertility (Rf) genes. This method took advantage of the gametophytic nature of cms-S restoration. The inbred NY821LERf was found to contain at least two restorer genes for cms-S. Fertility differences were correlated with mitochondrial nucleic acid variation in the LBN, ME, and S (USDA) subgroups.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00278246
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  • 3
    Electronic Resource
    Electronic Resource
    Genetic analysis of nuclear control of T-urf13/orf221 transcription in T cytoplasm maize (1992)
    Springer
    Theoretical and applied genetics 84 (1992), S. 891-898 
    Details
    ISSN: 1432-2242
    Keywords: Nuclear ; mitochondrial interactions ; Cytoplasmic male sterility ; Maize ; Mitochondrial gene transcription
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The mitochondrial gene T-urf13 in T cytoplasm maize is associated with sensitivity to disease toxins and with cytoplasmic male sterility. T-urf13 is co-transcribed with an open reading frame designated orf221. We have detected alterations in the transcription of the T-urf13/orf221 region that are affected by nuclear genotype. There are multiple mRNA transcripts generated from the T-urf13/orf221 region, one of which is a processed 1538-nucleotide (nt) transcript. This 1538-nt transcript is present in Wf9 (T), but was not found in mitochondrial RNAs (mtRNAs) from maize inbreds B14A (T) and 33-16 (T). For B14A (T) a 1500-nt transcript was detected and for 33-16 (T) a 1400-nt transcript was detected. In F1 progeny of the cross of Wf9 (T) x 33-16 (N), only the 1400-nt transcript was present. Genetic analyses revealed this processing event is nuclear controlled with dominant gene action and is independent of nuclear restorer gene Rf1-associated processing events. T-urf13/orf221 transcriptional patterns were shown to vary in both sterile and fertile states. Segregation analysis of a 1100-nt orf221-specific transcript indicated that the genetic basis of nuclear control for the presence of this transcript was relatively simple. Analysis of the A188 (T4) tissue culture mutant, which has reverted to male fertility but displays the same T-urf13/orf221 transcript pattern as A188 (T), indicated no DNA sequence differences between T4-orf221 and T-orf221. Presence of the nuclear gene Rf2 was not necessary for expression of the T4 cytoplasm-associated malefertile phenotype.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00227401
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  • 4
    Electronic Resource
    Electronic Resource
    Mitochondrial DNA duplication/deletion events and polymorphism of the C group of male sterile maize cytoplasms (1987)
    Springer
    Theoretical and applied genetics 73 (1987), S. 646-653 
    Details
    ISSN: 1432-2242
    Keywords: Cytoplasmic male sterility ; Mitochondrial DNA ; Recombination
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Five accessions of members of the C group of male sterile maize cytoplasms (BB, C, ES, PR, and RB) in two nuclear backgrounds (A619 and A632) were examined to elucidate the nature of mitochondrial genome diversity within a related group of cytoplasms. Cosmid and plasmid clones carrying single copy and recombinationally active sequences from N and S cytoplasms of maize were used as probes. Although restriction patterns are quite similar, each of the five could be discriminated by evidence of sequence duplication and recombination, deletion of recombinationally active sequences of N, normal cytoplasm, population of mini-circular DNAs, and by restriction patterns. Each member of the group carried a 1,913 bp minicircular mtDNA, while all entries but RB carried a 1,445 bp minicircular mtDNA. Members of the C group clearly are not molecularly identical; evolution of the group included principal genome reorganization involving sequence duplication/deletion events, apparently independent of the cms trait.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00260771
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  • 5
    Electronic Resource
    Electronic Resource
    Large double-stranded RNA molecules in Phaseolus vulgaris L. are not associated with cytoplasmic male sterility (1988)
    Springer
    Theoretical and applied genetics 76 (1988), S. 59-63 
    Details
    ISSN: 1432-2242
    Keywords: Common bean ; Cryptic virus ; CMS
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Two large double-stranded RNA molecules, 15 and 16 kilobases, were detected in cytoplasmic male sterile (CMS) Phaseolus vulgaris by agarose gel electrophoresis. A number of smaller RNA molecules were observed in ‘Sprite’, a maintainer line, and recurrent backcrossing of CMS P. vulgarisx‘Sprite’ resulted in a combined electrophoretic pattern of the two large and numerous small RNA molecules. The large RNA molecules were seed and pollen-transmissible, but were not transmitted by grafting. The RNAs were present in revertant and restored lines derived from CMS-Sprite and therefore were not associated with the cytoplasmic male sterile trait.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00288832
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  • 6
    Electronic Resource
    Electronic Resource
    Influence of nuclear background on transcription of a maize mitochondrial region associated with Texas male sterile cytoplasm (1987)
    Springer
    Molecular genetics and genomics 210 (1987), S. 399-406 
    Details
    ISSN: 1617-4623
    Keywords: Maize ; Cytoplasmic male sterility ; Mitochondrial DNA ; Nuclear-mitochondrial interaction
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Transcripts of the maize mitochondrial genes atp6, urf13-T and ORF25 were examined by Northern analysis from five normal (N), Texas male sterile (T), and T male sterile cytoplasm lines restored to fertility. A 5 kb DNA region containing the atp6 promoter is duplicated 5′ to urf13-T and ORF25 in T cytoplasm. DNA sequence differences in the 5 kb repeat detected between N and T cytoplasms may be related to atp6 transcriptional differences between the two cytoplasms. Nuclear restorer genes directed an RNA processing event that apparently decreased the abundance of mature urf13-T transcripts, which may correlate with the observed reduction of the 13 kDa gene product in restored lines. Fertility restoration had no effect on the transcription of atp6. Nuclear background, distinct from fertility restoration, also affected the abundance of urf13-T transcripts and directed the synthesis of an ORF25-specific transcript.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00327189
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  • 7
    Electronic Resource
    Electronic Resource
    urf13-T of T cytoplasm maize mitochondria encodes a 13 kD polypeptide (1987)
    Springer
    Plant molecular biology 9 (1987), S. 121-126 
    Details
    ISSN: 1573-5028
    Keywords: cytoplasmic male sterility ; disease susceptibility ; Cochliobolus heterostrophus ; Phyllosticta maydis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Polyspecific antibody to a 17 amino acid synthetic peptide from the maize T-cytoplasm urf13-T mitochondrial open reading frame immunoprecipitated a 13 kD polypeptide from 35S-methionine incorporations of T cytoplasm maize. Male-fertile, toxin-insensitive mutants in which the urf13-T sequence is deleted do not synthesize the 13 kD polypeptide. A mutant designated T-4, which carries a 5 bp insertion and a premature stop codon, synthesizes a truncated polypeptide, corresponding to an open reading frame of 8.3 kD. Thus the 13 kD polypeptide is trunctated or absent in mutants expressing male fertility and toxin insensitivity in T-cytoplasm maize.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00015644
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  • 8
    Electronic Resource
    Electronic Resource
    Mitochondrial DNA rearrangements in Pennisetum associated with reversion from cytoplasmic male sterility to fertility (1987)
    Springer
    Plant molecular biology 9 (1987), S. 277-286 
    Details
    ISSN: 1573-5028
    Keywords: pearl millet ; mitochondria ; chloroplast ; revertant ; restriction endonuclease fragment patterns ; cytoplasmic male sterility
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Endonuclease restriction fragment patterns of Pennisetum americanum L. mitochondrial DNAs (mtDNAs) from a cytoplasmic male-sterile (CMS-A1), fertile revertants and a normal fertile cytoplasm were variable, while chloroplast DNA from those lines lacked variation. Comparisons between mtDNAs of CMS-A1 (parental) and fertile revertant lines revealed the presence of a unique 4.7 kbp PstI fragment in the sterile line that was not detected in any of the revertant lines. A 9.7 kbp PstI fragment was found in all of the revertants, but not in the CMS-A1. Neither of those fragments was found in the normal cytoplasm mtDNA. Hybridization studies revealed two sets of multiple homologies: 1) the 4.7 kbp fragment had homology with a 10.9 kbp and a 13.6 kbp fragment; and 2) the 9.7 kbp fragment was homologous with the 13.6 kbp fragment. The presence of those two repeated mitochondrial sequences on the altered fragments suggests that they may be involved in the recombinational associated events with reversion from CMS to fertility in P. americanum.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00166463
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