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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Archives of microbiology 135 (1983), S. 110-114 
    ISSN: 1432-072X
    Keywords: Emulsifying agent ; 2,4,5-Trichlorophenoxy-acetic acid ; Pseudomonas cepacia AC1100
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The culture supernatant of Pseudomonas cepacia (ATCC 39027) grown on 2,4,5-trichlorophenoxyacetic acid (2,4,5-T) was found to contain an agent which can emulsify 2,4,5-T. The emulsion is stable for several hours. The emulsifying agent was produced in response to growth on 2,4,5-T, although some emulsification was observed in culture supernatant of glucose grown cells. The emulsifying agent is most active with 2,4,5-T but has some activity towards other chlorinated compounds such as chlorophenols. In growing culture the emulsifying agent adheres to the cell surface as a slimy layer. The emulsifying agent is believed to have a role in transport of 2,4,5-T into the cells.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Molecular genetics and genomics 218 (1989), S. 266-271 
    ISSN: 1617-4623
    Keywords: Benzoate degradation ; Positive regulation ; Site-specific mutagenesis ; Primer extension ; Catechol
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Pseudomonas putida utilizes the catBC operon, which encodes cis,cis-muconate lactonizing enzyme I (MLEI; EC 5.5.1.1) and muconolactone isomerase (MI; EC 5.3.3.4), for growth on benzoate as a sole carbon source. This operon is positively regulated, and the promoter is located 64 bp upstream of the catB translational start site. Using site-specific mutagenesis, we identified nucleotides that influenced the induction of this promoter. Promoter activity was monitored with the promoter probe vector pKT240. Transcription of mRNA from mutant promoters was determined by primer extension mapping. Comparison of the initiation start site of mutant promoters with that of the wild-type promoter identified a single functional promoter.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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