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  • Cell chain formation  (1)
  • Cell wall structure  (1)
  • Nicotiana  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Ultrastructure of cell wall and plugs of tobacco pollen tubes after chemical extraction of polysaccharides (1982)
    Springer
    Planta 154 (1982), S. 241-250 
    Details
    ISSN: 1432-2048
    Keywords: Callose ; Cellulose ; Nicotiana ; Pectin ; Pollen tube wall
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Tobacco pollen tubes grown in vitro and from pollinated tobacco styles were treated by chemical solvents to remove one or more of the following polysaccharides from the tube walls: pectin (ethylenediamine tetraacetic acid); hemicellulose (alkali); callose (alkali; potassium hypochlorite); cellulose (cuprammonium); and all polysaccharides with exception of cellulose (H2O2/glacial acetic acid). Both the inner tube wall, which we had regarded as the secondary wall, and the plugs contained, in addition to callose, microfibrils of cellulose and “non-cellulosic” microfibrils that had “pectin-like” properties. When using the expressions callosic or callose layer and callose plugs in reference to pollen tubes, one should realize that they do not imply the exclusive presence of callose in the inner tube wall layer and its localized thickenings.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00387870
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Development of subprotoplasts from in vitro-grown tobacco pollen tubes (1988)
    Springer
    Sexual plant reproduction 1 (1988), S. 103-113 
    Details
    ISSN: 1432-2145
    Keywords: Cell chain formation ; Cell wall regeneration ; Cytoplast ; Karyoplast ; Nicotiana (pollen tube subprotoplast) ; Tube formation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Plasmolyzed pollen tubes of Nicotiana tabacum each released one to three subprotoplasts from their tips when treated with wall-degrading enzymes. Wall regeneration and the further development of the subprotoplasts were studied by both light and electron microscopy. Karyoplasts and cytoplasts incubated in a poor culture medium regenerated a cell wall within 60 min; cellulose microfibrils and callose were shown to be present. In 30%–40% of the cells, one-third of which were nucleate, cell chains and tubes developed by polar growth in a ratio of about 1∶1. They sometimes reached a length 7–9 times the diameter of the former subprotoplast within 5 h of incubation. With longer incubation periods the cell wall became two-layered, its ultrastructure resembling that of the pollen tubes. The capacity of cytoplasts to regenerate a wall and develop cell chains and tubes can be explained by the properties ascribed to the cytoplasm of pollen tubes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00189269
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Cell wall formation in zoospores of Allomyces arbuscula (1976)
    Springer
    Archives of microbiology 109 (1976), S. 37-43 
    Details
    ISSN: 1432-072X
    Keywords: Allomyces ; Development of meiospores ; Cell wall structure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Development of haploid meiospores of Allomyces arbuscula into germling cells with rhizoids and hyphae was followed during incubation in complete growth medium. The surface structure of encysted meiospores, rhizoids and hyphae before and after extraction of amorphous materials with ethanolic KOH was studied by means of carbon-platinum replicas. After 2–3 min incubation in complete medium 10% of the meiospores were surrounded by a cell wall containing microfibrils embedded in a matrix. Structure of cell walls of encysted meiospores, rhizoids, and hyphae differ from one another by the location of amorphous materials and by the arrangement of chitin microfibrils.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00425110
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    Paper (German National Licenses)
    Fulltext
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