ISSN:
1432-2145
Keywords:
Cell chain formation
;
Cell wall regeneration
;
Cytoplast
;
Karyoplast
;
Nicotiana (pollen tube subprotoplast)
;
Tube formation
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Summary Plasmolyzed pollen tubes of Nicotiana tabacum each released one to three subprotoplasts from their tips when treated with wall-degrading enzymes. Wall regeneration and the further development of the subprotoplasts were studied by both light and electron microscopy. Karyoplasts and cytoplasts incubated in a poor culture medium regenerated a cell wall within 60 min; cellulose microfibrils and callose were shown to be present. In 30%–40% of the cells, one-third of which were nucleate, cell chains and tubes developed by polar growth in a ratio of about 1∶1. They sometimes reached a length 7–9 times the diameter of the former subprotoplast within 5 h of incubation. With longer incubation periods the cell wall became two-layered, its ultrastructure resembling that of the pollen tubes. The capacity of cytoplasts to regenerate a wall and develop cell chains and tubes can be explained by the properties ascribed to the cytoplasm of pollen tubes.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00189269
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