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  • Tracheal lavage  (4)
  • Chemical Engineering  (3)
  • Somatic embryogenesis  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Agrobacterium-mediated genetic transformation of California poppy, Eschscholzia californica Cham., via somatic embryogenesis (2000)
    Springer
    Plant cell reports 19 (2000), S. 1006-1012 
    Details
    ISSN: 1432-203X
    Keywords: Key words Agrobacterium tumefaciens ; Benzylisoquinoline alkaloids ; California poppy ; Eschscholzia californica Cham. ; Genetic transformation ; Somatic embryogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  An efficient Agrobacterium-mediated protocol for the stable genetic transformation of Eschscholzia californica Cham. (California poppy) via somatic embryogenesis is reported. Excised cotyledons were co-cultivated with A. tumefaciens strain GV3101 carrying the pBI121 binary vector. Except for the co-cultivation medium, all formulations included 50 mg l−1 paromomycin as the selective agent and 200 mg l−1 timentin to eliminate the Agrobacterium. Four to five weeks after infection, paromomycin-resistant calli grew on 80% of explants in the presence of 2.0 mg l−1 1-naphthaleneacetic acid (NAA) and 0.1 mg l−1 6-benzylaminopurine (BAP). Calli were cultured on somatic embryogenesis induction medium containing 1.0 mg l−1 NAA and 0.5 mg l−1 BAP, and somatic embryos were visible on 30% of the paromomycin-resistant calli within 3–4 weeks. Three to four weeks after the somatic embryos were transferred to phytohormone-free plant regeneration medium, 32% converted to paromomycin-resistant plants. Detection of the neomycin phosphotransferase gene and high levels of β-glucuronidase (GUS) mRNA and enzyme activity, and the cytohistochemical localization of GUS activity in all plant tissues confirmed the integrative transformation of the regenerated plants. The normal alkaloid profile of California poppy was unaffected by the transformation process; thus, the reported protocol could serve as a valuable tool to investigate the molecular and metabolic regulation of the benzophenanthridine alkaloid pathway.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990000213
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  • 2
    Electronic Resource
    Electronic Resource
    High-efficiency somatic embryogenesis and plant regeneration in California poppy, Eschscholzia californica Cham. (2000)
    Springer
    Plant cell reports 19 (2000), S. 421-426 
    Details
    ISSN: 1432-203X
    Keywords: Key words California poppy ; Eschscholzia californica ; Plant regeneration ; Somatic embryogenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The development of a rapid protocol for high-efficiency somatic embryogenesis and plant regeneration from seed-derived embryogenic callus cultures of California poppy (Eschscholzia californica Cham.) is reported. The optimized procedure required less than 13 weeks from the initiation of seed cultures to the recovery of plantlets and involved the sequential transfer of cultures onto solid Murashige and Skoog basal medium containing three different combinations of growth regulators. All steps were performed at 25  °C. Friable primary callus was induced from seeds of E. californica cultured on medium supplemented with 1.0 mg l−1 2,4-dichlorophenoxyacetic acid. The primary callus was transferred to medium containing 1.0 mg l−1 1-naphthaleneacetic acid and 0.5 mg l−1 6-benzylaminopurine to establish embryogenic callus and promote somatic embryogenesis. Regenerated plantlets were recovered after the conversion of somatic embryos on medium containing 0.05 mg l−1 6-benzylaminopurine and showed normal development. Embryogenic callus was induced at a frequency of 85%, an average of 45 somatic embryos were produced per callus, 90% of the somatic embryos converted, and about 70% of the plantlets were recovered in soil. The growth rate of somatic embryo-derived shoots could be increased by gibberellic acid treatment, but the resulting plantlets were hyperhydritic.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s002990050750
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  • 3
    Electronic Resource
    Electronic Resource
    Histamine release into cracheal lumen and bronchial reactivity (1983)
    Springer
    Research in experimental medicine 182 (1983), S. 153-166 
    Details
    ISSN: 1433-8580
    Keywords: Tracheal lavage ; Allergen ; Acetylcholine, histamine ; Hyperreactivity
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The influence of tracheal lavage with an allergen on the airway response to bronchial challenge with histamine, acetylcholine, and ascaris extract was investigated in 36 dogs. High histamine concentrations were measured in the tracheal solution after lavage with allergen. This amine, released on the mucosal surface, was observed in only very small concentrations in the circulation. Airway response to acetylcholine inhalation was significantly increased after tracheal lavage with allergen. This hyperreactivity of the lower airways induced by tracheal lavage with an allergen suggests that a reflex mechanism is involved. After histamine inhalation a significantly larger antigen-induced histamine release to tracheal lumen was observed. No influence on airway response was found after tracheal lavage with H2O.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01851121
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  • 4
    Electronic Resource
    Electronic Resource
    Histamine release into cracheal lumen and bronchial reactivity (1983)
    Springer
    Research in experimental medicine 182 (1983), S. 167-175 
    Details
    ISSN: 1433-8580
    Keywords: Tracheal lavage ; Pronase ; Acetylcholine ; Histamine
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The influence of tracheal lavage in a restricted area, with a proteolytic enzyme (Pronase) on the bronchial response to challenge with ACH and AE was studied in 15 dogs. Histamine concentrations measured in the tracheal liquid after lavage with Pronase were smaller than the values after tracheal lavage with allergen. Airway response to ACH inhalation was significantly increased after tracheal lavage with Pronase. Effects of proteolytic enzymes and allergens on the tracheal wall are compared. Allergens and proteolytic enzymes evidence a different mode of action in increasing reactivity of peripheral airways.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01851122
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  • 5
    Electronic Resource
    Electronic Resource
    Histamine release into tracheal lumen and bronchial reactivity (1983)
    Springer
    Research in experimental medicine 183 (1983), S. 55-65 
    Details
    ISSN: 1433-8580
    Keywords: Tracheal lavage ; Compound 48/80 ; Acetylcholine ; Histamine release
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The influence of tracheal lavage with compound 48/80 on the bronchial response to challenge with ACH and AE was studied in 24 animals. Furthermore, the effect of i.v. administration of 48/80 was compared to its administration into the tracheal lumen. No increased histamine liberation was detected in the tracheal fluid after lavage with 48/80, nor was an influence on the bronchial response observed. These observations were the same as those described for tracheal lavage with water. The same amounts of this secretagogue induced, after i.v. administration, a high histamine release which correlates significantly with blood pressure decrease.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01851763
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  • 6
    Electronic Resource
    Electronic Resource
    Histamine release into tracheal lumen and bronchial reactivity (1983)
    Springer
    Research in experimental medicine 183 (1983), S. 67-75 
    Details
    ISSN: 1433-8580
    Keywords: Histamine ; Bronchospastic response ; Acetylcholine ; Tracheal lavage
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary The influence of tracheal lavage with histamine, and of i.v. administration of histamine on bronchospastic response was studied in 16 dogs. Airway response to acetylcholine inhalation was significantly increased after tracheal lavage with high concentrated histamine solution (1%). No influence on airway response was found after (1) tracheal lavage with lower concentrations of histamine and (2) its i.v. administration. The clinical importance of these manifestations is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01851764
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  • 7
    Electronic Resource
    Electronic Resource
    Rheological study on poly-D(-)(3-hydroxybutyrate) and its blends with poly(ethylene oxide) (1995)
    Stamford, Conn. [u.a.] : Wiley-Blackwell
    Polymer Engineering and Science 35 (1995), S. 1636-1642 
    Details
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Aspects of thermal, morphological, and rheological properties of biodegradable poly-D(-)(3-hydeoxybutyrate) (PHB) blended with poly(ethylene oxide) (PEO) have been studied. Thermal properties and morphology of the blends were examined by scanning electron microscopy and differential scanning calorimetry, respectively. A rotational theometer with parallel plate geometry was also adopted to investigate the rheological properties of these blends. In addition, dynamic ciscoelasticity was measured by a Rheovibron as functions of time and temperature. From these measurements, PHB and PEO were observed to be miscible in the melt state. In the case of the blend systen 80/20 PHB/PEO by weight, the vacant domains of the PHB were filled with PEO particles, and this morphological state enhanced the rheological properties. Furthermore, PHB and its blends were found to have high crystallinities, but to have unstable thermal behavior about Tm.
    Additional Material: 13 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pen.760352010
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    Paper (German National Licenses)
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  • 8
    Electronic Resource
    Electronic Resource
    Error in the propagation of error formula (1980)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 26 (1980), S. 168-170 
    Details
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aic.690260132
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  • 9
    Electronic Resource
    Electronic Resource
    Letters to the editor (1981)
    Hoboken, NJ : Wiley-Blackwell
    AIChE Journal 27 (1981), S. 527-528 
    Details
    ISSN: 0001-1541
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/aic.690270332
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    Paper (German National Licenses)
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