Library

X
feed icon rss

Your email was sent successfully. Check your inbox.

An error occurred while sending the email. Please try again.

Proceed reservation?

Export
  • 1
    Electronic Resource
    Electronic Resource
    Identification of the DNA damage-responsive elements of the rhp51 + gene, a recA and RAD51 homolog from the fission yeast Schizosaccharomyces pombe (1996)
    Springer
    Molecular genetics and genomics 251 (1996), S. 167-175 
    Details
    ISSN: 1617-4623
    Keywords: Key wordsSchizosaccharomyces pombe ; DNA-damage inducibility ; Damage-responsive element ; Upstream activating sequence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The Schizosaccharomyces pombe rhp51 + gene encodes a recombinational repair protein that shares significant sequence identities with the bacterial RecA and the Saccharomyces cerevisiae 1RAD51 protein. Levels of rhp51 + mRNA increase following several types of DNA damage or inhibition of DNA synthesis. An rhp51:: ura4 fusion gene was used to identify the cis-acting promoter elements involved in regulating rhp51 + expression in response to DNA damage. Two elements, designated DRE1 and DRE2 (for damage-responsive element), match a decamer consensus URS (upstream repressing sequence) found in the promoters of many other DNA repair and metabolism genes from S. cerevisiae. However, our results show that DRE1 and DRE2 each function as a UAS (upstream activating sequence) rather than a URS and are also required for DNA-damage inducibility of the gene. A 20-bp fragment located downstream of both DRE1 and DRE2 is responsible for URS function. The DRE1 and DRE2 elements cross-competed for binding to two proteins of 45 and 59 kDa. DNase I footprint analysis suggests that DRE1 and DRE2 bind to the same DNA-binding proteins. These results suggest that the DRE-binding proteins may play an important role in the DNA-damage inducibility of rhp51 + expression.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02172915
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Ethyl-methane sulphonate (EMS) induced nodulation mutants of common bean (Phaseolus vulgaris L.) lacking effective nodules (1992)
    Springer
    Plant and soil 139 (1992), S. 295-298 
    Details
    ISSN: 1573-5036
    Keywords: dry bean ; N2 fixation ; nodulation mutants ; Rhizobium leguminosarum bv. phaseoli
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Seed of white bean (Phaseolus vulgaris L. cv. OAC Rico) was treated with 0.04 M ethyl-methane sulphonate. Screening of the M2 progeny from 175 M1 lines in the presence of 1 mM nitrate revealed two nodulation mutants. One line was essentially non-nodulating in several tests, but small white nodules were observed occasionally in other tests with 16 separate Rhizobium strains. The other line formed tiny, creamy white, non-functional (ineffective) and tumor-like pseudo-nodules. The M3 and M4 progenies were fertile and bred true.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00009321
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Identification and characterization of common bean (Phaseolus vulgaris L.) lines well nodulated in the presence of high nitrate (1989)
    Springer
    Plant and soil 119 (1989), S. 237-244 
    Details
    ISSN: 1573-5036
    Keywords: field bean ; nodulation ; N2 fixation ; nitrate ; Phaseolus spp. ; Rhizobium phaseoli
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract Common bean,Phaseolus vulgaris L., is known to be ‘inefficient’ in nodulation and N2 fixation although it responds to applied nitrogen. An experiment was conducted to identify and to characterize bean cultivars nodulating in the presence of a high level of nitrogen. Sixteen cultivars and a check for inefficient nodulation, OAC Seaforth, were inoculated and grown for 40 days in replicated pots supplied with zero, 3.5 and 10.5 mM combined nitrogen as NO 3 − and NH 4 + . Seven traits relating to nodulation and N2 fixation were all significantly affected by N level (N), cultivar (Cv) and N × Cv interactions (except for root dry weight), indicating that cultivars responded differently to the N treatments. Total dry weight (W) and shoot to root ratio (S/R) increased with the increased N levels. Nodule dry weight (Wn), visual nodulation score (Nv) and nodulation index (Nx) decreased as the N increased. Percent N and N content per plant increased with the increased N level. Plant weight (W) was positively correlated with Wn, Nv and N content and negatively correlated with %N. Nodulation score was positively associated with Wn and plant N content. Genotypes superior in nodulation and N2 fixation in the presence of N were identified. Cultivars Italian Barlotti, California Light Red Kidney, Kentucky Wonder A and Pueblo 152 were selected for further testing and use in improving the nitrate tolerant nodulating characteristic of bean.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02370414
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 4
    Electronic Resource
    Electronic Resource
    Identification of the DNA damage-responsive elements of therhp51 + gene, arecA andRAD51 homolog from the fission yeastSchizosaccharomyces pombe (1996)
    Springer
    Molecular genetics and genomics 251 (1996), S. 167-175 
    Details
    ISSN: 1617-4623
    Keywords: Schizosaccharomyces pombe ; DNA-damage inducibility ; Damage-responsive element ; Upstream activating sequence
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract TheSchizosaccharomyces pombe rhp51 + gene encodes a recombinational repair protein that shares significant sequence identities with the bacterial RecA and theSaccharomyces cerevisiae RAD51 protein. Levels ofrhp51 + mRNA increase following several types of DNA damage or inhibition of DNA synthesis. Anrhp51::ura4 fusion gene was used to identify the cis-acting promoter elements involved in regulatingrhp51 + expression in response to DNA damage. Two elements, designated DRE1 and DRE2 (fordamage-responsiveelement), match a decamer consensus URS (upstream repressing sequence) found in the promoters of many other DNA repair and metabolism genes fromS. cerevisiae. However, our results show that DRE1 and DRE2 each function as a UAS (upstream activating sequence) rather than a URS and are also required for DNA-damage inducibility of the gene. A 20-bp fragment located downstream of both DRE1 and DRE2 is responsible for URS function. The DRE1 and DRE2 elements cross-competed for binding to two proteins of 45 and 59 kDa. DNase I footprint analysis suggests that DRE1 and DRE2 bind to the same DNA-binding proteins. These results suggest that the DRE-binding proteins may play an important role in the DNA-damage inducibility ofrhp51 + expression.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02172915
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    Einfluß der Korngröße auf mechanische Eigenschaften und den ungeschmierten reversierenden Gleitverschleiß von Al2O3-Keramik (1992)
    Weinheim : Wiley-Blackwell
    Materialwissenschaft und Werkstofftechnik 23 (1992), S. 329-338 
    Details
    ISSN: 0933-5137
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics
    Description / Table of Contents: Influence of grain size on mechanical properties and dry oscillating sliding wear of Al2O3-ceramicsSpecimens with average grain sizes varying between about 0.8 μm and 12 μm were produced by cold isostatic pressing of high purity Al2O3-powder followed by sintering between 1300°C and 1700°C. Hardness, Young's modulus, bending strength and fracture toughness were measured as a function of average grain sizes. Tribological tests were carried out on the different microstructures at normal laboratory air and room temperature by using a ring-on-block tribometer.Experimental results showed the dependence of mechanical properties on grain size, hardness and bending strength obeying a Hall-Petch type relation, approximately. Coefficient of friction was relatively independent of grain size under the test conditions used. However, wear intensity increased substantially if a critical grain size was surpassed. This was due to a change in mechanisms of material removal which was confirmed by scanning electron microscopical studies of the worn surfaces.
    Notes: Aus hochreinem Al2O3-Pulver wurden durch kaltisostatisches Pressen und Variation der Sintertemperatur zwischen 1300°C und 1700°C Formkörper mit unterschiedlichen mittleren Korngrößen zwischen etwa 0,8 und 12 μm hergestellt. Untersucht wurde der Einfluß der Korngröße auf mechanische und tribologische Eigen-schaften.Die Ergebnisse zeigten, daß mechanische Eigenschaften wie Härte, E-Modul, Biegebruchfestigkeit und Bruchzähigkeit von der Korngröße bzw. den Sintertemperaturen abhingen, wobei im Fall der Härte und Bruchfestigkeit näiherungsweise eine Hall-Peteh-Beziehung vorlag. Während die Reibungszahl relativ unabhängig von der Korngröße war, trat in der Verschleißintensität aufgrund geänderter Mechanismen ein starker Anstieg mit zunchmender Korngröße auf. Rasterelektronenmikroskopische Untersuchungen zeigten, daß der Verschleiß durch plastische Verformung, Mikrobruch, Kornfragmentierung und Deckschichten beeinflußt wurde.
    Additional Material: 14 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/mawe.19920230909
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 6
    Electronic Resource
    Electronic Resource
    Application of extracorporeal shock wave lithotripter (ECSWL) in orthopaedics. III. In Vitro and In Vivo studiesPart of this work was presented to the Society for Biomaterials and Orthopedic Research Society annual meetings. (1991)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Biomaterials 2 (1991), S. 171-181 
    Details
    ISSN: 1045-4861
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: The present study was conducted to compare the push-out strength of the treated and control samples obtained after implantation of intramedullary rod in canine femurs with bone cement to simulate the femoral stem implantation for 2 weeks, 3 and 6 months. Contralateral femur served as control. The result showed a significant decrease in push-out interfacial strength after shock wave treatment (average 48.4% decrease compared with control, p 〈 0.0001) which is similar to the in vitro results. There was no significant difference if the shock treatment was applied and left for 2 weeks or 4 weeks compared to the ones tested immediately after sacrifice. There were some soft tissue damage immediately following shock treatment in the focal area but this returned to normal in 2 weeks. Human cadaveric femoral bones and the canine bone (the dosage level was higher for the human bone than canine). The number of impacts used to extract the bone cement plug out of a human femur segment (5-cm long) decreased about 68% at 23 and 25 kV treatment power level. These preliminary studies indicate that the shock wave can be utilized to reduce the interfacial strength of the bone and bone cement although more studies are needed to assess its efficacy in terms of cost, long-term effect on patients and the exact mechanism of the loosening before this technique can be used clinically.
    Additional Material: 18 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jab.770020305
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 7
    Electronic Resource
    Electronic Resource
    Application of extracorporeal shock wave lithotripter (ECSWL) in orthopaedics. II. Dose-response and pressure distribution measurements (1991)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Biomaterials 2 (1991), S. 161-170 
    Details
    ISSN: 1045-4861
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: In order to apply the extracorporeal shock wave lithotripter (ECWSL) technique to the loosening of the bone-cement interface for the extraction of the cement during revision arthroplasty it is essential to know the dose-response characteristics. The present study shows that the number of shocks needed to break the interface between a 2- and 6-mm-thick bovine femoral bone and bone cement is similar to the fatigue behavior of a material, that is, Log(N) = C(kV) + D, where N is the number of shock impulses, kV is the power setting of the lithotripter machine in kilovolts, and C and D are constants. Iso-pressure distribution of the traveling shock wave front through a simulated bone in a Plexiglass® tube using Fuji® pressure film showed quantitative pressure contours from which one can understand the effective area of shock wave and its distribution. The most effective area of the shock wave was about 1.5 cm in diameter at 23 and 25 kV with pressure at least 7.0 MPa which is more than sufficient to break the bone-cement interface in tension.
    Additional Material: 16 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jab.770020304
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 8
    Electronic Resource
    Electronic Resource
    Application of extracorporeal shock wave lithotripter (ECSWL) in orthopedics. I. Foundations and overviewParts of this paper were presented at the Society for Biomaterials and Orthopedic Research Society Annual meetings. (1991)
    New York, NY [u.a.] : Wiley-Blackwell
    Journal of Applied Biomaterials 2 (1991), S. 115-126 
    Details
    ISSN: 1045-4861
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Medicine , Technology
    Notes: In this paper we have reviewed the feasibility of using a shock wave lithotripter to loosen the interface between the bone and acrylic bone cement for revision arthroplasty. We have reviewed the physics of shock wave and its applications in medicine, especially its interaction with tissues. The calculations show that the energy is greatly reduced at the interface both in the soft tissue-bone interface and cement-bone interface. On the other hand, a tensile and compressive pressure can be operated at the cement-bone interface that can cause the interface to break if the pressure exceeds the tensile strength of the cement-bone interface.Subsequent papers will deal with in vitro and in vivo application of the shock wave in the treatment of the cement-bone interface in order to weaken it and consequently for easier extraction of bone cement from the intramedullary canal.
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/jab.770020207
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 9
    Electronic Resource
    Electronic Resource
    Rheological study on poly-D(-)(3-hydroxybutyrate) and its blends with poly(ethylene oxide) (1995)
    Stamford, Conn. [u.a.] : Wiley-Blackwell
    Polymer Engineering and Science 35 (1995), S. 1636-1642 
    Details
    ISSN: 0032-3888
    Keywords: Chemistry ; Chemical Engineering
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Chemistry and Pharmacology , Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics , Physics
    Notes: Aspects of thermal, morphological, and rheological properties of biodegradable poly-D(-)(3-hydeoxybutyrate) (PHB) blended with poly(ethylene oxide) (PEO) have been studied. Thermal properties and morphology of the blends were examined by scanning electron microscopy and differential scanning calorimetry, respectively. A rotational theometer with parallel plate geometry was also adopted to investigate the rheological properties of these blends. In addition, dynamic ciscoelasticity was measured by a Rheovibron as functions of time and temperature. From these measurements, PHB and PEO were observed to be miscible in the melt state. In the case of the blend systen 80/20 PHB/PEO by weight, the vacant domains of the PHB were filled with PEO particles, and this morphological state enhanced the rheological properties. Furthermore, PHB and its blends were found to have high crystallinities, but to have unstable thermal behavior about Tm.
    Additional Material: 13 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/pen.760352010
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 10
    Electronic Resource
    Electronic Resource
    Production of penicillin in a fluidized-bed bioreactor using a carrier-supported mycelial growth (1986)
    New York, NY [u.a.] : Wiley-Blackwell
    Biotechnology and Bioengineering 28 (1986), S. 1838-1844 
    Details
    ISSN: 0006-3592
    Keywords: Chemistry ; Biochemistry and Biotechnology
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology , Process Engineering, Biotechnology, Nutrition Technology
    Notes: A carrier-supported mycelial growth of Penicillium chrysogenum was applied to penicillin fermentation system using celite as a support material. Hyphal growth through the pore matrices of the material showed strong anchorages and provided highly stable biofilm growth. With bioparticles developed in such a manner, both cell growth and penicillin production were observed to increase significantly compared to the conventional dispersed filamentous cultures. Maximum values of specific penicillin production rate were found to be constant regardless of the growth form. A three-phase fluidized-bed fermentor was designed and tested for penicillin production using the bioparticles. Two modes of operation, semicontinuous and repeated fed batch, of the fermentor were tried. It was noted that the overgrowth of free mycelia and the development of fluffy loose bioparticles caused poor mixing and made the fermentor operation quite difficult. Control of the bioparticle size and the extension of production phase were therefore considered important to maintain the reactor productivity at a desired level. From the results of repeated fed-batch operation it was found that the control of bioparticle size could be successfully achieved by phosphate-limiting culture condition. Penicillin production under this condition was also observed to be maintained at a high level (about 80% of the maximum) for at least 1 month.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/bit.260281211
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
Close ⊗
This website uses cookies and the analysis tool Matomo. More information can be found here...