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  • 1
    ISSN: 1432-203X
    Keywords: Key wordsOryza sativa ; Luciferase gene ; Transient expression ; luc ; Luminograph ; Luminometer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Transformed rice plants of var `TN1' were regenerated from immature embryos following particle bombardment with a construct containing the firefly luciferase gene as a reporter gene and the hygromycin resistance gene as a selectable marker. Expression of the luciferase gene in the presence of the substrate luciferin was visualised in the calli derived from bombarded immature embryos and in the leaves and roots of the regenerated transformed plants using a low light imaging system (luminograph). Embryogenic callus proliferation and plant regeneration were unaffected by luciferin treatment and luminograph screening. The quantitative Luc assay using samples of leaf tissue from the segregating generations gave early information about the homozygous and hemizygous state of the luc transgene.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Chichester [u.a.] : Wiley-Blackwell
    Surface and Interface Analysis 23 (1995), S. 61-68 
    ISSN: 0142-2421
    Keywords: Chemistry ; Polymer and Materials Science
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Physics
    Notes: Focused ion beam (FIB) systems using gallium liquid metal ion sources can remove material with a lateral resolution below 50 nm and can produce metal deposition at a similar resolution with ion beam-enhanced chemical vapour deposition. These capabilities have resulted in many valuable applications for the microelectronics industry. Circuit modifications are possible because existing connections can be severed and reconnected to different locations. Testing of circuitry can be enhanced by isolation of specific circuits, removal of overlayers and by creation of probe pads where desired. Grain sizes can be determined from secondary electron images by the delineation of individual grains due to orientation-dependent channeling of the ion beam. Secondary ion mass spectrometry analyses of small areas can provide ion images, elemental identification of small areas and endpoint detection with depth profiles. Scanning electron microscopy and transmission electron microscopy sections are prepared routinely using the FIB. These FIB-prepared sections are notable because specific features, such as defects, can be exposed and a range of materials including silicon, indium phosphide, gallium arsenide and even metal layers can be cut without distortion. Transmission electron micrographs of superior quality have been obtained with a large area of very uniform thickness that permits identification of features such as areas under stress.
    Additional Material: 7 Ill.
    Type of Medium: Electronic Resource
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