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  • 1
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: In an attempt to generate more variation of the type represented by the rugosus loci, r and rb, putative seed development mutants have been isolated from peas (Pisum sativum L.) following chemical mutagenesis of a round-seeded (RRRbRb) genotype. The populations segregating for wrinkled seeds at the M3 generation were examined for their starch, lipid and protein content. The starch content of the wrinkled-type variants was between 0 and 55 % with an amylose content between 0 and 80 %, compared with 51 % and 30 %, respectively, for the round-seeded parent. When the range of compositions of the wrinkled-type seed was grouped, the values indicated that similar phenotypic groups to the wild type (RRRbRb) and the rugosus types (rrRbRb, RRrbrb and rrrbrb) existed in the population. The lipid content of the putative mutants supported this conclusion. Furthermore, some of the values for starch content and composition, and for lipid content indicated that new mutants had been induced. The material represents the first report on chemically-induced mutants of legumes with altered storage product composition.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    [s.l.] : Nature Publishing Group
    Nature 82 (1910), S. 366-366 
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] ATTENTION has been directed several times in NATURE to the interest attaching to the knowledge of the rate at which the gas inside rubber balloons takes up the temperature of the air outside. In this connection the results of two registering-balloon ascents from Manchester, made at the ...
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-203X
    Keywords: Key wordsOryza sativa ; Luciferase gene ; Transient expression ; luc ; Luminograph ; Luminometer
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Transformed rice plants of var `TN1' were regenerated from immature embryos following particle bombardment with a construct containing the firefly luciferase gene as a reporter gene and the hygromycin resistance gene as a selectable marker. Expression of the luciferase gene in the presence of the substrate luciferin was visualised in the calli derived from bombarded immature embryos and in the leaves and roots of the regenerated transformed plants using a low light imaging system (luminograph). Embryogenic callus proliferation and plant regeneration were unaffected by luciferin treatment and luminograph screening. The quantitative Luc assay using samples of leaf tissue from the segregating generations gave early information about the homozygous and hemizygous state of the luc transgene.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1573-5060
    Keywords: Hordeum vulgare ; isolated microspores ; particle bombardment ; transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A highly regenerable, isolated microspore system for barley, Hordeum vulgare L. cv. Igri, has been developed which is amenable to transformation studies using particle bombardment. The system allows DNA to be delivered to microspores at the single cell stage and both transient and stable transformation events have been demonstrated. The potential advantages of using isolated microspores as the target tissue in routine transformation systems are discussed.
    Type of Medium: Electronic Resource
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