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  • 1
    Electronic Resource
    Electronic Resource
    A simple system for pea transformation (1997)
    Springer
    Plant cell reports 16 (1997), S. 513-519 
    Details
    ISSN: 1432-203X
    Keywords: Key wordsAgrobacterium tumefaciens ; Pea transformation ; Cotyledonary meristem ; Bar gene ; Phosphinothricin resistance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The lateral cotyledonary meristems of germinating Pisum sativum cv. Puget seeds were used to develop a reproducible Agrobacterium tumefaciens-mediated trans-formation system. This procedure exhibits distinct advantages over those previously reported, in that it uses dry seed as starting material, and the highly regenerable cotyledonary meristems rapidly produce transgenic shoots without an intermediate callus phase. This transformation regime facilitates the rapid generation of phenotypically normal, self-fertile plants containing functional transgenes inherited in a Mendelian fashion.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01142315
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Transformation studies in Hordeum vulgare using a highly regenerable microspore system (1955)
    Springer
    Euphytica 85 (1955), S. 113-118 
    Details
    ISSN: 1573-5060
    Keywords: Hordeum vulgare ; isolated microspores ; particle bombardment ; transformation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary A highly regenerable, isolated microspore system for barley, Hordeum vulgare L. cv. Igri, has been developed which is amenable to transformation studies using particle bombardment. The system allows DNA to be delivered to microspores at the single cell stage and both transient and stable transformation events have been demonstrated. The potential advantages of using isolated microspores as the target tissue in routine transformation systems are discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00023938
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    Computer analysis identifies sequence homologies between potential gene products of Maize Streak Virus and those of Cassava Latent Virus and Tomato Golden Mosaic Virus (1985)
    Springer
    Plant molecular biology 5 (1985), S. 125-131 
    Details
    ISSN: 1573-5028
    Keywords: DIAGON ; geminivirus ; protein sequence homology ; coat protein
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The amino acid sequences of the putative polypeptides of maize streak virus (MSV) have been systematically compared with those of cassava latent virus (CLV) and tomato golden mosaic virus (TGMV) using the programme DIAGON (8). Conserved sequences have been detected between peptides encoded by the complementary (-) sense of MSV and those of CLV and TGMV, viz; the 40 200 Mr polypeptide of CLV-1 (3) and the 40 285 Mr polypeptide of TGMV-A (4) show extensive homologies with the 17 768 Mr and 31 388 Mr polypeptides of MSV (6). Distant and variable homologies have been detected between the putative coat protein of MSV when compared with those of CLV and TGMV. No other relationships between the potential gene products of MSV and those of CLV and TGMV have been detected. The extensive homologies detected between the complementary sense encoded peptides suggest that they are derived from functional genes, and that the directly conserved sequences may contain amino acids essential to the function of these proteins. The less extensive homologies among the putative coat proteins are considered in relation to their possible structures and functions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00020095
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    Paper (German National Licenses)
    Fulltext
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