ISSN:
1432-072X
Keywords:
Clostridium rectum
;
Lindane degradation
;
Stickland reaction
;
Monochlorobenzene
;
Tetrachlorocyclohexene
;
Reductive dechlorination
Source:
Springer Online Journal Archives 1860-2000
Topics:
Biology
Notes:
Abstract For lindane degradation, a cell suspension of Clostridium rectum strain S-17 demands the addition of substrates such as leucine, alanine, pyruvate, a leucine-proline mixture, and molecular hydrogen. In the presence of leucine-proline mixture, lindane decomposed in parallel with isovaleric acid formation, and both lindane degradation and isovaleric acid formation were inhibited by monoiodoacetic acid, suggesting a close relation between lindane degradation and the Stickland reaction. Lindane was degraded by cell-free extracts of C. rectum in the presence of dithiothreitol (DTT). Radiogaschromatograms of n-hexane soluble metabolites from [14C] lindane showed the presence of monochlorobenzene and γ-3,4,5,6-tetrachlorocyclohexene, Leucine, NADH, and NADPH were somewhat less active than DTT for lindane degradation in cell-free extracts. Reductive dechlorination seemed the major route of lindane degradation in cell-free extracts as well as in the intact cells of C. rectum.
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00446880
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