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  • Co-transformation  (2)
  • Colletotrichum lindemuthianum  (1)
  • 1
    ISSN: 1432-2242
    Keywords: Transgenic bean ; Phaseolus vulgaris ; Co-transformation ; Biolistic ; Inheritance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Exploiting the biolistic process we have generated stable transgenic bean (Phaseolus vulgaris L.) plants with unlinked and linked foreign genes. Co-transformation was conducted using plasmid constructions containing a fusion of the gus and neo genes, which were co-introduced with the methionine-rich 2S albumin gene isolated from the Brazil nut and the antisense sequence of AC1, AC2, AC3 and BC1 genes from the bean golden mosaic geminivirus. The results revealed a co-transformation frequency ranging from 40% to 50% when using unlinked genes and 100% for linked genes. The introduced foreign genes were inherited in a Mendelian fashion in most of the transgenic bean lines. PCR and Southern blot hybridization confirmed the integration of the foreign genes in the plant genome.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-2242
    Keywords: Key words Transgenic bean ; Phaseolus vulgaris ; Co-transformation ; Biolistic ; Inheritance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Exploiting the biolistic process we have generated stable transgenic bean (Phaseolus vulgaris L.) plants with unlinked and linked foreign genes. Co-transformation was conducted using plasmid constructions containing a fusion of the gus and neo genes, which were co-introduced with the methionine-rich 2S albumin gene isolated from the Brazil nut and the antisense sequence of AC1, AC2, AC3 and BC1 genes from the bean golden mosaic geminivirus. The results revealed a co-transformation frequency ranging from 40% to 50% when using unlinked genes and 100% for linked genes. The introduced foreign genes were inherited in a Mendelian fashion in most of the transgenic bean lines. PCR and Southern blot hybridization confirmed the integration of the foreign genes in the plant genome.
    Type of Medium: Electronic Resource
    Library Location Call Number Volume/Issue/Year Availability
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  • 3
    ISSN: 1573-5060
    Keywords: Phaseolus vulgaris ; dry bean ; Colletotrichum lindemuthianum ; anthracnose ; horizontal resistance ; race-nonspecific resistance ; land race
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Fifty-four land races of dry beans (Phaseolus vulgaris), indigenous to areas of Brazil where anthracnose (caused by Colletotrichum lindemuthianum) is a common problem, were evaluated in field nurseries for partial resistance to race Brazilian 1 (B1) of C. lindemuthianum using symptom severity classes (SSC) from 0 to 6. Plants were selected if symptoms were present and the SSC was less than the 95% confidence interval of the mean SSC of the susceptible cultivar Carioca. S1 progeny from selected plants were evaluated in air-conditioned chambers for partial resistance to races B1, delta, and kappa of C. lindemuthianum. Of 246 S1 families evaluated, 145 families were partially resistant to one or two of the races [symptoms present, but S1 family mean significantly (p〈0.05) less than the mean of Carioca] and susceptible to the third. Six families were partially resistant to all three races. The remaining families were either susceptible or segregated for reaction to race B1. Partial resistance to C. lindemuthianum showed race specificity in the air-conditioned chambers and field nurseries.
    Type of Medium: Electronic Resource
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