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  • Endoderm  (2)
  • Counterflow centrifugation elutriation  (1)
  • 1
    Electronic Resource
    Electronic Resource
    A quantitative method for separation of livingHydra cells (1992)
    Springer
    Development genes and evolution 201 (1992), S. 296-300 
    Details
    ISSN: 1432-041X
    Keywords: Cell separation ; Counterflow centrifugation elutriation ; Cell type specific gene expression ; Hydra
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We describe a rapid method for the isolation of large numbers of livingHydra cells of defined cell type in an isotonic cell medium (Gierer et al. 1972). Intact animals are enzymatically dissociated into a single cell suspension and the various cell types separated in less than one hour by counterflow centrifugation elutriation. Cell loss is minimal. RNA isolated from various fractions can be probed with cell type specific cDNA-clones.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00592110
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
  • 2
    Electronic Resource
    Electronic Resource
    Boundary cells of endodermal origin define the mouth of Hydra vulgaris (Cnidaria) (1995)
    Springer
    Cell & tissue research 280 (1995), S. 235-242 
    Details
    ISSN: 1432-0878
    Keywords: Ectoderm ; Endoderm ; Boundary cells ; Mouth ; Cell adhesion ; Hydra vulgaris (Cnidaria)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract We investigated morphology, dynamics and origin of cells surrounding the mouth of Hydra vulgaris using the monoclonal antibody L96. This antibody recognises a one cell-thick ring of endodermal epithelial cells exactly at the boundary between endoderm (gastrodermis) and ectoderm (epidermis). L96+ cells can stretch considerably without any cell rupture during mouth opening. Thus, our data prove the existence of a distinct cell population defining hydra's mouth. A model for mouth opening is proposed and the significance of L96+ cells for boundary formation between ectoderm and endoderm is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307794
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Boundary cells of endodermal origin define the mouth of Hydra vulgaris (Cnidaria) (1995)
    Springer
    Cell & tissue research 280 (1995), S. 235-242 
    Details
    ISSN: 1432-0878
    Keywords: Key words: Ectoderm ; Endoderm ; Boundary cells ; Mouth ; Cell adhesion ; Hydra vulgaris (Cnidaria)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. We investigated morphology, dynamics and origin of cells surrounding the mouth of Hydra vulgaris using the monoclonal antibody L96. This antibody recognises a one cell-thick ring of endodermal epithelial cells exactly at the boundary between endoderm (gastrodermis) and ectoderm (epidermis). L96+ cells can stretch considerably without any cell rupture during mouth opening. Thus, our data prove the existence of a distinct cell population defining hydra’s mouth. A model for mouth opening is proposed and the significance of L96+ cells for boundary formation between ectoderm and endoderm is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307794
    Library Location Call Number Volume/Issue/Year Availability
    BibTip Others were also interested in ...
    Paper (German National Licenses)
    Fulltext
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