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  • 1
    Electronic Resource
    Electronic Resource
    A quantitative method for separation of livingHydra cells (1992)
    Springer
    Development genes and evolution 201 (1992), S. 296-300 
    Details
    ISSN: 1432-041X
    Keywords: Cell separation ; Counterflow centrifugation elutriation ; Cell type specific gene expression ; Hydra
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We describe a rapid method for the isolation of large numbers of livingHydra cells of defined cell type in an isotonic cell medium (Gierer et al. 1972). Intact animals are enzymatically dissociated into a single cell suspension and the various cell types separated in less than one hour by counterflow centrifugation elutriation. Cell loss is minimal. RNA isolated from various fractions can be probed with cell type specific cDNA-clones.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00592110
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    WNT signalling molecules act in axis formation in the diploblastic metazoan Hydra (2000)
    [s.l.] : Macmillian Magazines Ltd.
    Nature 407 (2000), S. 186-189 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] Members of the Wnt/wingless family of secreted proteins act as short-range inducers and long-range organizers during axis formation, organogenesis and tumorigenesis in many developing tissues. Wnt signalling pathways are conserved in nematodes, insects and vertebrates. Despite its ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/35025063
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  • 3
    Electronic Resource
    Electronic Resource
    Unexpected complexity of the Wnt gene family in a sea anemone (2005)
    [s.l.] : Macmillian Magazines Ltd.
    Nature 433 (2005), S. 156-160 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] The Wnt gene family encodes secreted signalling molecules that control cell fate in animal development and human diseases. Despite its significance, the evolution of this metazoan-specific protein family is unclear. In vertebrates, twelve Wnt subfamilies were defined, of which only six have ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/nature03158
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  • 4
    Electronic Resource
    Electronic Resource
    Identity crisis (1990)
    [s.l.] : Nature Publishing Group
    Nature 346 (1990), S. 21-22 
    Details
    ISSN: 1476-4687
    Source: Nature Archives 1869 - 2009
    Topics: Biology , Chemistry and Pharmacology , Medicine , Natural Sciences in General , Physics
    Notes: [Auszug] SIR-Hydra are used frequently in research and teaching12, but identifying them is extremely difficult because names have been used inconsistently. Recent work has clarified the names and identities of the five species described in Europe3'5. The nematocysts ("stingers") provide the most certain ...
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1038/346021b0
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  • 5
    Electronic Resource
    Electronic Resource
    Boundary cells of endodermal origin define the mouth of Hydra vulgaris (Cnidaria) (1995)
    Springer
    Cell & tissue research 280 (1995), S. 235-242 
    Details
    ISSN: 1432-0878
    Keywords: Key words: Ectoderm ; Endoderm ; Boundary cells ; Mouth ; Cell adhesion ; Hydra vulgaris (Cnidaria)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract. We investigated morphology, dynamics and origin of cells surrounding the mouth of Hydra vulgaris using the monoclonal antibody L96. This antibody recognises a one cell-thick ring of endodermal epithelial cells exactly at the boundary between endoderm (gastrodermis) and ectoderm (epidermis). L96+ cells can stretch considerably without any cell rupture during mouth opening. Thus, our data prove the existence of a distinct cell population defining hydra’s mouth. A model for mouth opening is proposed and the significance of L96+ cells for boundary formation between ectoderm and endoderm is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307794
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  • 6
    Electronic Resource
    Electronic Resource
    Boundary cells of endodermal origin define the mouth of Hydra vulgaris (Cnidaria) (1995)
    Springer
    Cell & tissue research 280 (1995), S. 235-242 
    Details
    ISSN: 1432-0878
    Keywords: Ectoderm ; Endoderm ; Boundary cells ; Mouth ; Cell adhesion ; Hydra vulgaris (Cnidaria)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract We investigated morphology, dynamics and origin of cells surrounding the mouth of Hydra vulgaris using the monoclonal antibody L96. This antibody recognises a one cell-thick ring of endodermal epithelial cells exactly at the boundary between endoderm (gastrodermis) and ectoderm (epidermis). L96+ cells can stretch considerably without any cell rupture during mouth opening. Thus, our data prove the existence of a distinct cell population defining hydra's mouth. A model for mouth opening is proposed and the significance of L96+ cells for boundary formation between ectoderm and endoderm is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00307794
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  • 7
    Electronic Resource
    Electronic Resource
    Determination of nuclear DNA concentration in cells of Myxobolus cerebralis and triactinomyxon spores, the causative agent of whirling disease (1998)
    Springer
    Parasitology research 84 (1998), S. 694-699 
    Details
    ISSN: 1432-1955
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Abstract Myxobolus cerebralis (Myxozoa: Myxosporea) has a complex two-host life cycle, which begins when waterborne triactinomyxon spores released from the infected oligochaete Tubifex tubifex contact a susceptible trout. Upon contact the triactinomyxon spores attach to the fish and release their sporoplasm cells into the epidermis. At approximately 50 days postinfection, sporogenesis begins, resulting in a large number of M. cerebralis spores in the cartilage of infected fish 6 weeks later. The spores of M. cerebralis can be released from infected fish only after the fish die or are eaten by predators. In both cases, spores released into the aquatic environment can be ingested by oligochaete worms of the species T. tubifex and then develop into the actinosporean triactinomyxon stage in the intestine within about 3 months. The triactinomyxon is the only stage infectious for salmonid fish. We determined the DNA concentration in sporoplasm cells, capsulogenic cells, and valvogenic cells of M. cerebralis spore stages from the trout and of triactinomyxon spore stages from T. tubifex. DNA was visualized using the DNA-specific fluorescent stain DAPI. Our results demonstrate that meiosis occurs only once in the developmental cycle of M. cerebralis in contrast to the previously published hypothesis. This takes place within the pansporocyst found in T. tubifex. Thereafter, the sporoplasm cells of the triactinomyxon spores in T. tubifex and M. cerebralis in trout are diploid.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s004360050472
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  • 8
    Electronic Resource
    Electronic Resource
    Nematocyten (1995)
    Weinheim : Wiley-Blackwell
    Biologie in unserer Zeit 25 (1995), S. 161-169 
    Details
    ISSN: 0045-205X
    Keywords: Life and Medical Sciences
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: Die Nematocyten (Nesselzellen) der Cnidaria (Nesseltiere) sind eine der komplexesten und rätselhaftesten aller tierischen Zellen. Ihr charakteristisches Organell, die Nesselkapsel (Nematocyste, Cnide), gehört zu den größten bekannten Sekretionsorganen, und ihre explosive Entladung ist einer der spektakulärsten Prozesse, den man auf Ebene einer Zelle beobachten kann. Tödliche Badeunfälle, die durch Kontakt mit manchen tropischen Würfelquallen hervorgerufen werden, zeigen auch die. Gefährlichkeit des Nesselgiftes. Die Aufklärung von Struktur und Funktion dieser Zellen stellt daher schon lange eine Herausforderung für Biologen und Biophysiker dar. Neue molekular- und zellbiologische Untersuchungen haben uns nun einem Verständnis dieser ungewöhnlichen Zellen näher gebracht.
    Additional Material: 12 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/biuz.19950250314
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