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Digitale Medien

A quantitative method for separation of livingHydra cells (1992)

Greber, Martin J. ; David, Charles N. ; Holstein, Thomas W.

Springer

Development genes and evolution 201 (1992), S. 296-300

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Details

ISSN: 1432-041X

Schlagwort(e): Cell separation ; Counterflow centrifugation elutriation ; Cell type specific gene expression ; Hydra

Quelle: Springer Online Journal Archives 1860-2000

Thema: Biologie

Notizen: Summary We describe a rapid method for the isolation of large numbers of livingHydra cells of defined cell type in an isotonic cell medium (Gierer et al. 1972). Intact animals are enzymatically dissociated into a single cell suspension and the various cell types separated in less than one hour by counterflow centrifugation elutriation. Cell loss is minimal. RNA isolated from various fractions can be probed with cell type specific cDNA-clones.

Materialart: Digitale Medien

URL: http://dx.doi.org/10.1007/BF00592110

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