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  • Cell type specific gene expression  (1)
  • 1
    Electronic Resource
    Electronic Resource
    A quantitative method for separation of livingHydra cells (1992)
    Springer
    Development genes and evolution 201 (1992), S. 296-300 
    Details
    ISSN: 1432-041X
    Keywords: Cell separation ; Counterflow centrifugation elutriation ; Cell type specific gene expression ; Hydra
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary We describe a rapid method for the isolation of large numbers of livingHydra cells of defined cell type in an isotonic cell medium (Gierer et al. 1972). Intact animals are enzymatically dissociated into a single cell suspension and the various cell types separated in less than one hour by counterflow centrifugation elutriation. Cell loss is minimal. RNA isolated from various fractions can be probed with cell type specific cDNA-clones.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00592110
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