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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Langenbeck's archives of surgery 376 (1991), S. 222-227 
    ISSN: 1435-2451
    Keywords: Partial gastrectomy ; B–I partial gastrectomy ; Roux-en-Y partial gastrectomy ; Gastric emptying
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Bei Hausschweinen wurden 2/3-Magenresektionen mit erhaltener sowie ausgeschalteter Duodenalpassage durchgeführt, die Magenentleerung szintigraphisch über 4 h gemessen und einer Vergleichsgruppe nichtresezierter Tiere gegenübergestellt. Für die halbfeste, mit 99mTc markierte Testmahlzeit fanden sich experimentell keine Hinweise für eine verzögerte Magenentleerung nach Ausschaltung der Duodenalpassage durch Roux-Rekonstruktion. B-I- and B-II-Roux-Resektion differierten in der Magenentleerung nicht. Die Längenänderung der bei der Roux-Rekonstruktion verwendeten Jejunumschlinge von 40 auf 20 cm hatte keinen Einfluß auf die Magenentleerung. Die Roux-Rekonstruktion mit trunkulärer Vagotomie führte — bei jedoch großer individueller Streubreite — im Vergleich zur B–I-Resektion zu einer Beschleunigung der Magenentleerung. Die mittlere Restaktivität im Magen betrug nach 240 min für die Kontrollgruppe (n=5) 47,8%, nach B–I-Resektion (n=5) 78,9%, nach Roux-Rekonstruktion mit 40 cm-Schlinge (n=5) 59,0%, nach Roux-Rekonstruktion mit 20 cm-Schlinge (n = 5) 38,1 % and nach Roux-Resektion mit trunkularer Vagotomie (n=4) 20,9%.
    Notes: Summary On domestic pigs 2/3 gastrectomies with retention and elimination of the duodenal passage were carried out. Postprandial gastric emptying was measured scintigraphically for 4 h and compared with a control group (laparotomy only). For the semi-solid, 99mTc-labeled test meal delayed gastric emptying after elimination of the duodenal passage by Roux reconstruction could not be shown. There was no difference in gastric emptying between B-I and Roux-en-Y partial gastrectomy. Also alteration of the length of the jejunum loop from 40 to 20 cm after Roux-en-Y reconstruction had no influence on gastric emptying. Roux reconstruction (40 cm loop) in combination with truncal vagotomy led to a non-uniform gastric emptying, but there was a statistically proven acceleration compared with B-I resection. After 240 min the mean residual intragastric activity of the control group (n=5) was 47.8%, 78.9% after B–I resection (n=5), 59% after Roux reconstruction with 40 cm jejunal loop (n=5), 38.1% after Roux reconstruction with 20 cm jejunal loop (n=5) and 20.9% after Roux-en-Y (40 cm loop) with truncal vagotomy (n = 4).
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1435-2451
    Keywords: Key words Parathyroid tissue ; Cryopreservation ; Xenotransplantation
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Introduction: A modified cryopreservation technique for human parathyroid tissue was compared with the standard method using a programmed freezer. Methods: Total parathyroidectomy was performed in three groups of 6-week-old Rowett nude rats. Group I (control) underwent no transplantation of parathyroid tissue (n=9). After 10 days, the rats of groups II (n=15) and III (n=15) underwent xenotransplantation of 20 mg cryopreserved human parathyroid tissue, which had been stored in liquid nitrogen at –196°C for 1–22 months prior to xenotransplantation. The parathyroid tissue was derived from 15 parathyroidectomized patients with renal hyperparathyroidism. Two tissue samples were obtained from every patient. One sample from every patient was cryopreserved by means of the technique of programmed cryopreservation: programmed freezing of human parathyroid tissue at a controlled rate of –1°C/min to –80°C prior to transfer to liquid nitrogen (group II). The second sample from every patient was cryopreserved by means of a modified cryopreservation technique: immediate placement of human parathyroid tissue in a freezer at –20°C and transfer to liquid nitrogen after 2 h (group III). Calcium and intact parathyroid hormone concentrations in serum were determined over a period of 60 days. Furthermore, individual differences in the calcium concentrations were assessed for each rat on the basis of the calcium levels recorded preoperatively and at day 60. Results: All animals in the control group developed hypocalcemia. Serum calcium concentrations returned to normal levels 60 days after xenotransplantation of parathyroid tissue, which had been cryopreserved using the modified technique (group III) in 12 of 15 rats (80%). At day 60, serum calcium had normalized in 10 of 15 rats (67%) after xenotransplantation of parathyroid tissue cryopreserved using programmed freezing (group II). After modified cryopreservation (group III), the median individual difference in the calcium concentrations was –0.16 mmol/l after programmed freezing (group II). At the end of the study, a median level of human intact parathyroid hormone of 3.5 pg/ml and 2.4 pg/ml was estimated for groups II and III, respectively. There was no statistical significant difference of the individual differences in the calcium concentrations or of the levels of human intact parathyroid hormone between groups II and III. Conclusion: Human parathyroid tissue was successfully xenografted in the present experimental study. The results obtained after cryopreservation using the described modified technique were equivalent to those recorded after controlled freezing in a programmed freezer. The simplified cryopreservation technique therefore appears to be suitable for human parathyroid tissue.
    Type of Medium: Electronic Resource
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