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  • 1
    Electronic Resource
    Electronic Resource
    Maintenance of the induced hypomethylated state of tobacco nuclear repetitive DNA sequences in the course of protoplast and plant regeneration (1994)
    Springer
    Planta 194 (1994), S. 306-310 
    Details
    ISSN: 1432-2048
    Keywords: 5-Azacytidine ; DNA methylation ; DNA (tandem repeats) ; Nicotiana (tissue culture) ; Plant regeneration (tobacco)
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract Previously, we established experimental conditions allowing us to induce hypomethylation of tandem arrays of highly repetitive DNA sequences in the Nicotiana tabacum L. nuclear genome (M. Bezděk et al. 1991, Planta 184, 487–490). In this paper, we demonstrate that loci containing the highly repetitive sequences of the HRS60 family can maintain the induced hypomethylated state through protoplast regeneration, non-differentiated callus growth, and plant regeneration. The hypomethylation, induced with 5-azacytidine and monitored on these sequences, did not substantially alter the capacity of calli to produce plants. It can be concluded that, in contradistinction of multiple copies of transgenes or ectopic genes which are usually recognized as methylation targets, endogenous tandem repeats, such as the HRS60, present at 105 copies in the genome, can escape de-novo methylation.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00197529
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Isolation and characterization of X chromosome-derived DNA sequences from a dioecious plant Melandrium album (1997)
    Springer
    Chromosome research 5 (1997), S. 57-65 
    Details
    ISSN: 1573-6849
    Keywords: DNA methylation ; Melandrium album (syn. Silene latifolia) ; sex chromosomes ; subtelomeric heterochromatin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A number of X chromosome DNA sequences have been isolated from a dioecious plant, Melandrium album (syn. Silene latifolia),using chromosome microdissection followed by degenerate oligonucleotide-primed polymerase chain reaction (DOP–PCR) amplification. Six DNA clones were selected and further characterized by DNA/DNA hybridization techniques to check their copy numbers, sex-specific methylation patterns, species specificity and positions on chromosomes. These clones were moderately to highly repetitive (approximately 103–105 copies per haploid genome) and none of them gave a positive signal on Northern blots. One of the clones yielded a sex-specific methylation pattern: its abundant non-methylated CCGG island was found only in males. All the clones also hybridized to two closely related dioecious Melandrium species (M. rubrum and M. dicline). Nucleotide sequences of two X-derived clones showed a number of internal short direct repeats; one of them strikingly resembled a plant conservative telomere sequence (TTTAGGG).None of the clones hybridized to the X chromosome only, but all were localized at the telomeric heterochromatic regions (DAPI C-bands) of both arms of a vast majority of M. album chromosomes using the fluorescencein situ hybridization (FISH) technique. However, the non-homologousarm of the Y chromosome (contrary to the arm homologous to the X chromosome,possessing the pseudoautosomal region) showed neither a DAPI C-banding-stained heterochromatin nor a FISH signal with any of the DNA probes tested,thus indicating its evolutionary diversification.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1023/A:1011693603279
    Library Location Call Number Volume/Issue/Year Availability
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    Paper (German National Licenses)
    Fulltext
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