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  • 1
    ISSN: 1432-0789
    Keywords: Key words Acetylene inhibition ; Soil core technique ; Denitrification ; Irrigation ; Nitrous oxide entrapment
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract  Two versions of the acetylene inhibition (AI)/soil core method were compared for the measurement of denitrification loss from an irrigated wheat field receiving urea-N at a rate of 100 kg ha–1. With AI/soil core method A, the denitrification rate was measured by analysing the headspace N2O, followed by estimation of N2O dissolved in the solution phase using Bunsen absorption coefficients. With AI/soil core method B, N2O entrapped in the soil was measured in addition to that released from soil cores into the headspace of incubation vessels. In addition, the two methods were also compared for measurement of the soil respiration rate. Of the total N2O produced, 6–77% (average 40%) remained entrapped in the soil, whereas for CO2, the corresponding figures ranged from 12–65% (average 44%). The amount of the entrapped N2O was significantly correlated with the water-filled pore space (WFPS) and with the N2O concentration in the headspace, whereas CO2 entrapment was dependent on the headspace CO2 concentration but not on the WFPS. Due to the entrapment of N2O and CO2 in soil, the denitrification rate on several (18 of the 41) sampling dates, and soil respiration rate on almost all (27 of the 30) sampling dates were significantly higher with method B compared to method A. Averaged across sampling dates, the denitrification rate measured with method B (0.30 kg N ha–1 day–1) was twice the rate measured with method A, whereas the soil respiration rate measured with method B (34.9 kg C ha–1 day–1) was 1.6 times the rate measured with method A. Results of this study suggest that the N2O and CO2 entrapped in soil should also be measured to ensure the recovery of the gaseous products of denitrification by the soil core method.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Biology and fertility of soils 7 (1988), S. 32-38 
    ISSN: 1432-0789
    Keywords: Hydrolysable N ; Mineralizable N ; N2-fixation ; Priming effect ; Plant available N
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Non-symbiotic N2 fixation was studied under laboratory conditions in two soils from Pakistan (Hafizabad silt loam and Khurrarianwala silt loam) and one from Illinois, USA (Drummer silty clay loam) incubated in a 15N-enriched atmosphere. N2 fixation was greatest with the Drummer soil (18–122 μg g−1 soil, depending upon the soil treatment) and lowest with the Khurrarianwala soil (4–81 μg g−1 soil). Fixation was increased by the addition of glucose, a close correlation being observed between the amount of glucose added and the amount of N2 fixed in the three soils (r = 0.96). Efficiency of N2 fixation varied with soil type and treatment and was greatest in the presence of added inorganic P. Application of Mo apparently had a negative effect on the amount and efficiency of N2 fixation in all the soils. The percentage of non-symbiotically fixed 15N in potentially mineralizable form (NH 4 + -N released in soil after a 15-day incubation period under anaerobic conditions) was low (2%–18%, depending upon the soil treatment), although most of the fixed N (up to 90%) was recovered as forms hydrolysable with 6N HCl. Recovery in hydrolysable forms was much greater for the fixed N than for the native soil N, indicating that the former was more available for uptake by plants.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Biology and fertility of soils 31 (2000), S. 270-278 
    ISSN: 1432-0789
    Keywords: Key words Acetylene inhibition ; Denitrification ; Cotton ; Nitrous oxide entrapment ; 15N-balance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Geosciences , Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Abstract  In a 2-year field study, denitrification loss was measured from an irrigated sandy-clay loam under cotton receiving urea-N at 158–173 kg ha–1. An acetylene inhibition-soil core method was employed for the direct measurement of denitrification, considering also the N2O entrapped in the soil. Taking into account the N2O evolved from soil cores and that entrapped in the soil, a total of 65.7 kg N ha–1 and 64.4 kg N ha–1 was lost due to denitrification during the 1995 and 1996 cotton-growing seasons, respectively. Most (〉70%) of the denitrification loss occurred during June–August, a period characterized by high soil temperatures and heavy monsoon rains. On average, 35% of the denitrification-N2O was found entrapped in the soil and the amount of entrapped N2O was significantly correlated with head space N2O concentration and with water-filled pore space. 15N-balance during the 1996 growing season revealed a loss of 71.8 kg N ha–1. It was concluded that a substantial proportion of the fertilizer-N applied to irrigated cotton is lost under the semiarid subtropical climatic conditions prevailing in the Central Punjab region of Pakistan and that denitrification is the major N loss process under irrigated cotton in this region.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1573-5036
    Keywords: C transformation ; Fertilizer N ; Humic components ; Hydrolysable C ; Hydrolysable N ; Immobilization-remineralization of N ; Microbial biomass ; N transformations ; Stabilization of C and N
    Source: Springer Online Journal Archives 1860-2000
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Summary Uniformly14C labelled glucose, cellulose and wheat straw and specifically14C labelled lignin component in corn stalks were aerobically incubated for 12 weeks in a chernozem soil alongwith15N labelled ammonium sulphate. Glucose was most readily decomposed, followed in order by cellulose, wheat straw and corn stalk lignins labelled at methoxyl-, side chain 2-and ring-C. More than 50% of14C applied as glucose, cellulose and wheat straw evolved as CO2 during the first week. Lignin however, decomposed relatively slowly. A higher proportion of14C was transformed into microbial biomass whereas lignins contributed a little to this fraction. After 12 weeks of incubation nearly 60% of the lignin14C was found in humic compounds of which more than 70% was resistant to hydrolysis with 6N HCl. Maximum incorporation of15N in humic compounds was observed in cellulose amended soil. However, in this case more than 80% of the15N was in hydrolysable forms. Immobilization-remineralization of applied15N was most rapid in glucose treated soil and a complete immobilization followed by remineralization was observed after 3 days. The process was much slow in soil treated with cellulose, wheat straw or corn stalks. More than 70% of the newly immobilized N was in hydrolysable forms mainly reepresenting the microbial component. Serial hydrolysis of soil at different incubation intervals showed a greater proportion of 6N HCl hydrolysable14C and15N in fractions representing microbial material.14C from lignin carbons was relatively more uniformly distributed in different fractions as compared to glucose, cellulose and wheat straw where a major portion of14C was in easily hydrolysable fractions.
    Type of Medium: Electronic Resource
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