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  • Ultrastructure  (2)
  • Diagnosis  (1)
  • Galle-Markierung  (1)
  • 1
    ISSN: 1432-0533
    Keywords: Key words GM1-gangliosidosis ; Ultrastructure ; Electrospray ionisation tandem mass spectrometry ; Diagnosis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract The post-mortem diagnosis of lysosomal storage diseases can be confounded by the unavailability of suitable material. Here we report the diagnosis of GM1-gangliosidosis in a cross-bred dog, from which only formalin-fixed brain was available, by a combination of electron microscopy and the detection of elevated levels of GM1-ganglioside within the tissue using the novel technique of electrospray ionisation tandem mass spectrometry. Electron microscopic examination of ultrathin sections of resin-embedded tissue revealed cytoplasmic inclusions (membranous cytoplasmic and zebra bodies) in brain stem and cerebellar neurons that were characteristic of a gangliosidosis. Glycolipids were extracted from the fixed tissue and analysed by tandem mass spectrometry. Two major ions were detected, which corresponded to GM1 (d18:1–C18:0) and GM1 (d20:1–C18:0). Their identity was confirmed by comparison of their fragmentation patterns with those of authentic standards. The concentration of GM1 was approximately sixfold higher on a wet weight basis than in the brain of a normal control dog, confirming the diagnosis of GM1-gangliosidosis.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-1440
    Keywords: duodeno-gastrischer Reflux ; Magensaft ; Galle-Markierung ; Duodenogastric reflux ; Gastric juice ; Bile labelling
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Summary Ten minute samples of gastric juice were collected by naso-gastric aspiration during maximal histamine stimulation in 14 subjects (6 normal, 8 with duodenal ulceration). In eight of these studies, a simultaneous intravenous infusion of Indocyanine green (ICG) was used to label the bile. A single intravenous injection of Boots secretin was given during the maximal plateaux, and further samples collected. The volume, titratable acidity, electrolyte concentrations and ICG concentrations were measured. Changes in volume and in electrolyte concentrations after secretin were consistent with the hypothesis that secretin stimulated duodenogastric reflux, and that duodenogastric reflux was the major determinant of spontaneous variations in electrolyte concentrations in gastric aspirate. Duodenogastric reflux was calculated by a previously described formula based on the sodium content of the aspirate, and this was found to correlate well with the concentration of the ICG label in the aspirate.
    Notes: Zusammenfassung 10 min-Proben von Magensaft wurden mit Hilfe der nasogastrischen Aspiration während maximaler Histaminstimulation bei 14 Personen gesammelt (6 Normalpersonen, 8 Patienten mit Ulcus duodeni). Bei 8 dieser Untersuchungen wurde eine gleichzeitige Infusion mit Indocyanin Grün (ICG) verwendet, um die Galle zu markieren. Eine intravenöse Einzelinjektion von Boots-Sekretin wurde während der maximalen Plateausekretion gegeben und weitere Proben wurden gesammelt. Volumen, titrierbare Azidität, Elektrolyt- und I.C.G.-Konzentrationen wurden gemessen. Veränderungen im Volumen und in den Elektrolytkonzentrationen nach Sekretin stimmten mit der Hypothese überein, daß Sekretin den duodenogastrischen Reflux stimuliert und daß der duodeno-gastrische Reflux die Hauptdeterminante für spontane Variationen in den Elektrolytkonzentrationen im Magenaspirat ist. Der duodeno-gastrische Reflux wurde mit einer früher beschriebenen Formel berechnet, die auf dem Natriumgehalt des Aspirates basiert. Dies erwies sich als gut korreliert mit der Konzentration des ICG im Aspirat.
    Type of Medium: Electronic Resource
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  • 3
    Electronic Resource
    Electronic Resource
    Springer
    Cell & tissue research 128 (1972), S. 303-316 
    ISSN: 1432-0878
    Keywords: Spermatozoon ; Nemertine ; Ultrastructure ; Phylogeny
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Mature spermatozoa of the hoplonemertine, Emplectonema neesii were studied by light and electron microscopy. The spermatozoa are flagellate and motile, each gamete consisting of an elongate anterior head and a posterior flagellar tail. Three regions are identifiable in the head, the acrosome, a nuclear zone and a connecting piece containing two centrioles. The nuclear zone contains glycogen granules as well as an elongate, grooved nucleus and a large mitochondrion whose lobes interdigitate with the nuclear grooves. The flagellum has a typical 9 + 2 flagellar tubule organisation. Nemertine spermatozoan ultrastructure, as exemplified by that of E. neesii, is compared with that of platyhelminth male gametes and the supposed phylogenetic affinity of the two taxeis reexamined in the light of the results of this comparison.
    Type of Medium: Electronic Resource
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