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  • Saccharomyces cerevisiae  (2)
  • Electrophoresis  (1)
  • 1
    Electronic Resource
    Electronic Resource
    The use of electrophoretic karyotypes in the classification of yeasts: Kluyveromyces marxianus and K. lactis (1988)
    Springer
    Current genetics 14 (1988), S. 311-317 
    Details
    ISSN: 1432-0983
    Keywords: Kluyveromyces ; Electrophoresis ; Taxonomy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The relationship between varieties “marxianus” and “lactis” of Kluyveromyces marxianus was investigated. Strains of these varieties readily form hybrids, but their classification in one species has recently been contested. Enzyme patterns and the GC contents of their DNAs differed significantly. Moreover, DNA-DNA reassociation was less than 15%. Since the generally accepted definition of a species is based on gene exchange, we used two approaches for directly detecting genetic recombination in crosses between representatives of both species. First, multiple marked strains were crossed, and the offspring from the resulting hybrids analyzed. Secondly, the fate of individual chromosomes in identical crosses was followed by comparing the karyotypes of the parent strains, the hybrids formed between them, and the descendants of these hybrids. Karyotypes were obtained by orthogonal-field-altemation gel electrophoresis (OFAGE). Gene exchange was not detected with either method. We therefore concluded that the formation of hybrids, even when they produced viable offspring, was not sufficient to include var. “lactis” and var. “marxianus” in one species.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00419987
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Sequence of the open reading frame of the FL01 gene from Saccharomyces cerevisiae (1993)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 9 (1993), S. 423-427 
    Details
    ISSN: 0749-503X
    Keywords: FLO1 ; flocculation ; Saccharomyces cerevisiae ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Notes: The cloned part of the flocculation gene FLO1 of Saccharomyces cerevisiae (Teunissen, A.W.R.H., van den Berg, J.A. and Steensma, H.Y. (1993). Physical localization of the flocculation gene FLO1 on chromosome I of Saccharomyces cerevisiae, Yeast, in press) has been sequenced. The sequence contains a large open reading frame of 2685 bp. The amino acid sequence of the putative protein reveals a serine- and threonine-rich C-terminus (46%), the presence of repeated sequences and a possible secretion signal at the N-terminus. Although the sequence is not complete (we assume the missing fragment consists of repeat units), these data strongly suggest that the protein is located in the cell wall, and thus may be directly involved in the flocculation process.
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320090413
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    Paper (German National Licenses)
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  • 3
    Electronic Resource
    Electronic Resource
    The dominant flocculation genes of Saccharomyces cerevisiae constitute a new subtelomeric gene family (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    Yeast 11 (1995), S. 1001-1013 
    Details
    ISSN: 0749-503X
    Keywords: Flocculation ; Saccharomyces cerevisiae ; FLO1 ; FLO5 ; FLO8 ; FLO9 ; FLO10 ; Life and Medical Sciences ; Genetics
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Biology
    Additional Material: 3 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/yea.320111102
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    Paper (German National Licenses)
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