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  • Engineering  (4)
  • Interphase mapping  (2)
  • Protoplast fusion  (2)
  • 1
    Electronic Resource
    Electronic Resource
    Allotetraploid hybrids between citrus and seven related genera produced by somatic hybridization (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 577-582 
    Details
    ISSN: 1432-2242
    Keywords: Key words  Citrus cultivar improvement ; Germ plasm expansion ; Protoplast fusion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract   We have developed an efficient protoplast-fusion method to produce somatic hybrid allopolyploid plants that combine Citrus with seven related genera, including four that are sexually incompatible. In this paper we report the creation of 18 new allotetraploid hybrids of Citrus, including ten among sexually incompatible related genera, that may have direct cultivar potential as improved citrus rootstocks. All hybrids were confirmed by cytological and RAPD analyses. If fertile, the attributes of these hybrids may be amenable to further genetic manipulation by breeding at the tetraploid level. Wide somatic hybridization of Citrus via protoplast fusion bypasses biological barriers to the natural allopolyploidization of Citrus, and creates new evolutionary opportunities that would be difficult or impossible to achieve by natural or conventional hybridization.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/s001220050166
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    Paper (German National Licenses)
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  • 2
    Electronic Resource
    Electronic Resource
    Allotetraploid hybrids between citrus and seven related genera produced by somatic hybridization (1996)
    Springer
    Theoretical and applied genetics 92 (1996), S. 577-582 
    Details
    ISSN: 1432-2242
    Keywords: Citrus cultivar improvement ; Germ plasm expansion ; Protoplast fusion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract We have developed an efficient protoplast-fusion method to produce somatic hybrid allopolyploid plants that combine Citrus with seven related genera, including four that are sexually incompatible. In this paper we report the creation of 18 new allotetraploid hybrids of Citrus, including ten among sexually incompatible related genera, that may have direct cultivar potential as improved citrus rootstocks. All hybrids were confirmed by cytological and RAPD analyses. If fertile, the attributes of these hybrids may be amenable to further genetic manipulation by breeding at the tetraploid level. Wide somatic hybridization of Citrus via protoplast fusion bypasses biological barriers to the natural allopolyploidization of Citrus, and creates new evolutionary opportunities that would be difficult or impossible to achieve by natural or conventional hybridization.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00224561
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    Paper (German National Licenses)
    Fulltext
  • 3
    Electronic Resource
    Electronic Resource
    Interphase fluorescence in situ hybridization mapping: a physical mapping strategy for plant species with large complex genomes (1996)
    Springer
    Molecular genetics and genomics 252 (1996), S. 497-502 
    Details
    ISSN: 1617-4623
    Keywords: Fluorescence in situ hybridization (FISH) ; Interphase mapping ; Physical mapping ; Maize
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract The chromatin in interphase nuclei is much less condensed than are metaphase chromosomes, making the resolving power of fluorescence in situ hybridization (FISH) two orders of magnitude higher in interphase nuclei than on metaphase chromosomes. In mammalian species it has been demonstrated that within a certain range the interphase distance between two FISH sites can be used to estimate the linear DNA distance between the two probes. The intephase mapping strategy has never been applied in plant species, mainly because of the low sensitivity of the FISH technique on plant chromosomes. Using a CCD (charge-coupled device) camera system, we demonstrate that DNA probes in the 4 to 8 kb range can be detected on both metaphase and interphase chromosomes in maize. DNA probes pA1-Lc and pSh2.5·SstISalI, which contain the maize locia1 andsh2, respectively, and are separated by 140 kb, completely overlapped on metaphase chromosomes. However, when the two probes were mapped in interphase nuclei, the FISH signals were well separated from each other in 86% of the FISH sites analyzed. The average interphase distance between the two probes was 0.50 µm. This result suggests that the resolving power of interphase FISH mapping in plant species can be as little as 100 kb. We also mapped the interphase locations of another pair of probes, ksu3/4 and ksu16, which span theRp1 complex controlling rust resistance of maize. Probes ksu3/4 and ksu16 were mapped genetically approximately 4 cM apart and their FISH signals were also overlapped on metaphase chromosomes. These two probes were separated by an average of 2.32 µm in interphase nuclei. The possibility of estimating the linear DNA distance between ksu3/4 and ksu16 is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02172395
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    Paper (German National Licenses)
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  • 4
    Electronic Resource
    Electronic Resource
    Interphase fluorescence in situ hybridization mapping: a physical mapping strategy for plant species with large complex genomes (1996)
    Springer
    Molecular genetics and genomics 252 (1996), S. 497-502 
    Details
    ISSN: 1617-4623
    Keywords: Key words Fluorescence in situ hybridization (FISH) ; Interphase mapping ; Physical mapping ; Maize
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract  The chromatin in interphase nuclei is much less condensed than are metaphase chromosomes, making the resolving power of fluorescence in situ hybridization (FISH) two orders of magnitude higher in interphase nuclei than on metaphase chromosomes. In mammalian species it has been demonstrated that within a certain range the interphase distance between two FISH sites can be used to estimate the linear DNA distance between the two probes. The interphase mapping strategy has never been applied in plant species, mainly because of the low sensitivity of the FISH technique on plant chromosomes. Using a CCD (charge-coupled device) camera system, we demonstrate that DNA probes in the 4 to 8 kb range can be detected on both metaphase and interphase chromosomes in maize. DNA probes pA1-Lc and pSh2.5⋅SstISalI, which contain the maize loci a1 and sh2, respectively, and are separated by 140 kb, completely overlapped on metaphase chromosomes. However, when the two probes were mapped in interphase nuclei, the FISH signals were well separated from each other in 86% of the FISH sites analyzed. The average interphase distance between the two probes was 0.50 μm. This result suggests that the resolving power of interphase FISH mapping in plant species can be as little as 100 kb. We also mapped the interphase locations of another pair of probes, ksu3/4 and ksu16, which span the Rp1 complex controlling rust resistance of maize. Probes ksu3/4 and ksu16 were mapped genetically approximately 4 cM apart and their FISH signals were also overlapped on metaphase chromosomes. These two probes were separated by an average of 2.32 μm in interphase nuclei. The possibility of estimating the linear DNA distance between ksu3/4 and ksu16 is discussed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF02172395
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    Paper (German National Licenses)
    Fulltext
  • 5
    Electronic Resource
    Electronic Resource
    A super element model for non-linear analysis of stiffened box structures (1993)
    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 36 (1993), S. 2203-2217 
    Details
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: A numerical model for non-linear static and dynamic analysis of stiffened box structures is presented. The model is based on a new super element formulation which provides complete C1 continuity for both plate and beam elements. Geometric and material non-linearities are included and the temporal equations are solved by the implicit Newmark-β method with Newton-Raphson subiteration. The new formulation has been applied to the static, vibration and transient analysis of various structures such as flat plates, folded plates and rectangular boxes. Both isotropic and beam stiffened structures are considered and the results obtained are compared with other available solutions. It is observed that the new super element formulation can provide reasonable solutions to both linear and non-linear problems of stiffened box structures. The mathematical formulation of the model is presented in this paper, while the numerical verifications are given in the companion paper.1
    Additional Material: 5 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/nme.1620361305
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  • 6
    Electronic Resource
    Electronic Resource
    Applications of a super element model for non-linear analysis of stiffened box structures (1993)
    Chichester [u.a.] : Wiley-Blackwell
    International Journal for Numerical Methods in Engineering 36 (1993), S. 2219-2243 
    Details
    ISSN: 0029-5981
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Mathematics , Technology
    Notes: Numerical investigations are carried out to verify the new super element model developed in Reference 1 for non-linear analysis of stiffened box structures. The results presented here are obtained from a variety of examples which are chosen to illustrate the accuracy and convergence of the model in various applications. Good agreement is obtained for most of the problems considered and the present formulation is found to be accurate and economical for preliminary engineering design.
    Additional Material: 28 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/nme.1620361306
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    Paper (German National Licenses)
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  • 7
    Electronic Resource
    Electronic Resource
    Modelling of concrete response to fluctuating load (1989)
    New York, NY [u.a.] : Wiley-Blackwell
    International Journal for Numerical and Analytical Methods in Geomechanics 13 (1989), S. 171-181 
    Details
    ISSN: 0363-9061
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Architecture, Civil Engineering, Surveying , Geosciences
    Notes: An elastoplastic constitutive model for concrete subjected to cyclic loading conditions is presented. The model is an extension of a recently proposed constitutive concept.7 An initial active loading process is described, using the conventional framework of the rate independent theory of plasticity. An appropriate hardening/softening parameter is introduced which allows one to model the sensitivity of material characteristics to varying confining pressure, including a smooth transition from stable to unstable response. For stress histories that include stress reversals, the formulation invokes a set of neutral loading loci defining the conditions of the neutral state.8 The field of hardening moduli is specified through an appropriate interpolation rule which enables one to simulate adequately the effect of cyclic degradation of the material. The performance of the model is verified for some typical cyclic loading histories, and the results are compared with existing experimental data.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/nag.1610130205
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    Paper (German National Licenses)
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  • 8
    Electronic Resource
    Electronic Resource
    Dynamic response of an elastic and viscoelastic full-space to a spherical source (1995)
    New York, NY [u.a.] : Wiley-Blackwell
    International Journal for Numerical and Analytical Methods in Geomechanics 19 (1995), S. 181-193 
    Details
    ISSN: 0363-9061
    Keywords: Engineering ; Engineering General
    Source: Wiley InterScience Backfile Collection 1832-2000
    Topics: Architecture, Civil Engineering, Surveying , Geosciences
    Notes: The dynamic response due to a spherical source of radius a embedded in an elastic and viscoelastic full-space is investigated at a distance R from the source. Previous solutions to the elastic case are extended to incorporate realistic source pressure functions. The elastic solution is then cast in a scale independent form in order to generalize the application. The results show that the near-field of the spherical source may be defined by R/a 〈 5. For this region the particle velocity and displacement decrease as R-2, and the risetime decreases as R-1. However. in the far-field region (R/a 〉 5) the particle velocity and displacement decrease as R-1, and the risetime is independent of R. A non-constant Q model is developed to model viscoelastic attenuation and a complete analytical solution for wave propagation is obtained by cascading the separate mechanisms of geometric attenuation and viscoelastic attenuation. A comparison of our analytical model with the results of dynamic finite element modelling shows excellent agreement. This suggests that the method of cascading the separate transfer functions is a valid approach for wave propagation in viscoelastic media.
    Additional Material: 4 Ill.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1002/nag.1610190303
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