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  • 1
    Electronic Resource
    Electronic Resource
    Palmar fibromatosis (Dupuytren's contracture) (1984)
    Springer
    Virchows Archiv 405 (1984), S. 41-53 
    Details
    ISSN: 1432-2307
    Keywords: Fibromatosis ; Dupuytren's contracture ; Myofibroblast ; Ultrastructure ; Histochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Forty three cases of palmar fibromatosis were studied by light and electron microscopy, enzyme histochemistry, and ultrastructural immunohistochemistry. By electron microscopy most of the cells composing the nodules in both the proliferative and the involutional stages were identical to myofibroblasts. The myofibroblasts in the involutional nodules often possessed microfilament aggregates probably representing contraction of micro(actin)fllaments in the cytoplasm. The proliferative nodules revealed small perivascular haemorrhages and haemosiderin deposits accompanied by accumulation of macrophages and some lymphocytes; these inflammatory cells possibly secrete a certain growth factor inducing proliferation of genetically abnormal fibroblasts and myofibroblasts. Diaminopeptidase IV was detected in myofibroblasts and fibroblasts by enzyme histochemistry and ultrastructural immunohistochemistry; the enzyme may play a role in the metabolism of intercellular substances. Some perivascular mesenchymal cells, interpreted as variants of myofibroblasts, had moderate activity of alkaline phosphatase.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00694924
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  • 2
    Electronic Resource
    Electronic Resource
    Über die Regeneration von Milzautotransplantaten beim Kaninchen. Fermenthistochemische Untersuchungen (1974)
    Springer
    Research in experimental medicine 163 (1974), S. 79-94 
    Details
    ISSN: 1433-8580
    Keywords: Reorganization ; Spleen transplants ; Enzyme histochemistry ; Follicule formation ; Red pulp formation ; Regeneration ; Milztransplantate ; Enzymhistochemie ; Follikelbildung ; Rote Pulpa
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Die Reorganisationsvorgänge in autologen subcutanen Milztransplantaten beim Kaninchen wurden in zeitlich eng gestaffelter Versuchsanordnung untersucht. Zur besseren Strukturanalyse dienten weitgehend spezifische fermentcytochemische Methoden. Die Versuche ergaben, daß innerhalb der ersten 5 Tage eine vollständige Nekrose des Transplantates erfolgte. Gleichzeitig traten, besonders im Transplantatbett, Elemente einer akuten Entzündung mit nachfolgender Granulationsgewebsbildung auf. Am Ende der 2. Woche waren innerhalb der Transplantate erstmals abortive Gitterfasern mit Hilfe der Methenamin-Silber-Reaktion nachweisbar. Zur gleichen Zeit ließen sich neugebildete Sinuswandzellen mit der alpha-Naphthyl-Acetat-Esterase-Reaktion und der Naphthol-AS-Acetat-Esterase-Reaktion selektiv darstellen. Jetzt traten auch erstmals kleine follikuläre Lymphocytenansammlungen auf. Um den 25. Tag erschienen in den zunächst schmalen Pulpasträngen die ersten Makrophagen und Reticulumzellen, die sich vor allem durch ihren hohen Saure Phosphatase-Gehalt auszeichnen. In den darauffolgenden Monaten vollzog sich eine weitere Proliferation dieser Strukturen bis zu einer Wiederherstellung normalen Milzgewebes. Regressive Veränderungen traten nicht auf, auch an 400 Tage alten Transplantaten lagen reguläre morphologische Strukturen vor. Diese Ergebnisse sowie Befunde aus der Literatur lassen am ehesten auf eine Repopulation der zunächst nekrotischen Milztransplantate durch Einwanderung proliferationsfähiger hämatogener Zellen, wahrscheinlich Monocyten, schließen.
    Notes: Summary The process of reorganization in autologous subcutaneous spleen transplants in rabbits was studied at close intervals. Specific enzyme cytochemical methods allowed a more selective structural analysis of the regeneration. The transplants underwent a total necrosis within the first 5 days. The simultaneous appearance of elements of an acute inflammation, especially directly around the transplants, led to the formation of non-specific granulation tissue. At the end of the 2nd week abortive argyrophilic fibers within the transplants were demonstrated with the methenamine-silver-reaction. Newly formed sinus lining cells were revealed at the same time by the alpha-naphthol-acetate-esterase and the naphthol-AS-acetate-esterase reactions. Follicular lymphocyte accumulations could also be detected. On around the 25th day macrophages and reticulum cells appeared in the still narrow pulp cords. These cells were characterized by a high activity for acid phosphatase. In the following months a further proliferation of the splenic structures eventually led to the reformation of normal splenic tissue and structures. Regressive changes did not occur; even after 400 days the transplants showed normal morphological structures. These results as well as those reported in the literature indicate that the repopulation of at first necrotic spleen transplants occurs through the immigration of hematopoetic cells, probably monocytes, which are able to proliferate.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01851447
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  • 3
    Electronic Resource
    Electronic Resource
    Enzymhistochemische, histometrische und ultrastrukturelle Untersuchungen von Milzen bei der Vinylchlorid-Krankheit (1977)
    Springer
    Virchows Archiv 375 (1977), S. 303-317 
    Details
    ISSN: 1432-2307
    Keywords: Vinylchloride-disease ; Splenomegaly ; Electron microscopy ; Enzyme histochemistry ; Morphometrics
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Durch histometrische, enzymhistochemische und elektronenmikroskopische Untersuchungen konnte nachgewiesen werden, da\ die pathologischen MilzverÄnderungen bei der Vinylchlorid-Krankheit nicht durch hepatisch bedingte Pfortaderdruckerhöhung verursacht werden, sondern auf einer Gewebsalteration durch das Vinylchlorid oder dessen Metabolite beruhen. Dabei sind vor allem die faserbildenden Zellen der Milz beteiligt. Faserassoziierte Reticulumzellen in der roten Pulpa und fibroblastische Reticulumzellen in der wei\en Pulpa werden zu übermÄ\iger Bindegewebsneubildung stimuliert. Hierdurch kommt es in der roten Pulpa zur bindegewebigen Verödung der FiltrationsrÄume mit einer Reduzierung der Pulpastrangmakrophagen und in der wei\en Pulpa zu einer Vernarbung der periarteriellen Lymphscheiden. Die Folgen dieser Fibrosierungsprozesse sind charakteristische VerÄnderungen in der quantitativen Zusammensetzung der Gewebskomponenten, die sich deutlich von denjenigen in Milzen bei Lebercirrhose und Milzvenenthrombose unterscheiden.
    Notes: Summary By means of histometric, enzyme histochemical, and electron microscopic investigations it was demonstrated that the pathological changes in the spleen in vinylchloride-disease are primary. Fibroblastic cells are the only specific splenic cells involved. Fibre-associated reticulum cells of the red pulp and fibroblastic reticulum cells in white pulp are stimulated to produce excessive amounts of the extracellular elements of connective tissue, especially collagen fibrils. The newly formed connective tissue causes obliteration of extracellular blood channels in the red pulp and thus a reduction in the number of pulp-cord macrophages, and scarring of the periarterial lymphatic sheaths. The results of this fibrosing process are characteristic quantitative changes in the splenic histologic structures. These changes are different from those structural alterations occuring in spleens following extrasplenic hemodynamic changes, such as thrombosis of the splenic veins or cirrhosis of the liver.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00427061
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  • 4
    Electronic Resource
    Electronic Resource
    Comparative histochemical and electron microscopic studies of the sinus and venous walls of the human spleen with special reference to the sinus-venous connections (1975)
    Springer
    Cell & tissue research 163 (1975), S. 519-533 
    Details
    ISSN: 1432-0878
    Keywords: Human spleen ; Sinus lining cells ; Pulp veins ; Histochemistry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Sinus and venous walls of normal human spleens were studied with enzyme histochemical and electron microscopic methods. Particular attention was paid to the connections between sinuses and veins. Histochemically the sinus lining cells revealed a distinct naphthol-AS-acetate-esterase activity but no reaction for alkaline phosphatase. Venous endothelial cells were positive for the latter but negative for the former enzyme. In the sinusvenous junctional area there were no endothelial cells with reactivity for both enzymes. Electron microscopically both the sinus lining cells and the venous endothelial cells could be clearly characterized and therefore easily distinguished from one another on morphological grounds. There were no clear ultrastructural indications of transitional forms between sinus lining cells and venous endothelial cells in the sinus-venous area. According to these findings, sinus lining cells represent a specialized endothelium, but one with practically no morpholgical similarities to the venous endothelium.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00218497
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  • 5
    Electronic Resource
    Electronic Resource
    Interdigitating cells in the white pulp of the human spleen (1974)
    Springer
    Cell & tissue research 153 (1974), S. 415-417 
    Details
    ISSN: 1432-0878
    Keywords: Spleen (human) ; Periarteriolar lymphocytic sheath ; Reticular cell ; Enzyme histochemistry ; Ultrastructure
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Interdigitating cells are demonstrated as a special type of fixed cell in the periarteriolar lymphocytic sheaths of the human spleen. These cells show typical ultrastructural features as well as a characteristic enzyme histochemical pattern that distinguish them from other reticular cells in the splenic white pulp.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00229168
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  • 6
    Electronic Resource
    Electronic Resource
    The origin of the dendritic reticulum cell (1980)
    Springer
    Cell & tissue research 209 (1980), S. 279-294 
    Details
    ISSN: 1432-0878
    Keywords: Germinal center reaction ; Dendritic reticulum cell ; Rabbit spleen ; Enzyme histochemistry ; Electron microscopy
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary To obtain more information concerning the origin of dendritic reticulum cells, the development of germinal centers in the spleens of rabbits was investigated by conventional light microscopy, enzyme histochemistry, and electron microscopy. Washed sheep erythrocytes were used as antigen. Splenic tissue was examined on the 13th, 18th, 21st, 27th and 48th day after antigen administration. Electron microscopic investigations revealed transitional forms between typical fibroblastic reticulum cells, which formed the framework of the entire splenic white pulp, and typical dendritic reticulum cells. During this transformation, the enzyme histochemical pattern of alkaline phosphatase disappeared and a positive alpha-naphthyl acetate esterase reaction appeared in the transformed cells. On the basis of these findings, it is highly likely that dendritic reticulum cells develop through transformation of fibroblastic reticulum cells during the development of germinal centers in rabbit spleens. The characteristic folding of the surface membrane of dendritic reticulum cells is probably caused by the conspicuous increase in size of the Golgi apparatus, the detachment of vesicles, and the uptake of such vesicles by the cell membrane observed electron microscopically during the cellular transformation. Receptors that are of significance in antigen trapping might reach the cell surface in this manner, i.e., with the Golgi vesicles.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00237632
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