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  • 1
    ISSN: 1432-2242
    Keywords: Triticum aestivum L. ; Doubled-haploids ; Genetics ; Androgenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Plants of three common wheat (Triticum aestivum L. em. Thell) cultivars and one randomly selected doubled-haploid line derived by anther culture from each of the three cultivars were each grown in three environments, a field environment, a greenhouse environment, and a growth chamber environment. Anthers containing largely miduninucleate to late uninucleate microspores were cultured and calli were induced to regenerate plants in order to assess the effects of cultivar, cultivar family (cultivar and corresponding doubled-haploid derivative), anther-donor plant environment, and cultivar X environment interaction on androgenic responses. Large differences in response were observed among cultivars as well as between cultivars and doubled-haploids. Differences between cultivar and doubled-haploid within cultivar family usually resulted from higher frequency of response in the cultivar, contrary to the hypothesis that anther culture per se constitutes a general selective device for superior androgenic responses. Also, in a second experiment, anther callusing frequency was greater in the cultivar ‘Kitt’ than in any of five unique doubled-haploid lines derived from ‘Kitt’. Significant effects were also observed in the first experiment for the interactions of cultivar family X environment as well as doubled-haploid vs. cultivar X environment, although the effect of environment itself was less significant than these interactions.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    Theoretical and applied genetics 62 (1982), S. 155-159 
    ISSN: 1432-2242
    Keywords: Polyhaploids ; Microspore ; Erysiphe graminis tritici ; in vitro androgenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Anthers from three sets of wheat (Triticum aestivum L. em. Thell) F1's and their reciprocal crosses, made between parental lines differing greatly in their ability to produce microspore derived callus, were cultured on the Chinese potato medium so that we could 1) more clearly define the role of nuclear or cytoplasmic factors within T. aestivum in transferring the ability to undergo in vitro androgenesis, and 2) to briefly review the gametic representation and disease screening potential of the resulting polyhaploid wheat plants. The microspore derived calli values from F1's were slightly less than the midparental value. Statistical analysis indicated that the ability of each F1 to produce callus either did not significantly differ from that of the respective parental line having the highest androgenic yield or it exceeded its respective parental line having the lowest yield. No differences were noted between the members of each pair of reciprocal crosses. The results indicate that the transfer of in vitro androgenic ability to F1 hybrids is not dependent upon the maternal cytoplasm source. Polyhaploid plants, carrying the Pm 3 a powdery mildew resistance gene, expressed resistance to culture 4 a of powdery mildew.
    Type of Medium: Electronic Resource
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