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  • 1
    Electronic Resource
    Electronic Resource
    Amplified fragment length polymorphism- and simple sequence repeat-based molecular tagging and mapping of greenbug resistance gene Gb3 in wheat (2002)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 121 (2002), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: The greenbug, Schizaphis graminum (Rondani), is the most economically damaging aphid pest of wheat in the southern Great Plains of the USA. In this study, the single, dominant greenbug resistance gene, Gb3, was molecularly tagged and genetically mapped using amplified fragment length polymorphism (AFLP) and simple sequence repeat(SSR) markers. Three AFLP loci were associated with the Gb3 locus in linkage analysis with 75 F2:3 families from the cross between two near-isogenic lines (NILs) for Gb3,‘TXGBE273’ and ‘TXGBE281′. Two of these loci, XMgcc Pagg and Xmagg Patg cosegregate with Gb3 in the population analysed. Further analysis indicated that XMgcc Pagg and Xmagg Patg are specific for the Gb3 locus in diverse genetic backgrounds. Two SSR markers, Xgwm111 and Xgwm428 previously mapped in wheat chromosome 7D, were shown to be linked with Gb3, 22.5 cM and 33.1 cM from Gb3, respectively, in an F2 population of ‘Largo’בTAM 107’, suggesting that Gb3 is located in the long arm of chromosome 7D. The two AFLP markers cosegregating with Gb3 are valuable tools in developing molecular markers for marker-assisted selection of greenbug resistance in wheat breeding.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1046/j.1439-0523.2002.00693.x
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  • 2
    Electronic Resource
    Electronic Resource
    Multigenic inheritance of biotype-E greenbug resistance in wheat (1995)
    Oxford, UK : Blackwell Publishing Ltd
    Plant breeding 114 (1995), S. 0 
    Details
    ISSN: 1439-0523
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes: Relatively little is known about host-plant insect-resistance genes. Near-isogenic lines (NILs) can be a useful tool in evaluating such genes and their mechanisms of action. Through backcrossing and single-seed selection, NILs have been produced in wheat (Triticum aestivum L.) for resistance/susceptibility to biotype-E greenbug, Schizaphis graminum (Rondani). Crosses among several resistant and susceptible NILs produced segregation patterns in the F2 generation that were inconsistent with simple monogenic inheritance. The simplest genetic hypothesis consistent with the data would involve two independently segregating loci, requiring complementary expression of multiple resistance alleles. F3 segregation data were also consistent with the proposed genetic hypothesis. Multiple alleles are required to account for the observed variation, although the source of the multiple alleles is unknown. Direct comparison of specific resistant/susceptible pairs of NIL's, as well as bulked segregant analysis, should permit elucidation of resistance mechanisms. Based on the segregation data presented, new designations for the resistance loci studied are proposed.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1439-0523.1995.tb00842.x
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  • 3
    Electronic Resource
    Electronic Resource
    The effects of interactions of culture environment with genotype on wheat (Triticum aestivum) anther culture response (1990)
    Springer
    Plant cell reports 8 (1990), S. 525-529 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary The interactions of genotype and several variables related to culture environment, including temperature pretreatment, conditioned medium and agar concentrations were examined in a series of experiments for their effects on percent anthers producing callus and number of embryoids produced per 100 anthers scored. Significant genotypic interaction was observed for both traits with all environmental variables except methods of medium conditioning. Such interactions involve both changes of response magnitude and changes of rank order of genotypes. The highest response frequencies observed were in excess of 30% of anthers callusing. Most lines examined responded relatively well to a culture regime utilizing a 4°C treatment for 7–14 d prior to anther excision, followed by float culture, without transfer and without preconditioning of the culture medium. The results indicate, however, that particular genotypes may have specific requirements with respect to various environmental conditions so that culture conditions may need to be adjusted, especially for the least responsive genotypes.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00820201
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  • 4
    Electronic Resource
    Electronic Resource
    Identification of a wheat (Triticum aestivum) cell line lacking a specific divalent cation requirement (1995)
    Springer
    Plant cell reports 14 (1995), S. 418-422 
    Details
    ISSN: 1432-203X
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Abstract A wheat (Triticum aestivum L.) cell line, derived from anther culture of an F1 hybrid, has exogenous Ca2+, to that of calcium-dependent cells grown on complete medium. The calcium-independent cell line has been grown in the absence of Ca2+ for more than 1.5 years. The cell line grew at a rate similar to that on complete medium for up to 12 weeks, if supplied with any one of the divalent cations, Ca2+, Mg2+, Mn2+, Zn2+, Cu2+ or Co2+, but declined and appeared necrotic when all 6 of these were removed from the medium. The calcium-independence trait, while identified in tissue culture, was also observed in germinated immature embryos of the same hybrid and one of its parental inbred lines.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00234046
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  • 5
    Electronic Resource
    Electronic Resource
    Combining abilities and heritability of callus formation and plantlet regeneration in wheat (Triticum aestivum L.) anther cultures (1984)
    Springer
    Theoretical and applied genetics 68 (1984), S. 131-134 
    Details
    ISSN: 1432-2242
    Keywords: Doubled-haploids ; Diallel ; Tissue culture
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Frequency of callus formation in wheat (Triticum aestivum L. em. Thell) anthers cultured in vitro and the frequency of subsequent plantlet formation from such calli were examined in a diallel population produced from five inbred spring wheat cultivars. Two of the five cultivars were believed to possess relatively high frequencies of response and the other three relatively low response frequencies, based on previous studies. General and specific combining abilities were estimated and found to be highly significant for both traits. Reciprocal effects were also estimated and were highly significant for both traits. Of the 25 entries, the largest mean callus formation frequency was observed on anthers of ‘Kitt’ x ‘Olaf’, while the largest mean plantlet formation frequency was observed using anthers of the cultivar, ‘Fielder’. No significant correlation was observed between the two traits. Heritability estimates in the range of 0.6–0.7 suggested, however, that both traits were highly heritable, so that rapid gain from selection for these traits should be possible. Current limitations due to genetic variation in responses therefore may not constitute a major obstacle to application of in vitro techniques by wheat breeders.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00252328
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  • 6
    Electronic Resource
    Electronic Resource
    Cultivar and cultivar x environment effects on the development of callus and polyhaploid plants from anther cultures of wheat (1984)
    Springer
    Theoretical and applied genetics 67 (1984), S. 273-277 
    Details
    ISSN: 1432-2242
    Keywords: Triticum aestivum L. ; Doubled-haploids ; Genetics ; Androgenesis
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Plants of three common wheat (Triticum aestivum L. em. Thell) cultivars and one randomly selected doubled-haploid line derived by anther culture from each of the three cultivars were each grown in three environments, a field environment, a greenhouse environment, and a growth chamber environment. Anthers containing largely miduninucleate to late uninucleate microspores were cultured and calli were induced to regenerate plants in order to assess the effects of cultivar, cultivar family (cultivar and corresponding doubled-haploid derivative), anther-donor plant environment, and cultivar X environment interaction on androgenic responses. Large differences in response were observed among cultivars as well as between cultivars and doubled-haploids. Differences between cultivar and doubled-haploid within cultivar family usually resulted from higher frequency of response in the cultivar, contrary to the hypothesis that anther culture per se constitutes a general selective device for superior androgenic responses. Also, in a second experiment, anther callusing frequency was greater in the cultivar ‘Kitt’ than in any of five unique doubled-haploid lines derived from ‘Kitt’. Significant effects were also observed in the first experiment for the interactions of cultivar family X environment as well as doubled-haploid vs. cultivar X environment, although the effect of environment itself was less significant than these interactions.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00317054
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  • 7
    Electronic Resource
    Electronic Resource
    Somaclonal variation for freezing tolerance in a population derived from norstar winter wheat (1988)
    Springer
    Theoretical and applied genetics 75 (1988), S. 480-484 
    Details
    ISSN: 1432-2242
    Keywords: Trilicum aestivum ; Winter wheat ; Tissue culture ; Somaclonal variation ; Freezing tolerance
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology
    Notes: Summary Progeny of 66 plants regenerated from callus cultures derived from immature embryos of Norstar winter wheat were evaluated as seedlings for tolerance to controlled freezing. Greater freezing tolerance than the parent cultivar was observed in both R2 and R3 regenerate families. LT50 values (predicted temperatures at which mean survival frequencies are 50%) for four families in the R2 generation and three families in the R3 were significantly lower than that of Norstar. In both R2 and R3 generations, most families did not differ significantly from the cultivar Norstar, by three separate measures of tolerance. Significant variation among families was observed in both R2 and R3 generations for survival, but not for plant height. Variation within family in the R3 generation was also significant, though smaller than that among families. In the R3 generation, eighteen families were significantly less freezing tolerant than Norstar according to LT50, while thirteen were significantly less tolerant according to survival at a minimum temperature of-17 °C.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00276753
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