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  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Journal of molecular medicine 58 (1980), S. 1261-1266 
    ISSN: 1432-1440
    Keywords: Reticulocytes ; Erythrocytes ; Stereometry ; Reflexion contrast ; Reticulocyten ; Erythrocyten ; Stereometrie ; Reflexionskontrast
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Mit dem Reflexionskontrastverfahren, als Zusatzeinrichtung eines Auflichtmikroskops, lassen sich in Giemsa- oder Pappenheim-gefärbten Blutausstrichen Reticulocyten von reifen Erythrocyten unterscheiden. Das Oberflächenrelief der roten Blutzellen ist quantitativ analysierbar und ist bei Schrägbeleuchtung, nach Dezentrierung der Zentralblende, plastisch sichtbar. Reticulocyten sind um etwa 15% größer, zeigen seltener Abweichungen von der Kreisform und eine weniger tiefe zentrale Eindellung als reife Erythrocyten. In supravital mit Brillantkresylblau gefärbten Präparaten sind im Reflexionskontrast um 72% mehr Erythrocyten als Reticulocyten zu diagnostizieren als im Hellfeld.
    Notes: Summary Reticulocytes and erythrocytes are distinguishable in Giemsa-stained blood smears by means of reflexion contrast, an adjunct to a reflected-light microscope. The surface topography of red cells can be quantitatively analyzed; it is directly visible in oblique illumination after decentralization of the central diaphragm. Reticulocytes are about 15% larger and show less deviations from the circular form and a less deep central depression than mature erythrocytes. After supravital staining with brilliant cresyl blue, 72% more red cells can be diagnosed as being reticulocytes in reflexion contrast than in bright field.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1432-0878
    Keywords: Photoperiod ; Pituitary gland, Pars tuberalis ; TSH ; Immunohistochemistry ; Phodopus sungorus
    Source: Springer Online Journal Archives 1860-2000
    Topics: Biology , Medicine
    Notes: Summary Certain secretory cells in the hypophysial pars tuberalis of the Djungarian hamster display marked circannual structural alterations. The present investigation deals with the immunohistochemical properties of this cell group. A distinct TSH-like immunoreactivity was found in secretory cells of this type in the pars tuberalis of animals exposed to long photoperiods, whereas under short photoperiods the TSH-like immunoreactivity was nearly absent. In the pars distalis, the number and distribution of TSH-positive cells did not differ significantly between animals maintained under long and under short photoperiods. LH-and FSH-positive cells could not be detected in the pars tuberalis, but they are clearly present in the pars distalis of both groups of hamsters. Our immunocytochemical results suggest that photoperiodic stimuli influence the secretory activity of TSH-like immunoreactive cells in the pars tuberalis. A connection with the neuroendrocrine-thyroid axis is discussed.
    Type of Medium: Electronic Resource
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