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  • Fibrin adhesive  (1)
  • Gene transfer  (1)
  • Hepatocyte  (1)
  • 1
    Electronic Resource
    Electronic Resource
    Springer
    Transplant international 11 (1998), S. S357 
    ISSN: 1432-2277
    Keywords: Key words MHC ; Hepatocyte ; CTL ; Gene transfer ; Liver
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Previous studies suggest liver transplants can protect other transplanted organs. This effect may be mediated by hepatocytes secreting large amounts of soluble MHC class I antigen. Here our aim was to determine whether immunologic priming by membrane-bound alloantigen could be inhibited by allospecific antigen produced in a secreted form. Cultured Lewis (RT1.Al) hepatocytes were transfected with plasmids encoding either a membrane-bound or secreted form of the alloantigen, RT1.Aa. Cytotoxic T-lymphocyte (CTL) precursor assays were performed on Lewis splenocytes cultured with transfected hepatocytes, or hepatocytes were injected into the portal vein of prospective Lewis recipients of an ACI (RT1.Aa) liver allograft. Results showed CTL priming by membrane-bound RT1.Aa was inhibited in vitro by soluble RT1.Aa. Similarly, acceleration of ACI allograft rejection induced by membrane-bound antigen was abrogated following co-injection of hepatocytes secreting donor alloantigen. In conclusion, production of soluble donor alloantigen by liver transplants may provide protection against the alloimmune response.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1435-2451
    Keywords: Fibrin adhesive ; Temperature ; Enterotomy ; Fibrinkleber ; Temperatur ; Enterotomie
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Zusammenfassung Der Einfluß der Applikationstemperatur des Fibrinklebers (14° C, 37° C) wurde an standardisierten Enterotomien des Rattencolon (n = 18) untersucht und die Klebefestigkeit in vitro bestimmt. Auch bei einer Temperatur von 14° C, bei der Tissucol eben noch anwendbar ist, ergeben sich makroskopisch, mikroskopisch sowie mikroangiographisch keine Hinweise auf eine gestörte Wundheilung. Der subaquale Berstungsdruck als Maβ der Wundfestigkeit war in beiden Gruppen gleich. Für die Reißfestigkeit in vitro haben sich auch keine Unterschiede ergeben. Trotzdem ist auf eine Erwärmung auf Körpertemperatur vor der Applikation stets zu achten.
    Notes: Summary The influence of the application temperature of fibrin adhesives (14°C, 37°C) was studied during standardized enterotomies on the rat colon (n = 18), and the strength of the fibrin clot was evaluated in vitro. There was no macroscopic, microscopic, or microangiographic evidence of a disturbance in wound healing at a temperature of 14°C, at which Tissucol can just barely be applied. Subaqual bursting strength as a parameter for wound healing was the same in both groups, and no differences were found in tensile strength in vitro. Nevertheless, it is advisable always to warm the adhesive before application.
    Type of Medium: Electronic Resource
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