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  • 1
    ISSN: 1432-2277
    Keywords: Key words Kidney transplantation immunology ; Kidney transplantation monitoring ; Flow cytometry crossmatch ; Donor-specific antibodies ; Class I histocompatibility antigens immunology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract This study was designed to investigate the clinical relevance of donor-specific antibodies (DS-Abs) and their influence on graft survival. Among 106 patients who underwent cadaveric kidney donor transplantation and were monitored by flow cytometry crossmatch (FCXM) during the 1st posttransplantation year, 25 (23.6 %) resulted positive for DS-Ab production. During a 2-year follow up only 12 of the 81 FCXM-negative patients (14.8 %) suffered rejection vs 17 of 25 FCXM-positive patients (68 %; P = 0.00 001). Correlating graft loss to DS-Ab production, 9 FCXM-positive patients lost the graft vs only 1 among the FCXM-negative patients. A worse graft function was evidenced in FCXM-positive subjects who had also suffered rejection episodes than in those which had acute rejection but did not produce DS-Abs. A high incidence of HLA-AB mismatches was found in FCXM-positive subjects which produced anti-class I antibodies. FCXM appears useful in estimating posttransplant alloimmune response. Moreover our findings confirm the harmful effects of anti-class I DS-Abs on long-term graft survival.
    Type of Medium: Electronic Resource
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  • 2
    ISSN: 1590-3478
    Keywords: Multiple sclerosis ; T8+cells ; T-cells analysis ; T-cells during clinical phase of MS ; T-lymphocyte subsets ; monoclonal antibodies ; T-cell subsets ; T8+lymphocytes ; blood T-lymphocytes ; lymphocyte subsets
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Sommario Una analisi seriale delle sottopopolazioni T-linfocitarie del sangue periferico è stata eseguita in 280 campioni prelevati in un periodo di 27 mesi da 14 pazienti affetti da Sclerosi Multipla. Una riduzione significativa delle cellule T8+ è stata trovata nel 47,1% (41/87) dei campioni ematici prelevati durante le quattro settimane dopo l'insorgenza della recidiva rispetto al 2,1% (1/47) dei campioni prelevati durante le quattro settimane prima di una recidiva e rispetto al 3,4% (5/146) di quelli prelevati oltre quattro settimane dopo una recidiva. Le variazioni delle sottopopolazioni delle cellule T sembrano correlarsi con l'attività della malattia, ma i valori normali osservati prima delle recidive suggeriscono che la riduzione delle cellule T8+ non può essere usata come un valido predittore delle recidive.
    Notes: Abstract Serial analyses of peripheral blood T-lymphocyte subsets were performed in 280 samples collected over a 27 months period from 14 multiple sclerosis patients. A significant decrease of T8+ cells was found in 47.1% (47/87) of blood samples collected within four weeks of onset of a relapse opposed to 2.1% (1/47) of samples collected during the four weeks before a relapse and to 3.4% (5/146) of those collected over four weeks after a relapse. Changes of T-cells subsets appear to correlate with disease activity, but normal findings observed before relapses suggest that the decrease of T8+ cells cannot be used as valid predictor of relapse.
    Type of Medium: Electronic Resource
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