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  • 1
    ISSN: 1365-3083
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: The role of HLA class I subunits in class Il-restricted immune responses was investigated by means of a panel of monoclonal antibodies (MoAb) recognizing HLA-A,B,C heavy chain and different β2 mitroglobulin (β2m) epitopes. MoAb against either class I subunit strongly inhibited mixed lymphocyte cultures, generation of cytotoxic T lymphocyte cultures, generation of cytotoxic T lymphoytes or natural killer-like activity, and lymphoproliferation in response lo soluble or particulate microbial antigens derived from Candida alhicans. In general. anti-β2m MoAb were more efficient inhibitors than anti-HLA-A, B, C heavy chain MoAb. The inhibitory effects were specific, in that the parental myeloma ascitic fluid or a low-affinity MoAb against β2m, or MoAh directed against non-HLA surface structures did not affect any of the immune responses studied. The MoAb-induced inhibition could not be attributed to nonspecific toxic effects, since PHA-induced blastogenesis and lL-2-dependent proliferation of mixed lymphocyte culture (MLC) blasts were not inhibited. Furthermore, exogenous IL-2 did not reverse the block of MLC and microbial antigen-induced proliferative responses by MoAb. Taken together, these data suggest an involvement ol both subunits of class I antigens in class II-resiticted inmiune reponses.
    Type of Medium: Electronic Resource
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  • 2
    Electronic Resource
    Electronic Resource
    Springer
    International journal of colorectal disease 13 (1998), S. 113-115 
    ISSN: 1432-1262
    Keywords: Key words Pilonidal sinus ; Dufourmentel's technique ; Plastic flaps ; Sacrococcygeal fistula
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Résumé 67 patients porteurs d'un sinus pilonidal chronique ont été traités par excision et lambeau cutané rhomboïde. Une guérison première a été obtenue chez tous les patients à l'exception de deux qui ont développé un sérum et un qui a développé une déhiscence partielle de la plaie en raison d'un hématome ayant nécessité un drainage par la berge du lambeau. La durée moyenne de séjour est de 5.3 jours (1–16). Tous les patients ont repris une activité normale dans les deux semaines qui ont suivi la chirurgie. Aucune récidive tardive n'a été observée au cours d'un follow-up moyen de 74.4 mois (8–137).
    Notes: Abstract Sixty-seven patients with chronic pilonidal sinuses were treated by excision and rhomboid flap transposition (RFT). Primary healing was obtained in all patients except two who developed a seroma and one who had a partial dehiscence of the surgical wound due to a hematoma, which necessitated drainage through the margin of the flap. The average stay was 5.3 days (range 1–16). All patients returned to normal activities within 2 weeks of surgery. No late recurrence occurred after a mean follow-up of 74.4 months (range 8–137).
    Type of Medium: Electronic Resource
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  • 3
    ISSN: 1432-2277
    Keywords: Key words Liver transplantation ; Immunosuppression ; Prednisone
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Prednisone has been commonly considered the mainstay of immunosuppressive therapy after liver transplantation. Recent data suggest that prednisone withdrawal late after transplant reduces complications without affecting graft function. We report here the preliminary results of an open-label, randomized study aimed at investigating whether prednisone therapy can be completely avoided during the first 3 months after transplantation. Twenty-seven consecutive patients were randomized to receive double (group A: cyclosporine and azathioprine) or triple (group B: prednisone, cyclosporine, and azathioprine) immunosuppressive therapy after liver transplantation. Six patients died within the first 3 weeks in each group and were excluded from the calculations. The present results refer to 10 patients in group A and 11 in group B. The actuarial 1-year survival did not differ between the two groups (90.9 % vs 88.8 %). There were no differences with respect to infectious complications or episodes of histological acute graft rejections. Only one severe acute rejection occurred in group A and two in group B. During the first month after transplant, liver and kidney functions tended to be better in the group of patients treated without prednisone, although there were no differences in the mean cyclosporine blood levels. These data, though preliminary, indicate that early immunosuppression without the use of prednisone is safe and tends to be associated with improved liver and renal functions compared to conventional triple therapy.
    Type of Medium: Electronic Resource
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  • 4
    ISSN: 1432-2277
    Keywords: Key words Kidney transplantation immunology ; Kidney transplantation monitoring ; Flow cytometry crossmatch ; Donor-specific antibodies ; Class I histocompatibility antigens immunology
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract This study was designed to investigate the clinical relevance of donor-specific antibodies (DS-Abs) and their influence on graft survival. Among 106 patients who underwent cadaveric kidney donor transplantation and were monitored by flow cytometry crossmatch (FCXM) during the 1st posttransplantation year, 25 (23.6 %) resulted positive for DS-Ab production. During a 2-year follow up only 12 of the 81 FCXM-negative patients (14.8 %) suffered rejection vs 17 of 25 FCXM-positive patients (68 %; P = 0.00 001). Correlating graft loss to DS-Ab production, 9 FCXM-positive patients lost the graft vs only 1 among the FCXM-negative patients. A worse graft function was evidenced in FCXM-positive subjects who had also suffered rejection episodes than in those which had acute rejection but did not produce DS-Abs. A high incidence of HLA-AB mismatches was found in FCXM-positive subjects which produced anti-class I antibodies. FCXM appears useful in estimating posttransplant alloimmune response. Moreover our findings confirm the harmful effects of anti-class I DS-Abs on long-term graft survival.
    Type of Medium: Electronic Resource
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  • 5
    ISSN: 1432-2277
    Keywords: Key words HLA class I matching ; Cadaveric renal transplant ; Crossreactive antigens (CREG) ; Flow cytometric crossmatch (FCXM) ; Anti-HLA alloantibodies
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Donor-recipient HLA matching was retrospectively evaluated in 111 cadaveric renal transplants using Takemoto's ten-residue model in which HLA class I antigens are clustered by crossreactive group (CREGs) on the basis of amino acid sequence homology and the sharing of a particular public epitope. The grade and type of HLA residue mismatching were correlated to posttransplant, class I donor-specific antibody production (monitored by flow cytometry crossmatch), rejection occurrence and clinical outcome during the 1st year posttransplant. In 52 patients with 0 mismatchings (MMs) we observed a low incidence of rejection (11.1 %) and antibody production (11.1 %) for 0 CREG MM grade, while 1 MM was enough to increase immune response against graft (rejection 35 %; antibodies 30 %). Moreover, a significant correlation was observed between Q144, E163, Q62 and L82/R82 epitopes and the incidence of acute rejection and antibody production (“immunogenic” residues) in patients grouped for a single residue mismatch.
    Type of Medium: Electronic Resource
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  • 6
    ISSN: 1590-3478
    Keywords: Multiple sclerosis ; T8+cells ; T-cells analysis ; T-cells during clinical phase of MS ; T-lymphocyte subsets ; monoclonal antibodies ; T-cell subsets ; T8+lymphocytes ; blood T-lymphocytes ; lymphocyte subsets
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Description / Table of Contents: Sommario Una analisi seriale delle sottopopolazioni T-linfocitarie del sangue periferico è stata eseguita in 280 campioni prelevati in un periodo di 27 mesi da 14 pazienti affetti da Sclerosi Multipla. Una riduzione significativa delle cellule T8+ è stata trovata nel 47,1% (41/87) dei campioni ematici prelevati durante le quattro settimane dopo l'insorgenza della recidiva rispetto al 2,1% (1/47) dei campioni prelevati durante le quattro settimane prima di una recidiva e rispetto al 3,4% (5/146) di quelli prelevati oltre quattro settimane dopo una recidiva. Le variazioni delle sottopopolazioni delle cellule T sembrano correlarsi con l'attività della malattia, ma i valori normali osservati prima delle recidive suggeriscono che la riduzione delle cellule T8+ non può essere usata come un valido predittore delle recidive.
    Notes: Abstract Serial analyses of peripheral blood T-lymphocyte subsets were performed in 280 samples collected over a 27 months period from 14 multiple sclerosis patients. A significant decrease of T8+ cells was found in 47.1% (47/87) of blood samples collected within four weeks of onset of a relapse opposed to 2.1% (1/47) of samples collected during the four weeks before a relapse and to 3.4% (5/146) of those collected over four weeks after a relapse. Changes of T-cells subsets appear to correlate with disease activity, but normal findings observed before relapses suggest that the decrease of T8+ cells cannot be used as valid predictor of relapse.
    Type of Medium: Electronic Resource
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