ISSN:
1432-0428
Keywords:
Key words Islet amyloid polypeptide
;
human pancreatic islets
;
islet transplantation
;
immune histochemistry
;
hyperglycaemia.
Source:
Springer Online Journal Archives 1860-2000
Topics:
Medicine
Notes:
Summary Human islets of Langerhans were transplanted to the subcapsular space of the kidneys of nude mice which were either normoglycaemic or made diabetic with alloxan. After 2 weeks, the transplants were processed for light and electron microscopical analyses. In all transplants, islet amyloid polypeptide (IAPP)-positive cells were found with highest frequency in normoglycaemic animals. IAPP-positive amyloid was seen in 16 out of 22 transplants (73 %), either by polarisation microscopy after Congo red staining or by immune electron microscopy. At variance with previous findings of amyloid deposits exclusively in the extracellular space of islets of non-insulin-dependent diabetic patients, the grafted islets contained intracellular amyloid deposits as well. There was no clear difference in occurrence of amyloid between diabetic and non-diabetic animals. The present study indicates that human islets transplanted into nude mice very soon present IAPP-positive amyloid deposits. This technique may provide a valuable model for studies of the pathogenesis of islet amyloid and its impact on islet cell function. [Diabetologia (1995) 38: 543–549]
Type of Medium:
Electronic Resource
URL:
http://dx.doi.org/10.1007/BF00400722
Permalink
