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  • 1
    Electronic Resource
    Electronic Resource
    The influence of amyloid deposits on the islet volume in maturity onset diabetes mellitus (1978)
    Springer
    Diabetologia 15 (1978), S. 417-421 
    Details
    ISSN: 1432-0428
    Keywords: Hyalinosis ; insulin secretion
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Pancreatic islet volumes of patients with and without maturity onset diabetes mellitus were estimated. The islet volume of the diabetic patients was 1.01±0.12 cm3 (SEM) and that of the nondiabetic patients 1.60±0.16 cm3 with considerable overlap between the two groups. Islet amyloidosis was found in all the diabetic and in 9 of the 15 nondiabetic patients. When the amyloid deposits were excluded, the islet volume of the diabetic patients was 0.89±0.10 cm3, while that of the non-diabetic patients was unchanged, 1.60±0.16 cm3. There was still some overlapping. Since amyloid deposits seem to destroy the B cell membranes, it was postulated that a comparison of the volumes of islets completely free of amyloid might give a more true picture of the quantitative islet alterations in maturity onset diabetes. It was found that this islet volume of the diabetics was only 0.41±0.05 cm3 and that of the nondiabetic patients 1.58±0.16 cm3. These values correspond better to the altered insulin secretion in maturity-onset diabetes mellitus.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF01219652
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  • 2
    Electronic Resource
    Electronic Resource
    Islet amyloid in type 2 (non-insulin-dependent) diabetes is related to insulin (1983)
    Springer
    Diabetologia 24 (1983), S. 342-346 
    Details
    ISSN: 1432-0428
    Keywords: Insulin B chain ; immunohistochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Amyloid deposition is the most typical islet alteration in Type 2 (non-insulin-dependent) diabetes. In the present study we show by immunohistochemistry that the amyloid reacts with an antiserum against insulin B chain. Islet amyloid was also purified, dissolved in guanidine-HCl and gel filtered on a Sepharose 6B column. Immunization of a guinea pig with a high molecular weight fraction from this gel filtration resulted in an antiserum with insulin-binding capacity. This binding was partially blocked with pure insulin B chain. The results indicate that islet amyloid contains insulin B chain and that the amyloid is a product of the islet B cells. Thus the study support previous morphological studies.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00251821
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  • 3
    Electronic Resource
    Electronic Resource
    Inhibition of insulin secretion, but normal peripheral insulin sensitivity, in a patient with a malignant endocrine pancreatic tumour producing high amounts of an islet amyloid polypeptide-like molecule (1993)
    Springer
    Diabetologia 36 (1993), S. 843-849 
    Details
    ISSN: 1432-0428
    Keywords: Amylin ; endocrine pancreatic tumour ; glucose tolerance ; insulin resistance ; islet amyloid polypeptide ; pancreatic islets
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Islet amyloid polypeptide or amylin is a polypeptide secreted mainly from the pancreatic beta cells together with insulin upon stimulation. High levels of islet amyloid polypeptide have also been shown to increase the peripheral insulin resistance and consequently a role for islet amyloid polypeptide in the glucose homeostasis has been suggested. We have studied the glucose homeostasis in a patient with a malignant endocrine pancreatic tumour producing large amounts of an islet amyloid polypeptide-like molecule (about 400 times the upper reference level for islet amyloid polypeptide). This patient developed insulin-requiring diabetes mellitus shortly after the tumour diagnosis. Both intravenous and oral glucose tolerance tests revealed inhibited early responses in insulin and C-peptide release, but the insulin and C-peptide response to glucagon stimulation was less affected. Aneuglycaemic insulin clamp showed normal insulin-mediated glucose disposal. In vitro experiments, where isolated rat pancreatic islets were cultured with serum from the patient, showed a moderately decreased islet glucose oxidation rate and glucose-stimulated insulin release compared to islets cultured with serum from healthy subjects. However, culture of rat islets with normal human serum supplemented with synthetic rat islet amyloid polypeptide did not affect the glucose-stimulated insulin release. In conclusion, the observed effects show that the diabetic state in this patient was associated with an impaired glucose-stimulated insulin release but not with an increased peripheral insulin resistance. Thus, the results suggest that if islet amyloid polypeptide has diabetogenic effects they are more likely to be exerted at the level of insulin secretion than at the level of peripheral insulin sensitivity.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00400360
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  • 4
    Electronic Resource
    Electronic Resource
    Co-localization of islet amyloid polypeptide and insulin in the B cell secretory granules of the human pancreatic islets (1989)
    Springer
    Diabetologia 32 (1989), S. 240-244 
    Details
    ISSN: 1432-0428
    Keywords: Islet amyloid polypeptide ; Pancreatic islets ; B cells ; Ultrastructure ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary Islet amyloid polypeptide is a novel 37 amino-acid-residues polypeptide which has been isolated from amyloid deposits in an insulinoma, and in human and cat islets of Langerhans. The molecule has 46% homology with the calcitonin gene-related peptide. Light microscopy examination of the pancreas shows that islet amyloid polypeptide immunoreactivity is restricted to the islet B cells. The present study utilized a rabbit antiserum against a synthetic peptide corresponding to positions 20–29 of islet amyloid polypeptide, a sequence without any amino-acid identity with calcitonin gene-related peptide. By applying the immunogold technique at the ultrastructural level, it was shown that both insulin and islet amyloid polypeptide immunoreactivity occurs in the central granular core of the human B cell secretory granules, while the A cells remain unlabelled. The demonstration that islet amyloid polypeptide is a granular protein of the B cells may indicate that it is released together with insulin. Further studies are necessary to evaluate the functional role of islet amyloid polypeptide.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00285291
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  • 5
    Electronic Resource
    Electronic Resource
    S-100 protein in carcinoid tumours of appendix (1985)
    Springer
    Acta neuropathologica 66 (1985), S. 306-310 
    Details
    ISSN: 1432-0533
    Keywords: Appendix ; Carcinoids ; S-100 protein ; Serotonin ; Immunocytochemistry
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Summary A series of 12 carcinoid tumours of the appendix were examined with regard to S-100 protein immunoreactivity. All tumours were both argentaffin and argyrophil, and displayed immunoreactivity after application of a monoclonal antibody against serotonin. The S-100 protein immunoreactivity appeared in 11 of the 12 tumours, preferably in cells presumably of Schwann cell origin with long slender processes localized at the periphery of the carcinoid tumour buds. Immunoreactive cells with cytoplasmic processes were also seen extending between individual tumour cell in the tumour aggregates. In a few tumours S-100 immunoreactivity occurred in the cytoplasm of tumour cells with or without cytoplasmic extensions. The presence of S-100 protein immunoreactive cells, apparently as an integral component, and its shape and distribution indicate that the peripheral nervous system (PNS) is histogenetically involved in the development of carcinoid tumours of the appendix.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00690963
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  • 6
    Electronic Resource
    Electronic Resource
    Development of polyclonal antibodies and evaluation of a sensitive radioimmunoassay for detection and measurement of synaptophysin (1994)
    Springer
    Acta neuropathologica 87 (1994), S. 635-641 
    Details
    ISSN: 1432-0533
    Keywords: KeyWordsAntibodies ; Immunocytochemistry Radioimmunoassay ; Synaptophysin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Polyclonal antibodies directed towards synaptophysin were raised against a synthesised peptide corresponding to amino acids 246 to 260 of the human synaptophysin sequence. The antibodies, when applied for immunocytochemical staining, showed a staining pattern identical to that of the commercially available monoclonal antibody SY-38. A radioimmunoassay for measurements of synaptophysin was developed using these antibodies and the peptide as standard and tracer. The radioimmunoassay was used for optimising the conditions for purification of synaptophysin from rat brain. No synaptophysin was detected in blood plasma in humans, not even during an embolisation treatment of tumour metastases in the liver, which induced tumour cell necrosis, in a patient with carcinoid tumours. By radioimmunoassay, synaptophysin was detected in cell homogenate from the PC-12 (160 ng/mg) and LCC-18 (40 ng/mg) cell lines and in the cell culture media. In the LCC-18 cell line the synaptophysin immunoreactivity was found in the plasma membrane, and the presence of synaptophysin was confirmed both by radioimmunoassay measurements and by the Northern blot technique. These data indicate that measurements of synaptophysin using this radioimmunoassay are reliable and that the assay can serve as a useful tool in further explorations of the biological effects of synaptophysin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00293325
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  • 7
    Electronic Resource
    Electronic Resource
    Development of polyclonal antibodies and evaluation of a sensitive radioimmunoassay for detection and measurement of synaptophysin (1994)
    Springer
    Acta neuropathologica 87 (1994), S. 635-641 
    Details
    ISSN: 1432-0533
    Keywords: Antibodies ; Immunocytochemistry ; Radioimmunoassay ; Synaptophysin
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Polyclonal antibodies directed towards synaptophysin were raised against a synthesised peptide corresponding to amino acids 246 to 260 of the human synaptophysin sequence. The antibodies, when applied for immunocytochemical staining, showed a staining pattern identical to that of the commercially available monoclonal antibody SY-38. A radioimmunoassay for measurements of synaptophysin was developed using these antibodies and the peptide as standard and tracer. The radioimmunoassay was used for optimising the conditions for purification of synaptophysin from rat brain. No synaptophysin was detected in blood plasma in humans, not even during an embolisation treatment of tumour metastases in the liver, which induced tumour cell necrosis, in a patient with carcinoid tumours. By radioimmunoassay, synaptophysin was detected in cell homogenate from the PC-12 (160 ng/mg) and LCC-18 (40 ng/mg) cell lines and in the cell culture media. In the LCC-18 cell line the synaptophysin immunoreactivity was found in the plasma membrane, and the presence of synaptophysin was confirmed both by radioimmunoassay measurements and by the Northern blot technique. These data indicate that measurements of synaptophysin using this radioimmunoassay are reliable and that the assay can serve as a useful tool in further explorations of the biological effects of synaptophysin.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00293325
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    Paper (German National Licenses)
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  • 8
    Electronic Resource
    Electronic Resource
    Therapy evaluation of neuroendocrine liver metastases with contrast-enhanced MRI (1993)
    Springer
    European radiology 3 (1993), S. 19-25 
    Details
    ISSN: 1432-1084
    Keywords: Liver ; MRI ; Neuroendocrine tumours ; Therapy monitoring
    Source: Springer Online Journal Archives 1860-2000
    Topics: Medicine
    Notes: Abstract Seventeen patients with neuroendocrine liver metastases, 14 of whom were treated with interferon, were examined with MRI before and after contrast administration to evaluate whether there were signal characteristics, differences in homogeneity and/or contrast enhancement patterns that indicated response to or failure of treatment. Of the treated patients 6 objectively responded to treatment (OR), 3 had progressive disease (PD) and 5 had stable disease (SD). A significant difference was found between the SD, untreated (UT) and OR groups of patients in terms of T1 (P = 0.01) and contrast enhancement (P = 0.02). The signal intensity ratio (SIR) in T2-weighted images between tumour and liver was significantly different (P = 0.05) between the OR and PD groups. This indicates that MRI may be used in therapy monitoring of patients with neuroendocrine metastases. Neuroendocrine metastases in the OR group had the same T1 and SIR values as those reported for haemangiomas, while patients in the PD, SD and UT groups had SIR values similar to those for colorectal metastases.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1007/BF00173517
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  • 9
    Electronic Resource
    Electronic Resource
    A novel peptide in the calcitonin gene related peptide family as an amyloid fibril protein in the endocrine pancreas
    Amsterdam : Elsevier
    Biochemical and Biophysical Research Communications 140 (1986), S. 827-831 
    Details
    ISSN: 0006-291X
    Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics: Biology , Chemistry and Pharmacology , Physics
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1016/0006-291X(86)90708-4
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  • 10
    Electronic Resource
    Electronic Resource
    Histopathology of gastric carcinoids: a survey of 42 cases (1984)
    Oxford, UK : Blackwell Publishing Ltd
    Histopathology 8 (1984), S. 0 
    Details
    ISSN: 1365-2559
    Source: Blackwell Publishing Journal Backfiles 1879-2005
    Topics: Medicine
    Notes: An unselected series of 42 gastric carcinoids has been reviewed. Clinically the tumours simulated common gastric lesions including ulcer, polyp and carcinoma. No endocrine symptoms were identified. The tumours were most frequent in the body of the stomach and in 25% in that site were multiple. Morphologically most tumours when classified according to Soga (1974) demonstrated a mixed growth pattern. Six tumours displayed an atypical morphology (type D): they were larger and metastasized more frequently than the rest of the tumours. Six tumours contained a few scattered argentaffinic cells but the others were negative indicating negligible serotonin secretion in only a few cases. The Grimelius argyrophilic reaction was positive in most cells in all tested tumours except in three, two of which showed atypical morphology (type D). It is suggested that gastric carcinoids with a type D morphology or a minority cell population of argyrophil cells are dedifferentiated carcinoids which are biologically nearer to gastric carcinomas. The most frequent clinicopathological correlation was achlorhydria linking pernicious anaemia and gastric carcinoids. This indicates pathogenetic similarities between gastric carcinoids and gastric carcinomas.
    Type of Medium: Electronic Resource
    URL: http://dx.doi.org/10.1111/j.1365-2559.1984.tb02335.x
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